An Enhanced Direct Competitive Immunoassay for the Detection of Kanamycin and Tobramycin in Milk Using Multienzyme-Particle Amplification

Jiang, Ling; Wei, Dali; Zeng, Kun; Shao, Jie; Zhu, Fang; Du, Daolin

doi:10.1007/s12161-018-1185-2

Cited by 41 publications

(25 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The OTA‐mAb‐GNPs probes were prepared as previously described 37,38 . A total of 1 mL of GNP solution was adjusted to the optimal pH via the addition of a 0.2 mol L −1 K 2 CO 3 solution.…”

Section: Methodsmentioning

confidence: 99%

Semi‐quantitative and quantitative detection of ochratoxin A in agricultural by‐products using a self‐assembling immunochromatographic strip

et al. 2020

Self Cite

View full text Add to dashboard Cite

BACKGROUND The toxicity and health risks of mycotoxins have encouraged increased awareness and strict monitoring of these contaminants in agricultural by‐products. In this paper, we developed and tested a sensitive, selective, and self‐assembling immunochromatographic (IC) strip for on‐site detection of ochratoxin A (OTA). We were able to demonstrate semi‐quantitative and quantitative detection of OTA in agricultural by‐product samples. RESULTS The optimized IC strip had a limit of detection (LOD) of 0.5 ng mL−1 OTA using the naked eye for semi‐quantitative detection. When a digitized strip reader was used to achieve quantitative results, the LOD for OTA was reduced to 0.1 ng mL−1 with a linear detection range of 0.1–10 ng mL−1. We also evaluated the specificity, stability, accuracy, and precision of the IC strip and demonstrated high performance in all of these areas. We then confirmed the ability of the IC strip to visually detect OTA contamination in authentic agricultural by‐product samples from the markets in China. Furthermore, quantitative detection of OTA using the IC strip showed that the concentration of OTA ranged from 1.7 to 101.3 ng g−1 in the positive agricultural by‐product samples, correlating well with the measurements obtained via liquid chromatography with tandem mass spectrometry. CONCLUSION The results indicated that this proposed IC strip was capable of sensitive, semi‐quantitative, quantitative, and on‐site detection of OTA contamination in agricultural by‐product samples. © 2020 Society of Chemical Industry

show abstract

Section: Methodsmentioning

confidence: 99%

Semi‐quantitative and quantitative detection of ochratoxin A in agricultural by‐products using a self‐assembling immunochromatographic strip

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…The ZEN-McAb-GNP probes were the essential biosensor materials for reflecting the detection results, which were prepared as per the previously reported method [30,31]. Firstly, appropriate dosages of 0.2 mol/L K 2 CO 3 of an aqueous solution were used to adjust the GNPs solution (1 mL) to pH 8.5.…”

Section: Preparation Of the Zen-mcab-gnp Probesmentioning

confidence: 99%

Contamination of Zearalenone from China in 2019 by a Visual and Digitized Immunochromatographic Assay

Xia

Mao

Yang

et al. 2020

Toxins

Self Cite

View full text Add to dashboard Cite

Zearalenone (ZEN) is a prevalent mycotoxin that needs intensive monitoring. A semi-quantitative and quantitative immunochromatographic assay (ICA) was assembled for investigating ZEN contamination in 187 samples of cereal and their products from China in 2019. The semi-quantitative detection model had a limit of detection (LOD) of 0.50 ng/mL with visual judgment and could be completely inhibited within 5 min at 3.0 ng/mL ZEN. The quantitative detection model had a lower LOD of 0.25 ng/mL, and ZEN could be accurately and digitally detected from 0.25–4.0 ng/mL. The ICA method had a high sensitivity, specificity, and accuracy for on-site ZEN detection. For investigation of the authentic samples, the ZEN-positive rate was 62.6%, and the ZEN-positive levels ranged from 2.7 to 867.0 ng/g, with an average ZEN-positive level being 85.0 ng/g. Of the ZEN-positive samples, 6.0% exceeded the values of the limit levels. The ZEN-positive samples were confirmed to be highly correlated using LC-MS/MS (R2 = 0.9794). This study could provide an efficiency and accuracy approach for ZEN in order to achieve visual and digitized on-site investigation. This significant information about the ZEN contamination levels might contribute to monitoring mycotoxin occurrence and for ensuring food safety.

show abstract

“…CL substrate solutions for HRP (luminol-p-iodophenol and H 2 O 2 ) were obtained from Biokits Technologies Inc (China). The monoclonal antibody against STR (mAb-STR 6H3D5) (Wei et al, 2019) and monoclonal antibody against KAN (mAb-KAN 4G1E5) (Jiang et al, 2018) were prepared in our laboratory and purified with Protein A affinity purification column. Round NCMs with a diameter of 13 mm (0.22 lm) were purchased from Sinopharm Chemical Reagent Beijing Co., Ltd. (Beijing, China).…”

Section: Reagents and Materialsmentioning

confidence: 99%

“…The diluted bovine milks were adopted to prepare the KAN/STR standards to evaluate the matrix effect of milk. And then, five samples of commercially available milk from local markets (Zhenjiang, China) were analysed by both CL imaging immunoassay and UHPLC/MS (Jiang et al, 2018).…”

Section: Analysis Of Bovine Milk Samplesmentioning

confidence: 99%

See 1 more Smart Citation

Chemiluminescence imaging immunoassay for multiple aminoglycoside antibiotics in cow milk

Zeng

Zhang

Wei

et al. 2019

Int J of Food Sci Tech

Self Cite

View full text Add to dashboard Cite

A simple and rapid multiplexed direct competitive chemiluminescent (CL) imaging immunoassay had been developed for the simultaneous detection of kanamycin (KAN) and streptomycin (STR), which were widely used against bacterial infections in animals. To achieve the multiplexed detection of the two targets, a microarray format based on nitrocellulose membranes (NCMs) has been designed. Then, the peroxidase activity of the captured HRP-labelled immunocomplex in each channel was measured by an enhanced luminol-based chemiluminescent solution using a cooled low-light CCD with high resolution. For qualitative analysis, the limit of detection (LOD) was 0.5 ng mL À1 for KAN and 3.13 ng mL À1 for STR by the naked eye. Through the CL data collected, KAN and STR could be detected quantitatively at the LOD of 0.03 and 0.33 ng mL À1 , respectively. This method can be utilised as a screening test to evaluate the presence of antibiotics in milk samples.

show abstract

An Enhanced Direct Competitive Immunoassay for the Detection of Kanamycin and Tobramycin in Milk Using Multienzyme-Particle Amplification

Cited by 41 publications

References 33 publications

Semi‐quantitative and quantitative detection of ochratoxin A in agricultural by‐products using a self‐assembling immunochromatographic strip

Semi‐quantitative and quantitative detection of ochratoxin A in agricultural by‐products using a self‐assembling immunochromatographic strip

Contamination of Zearalenone from China in 2019 by a Visual and Digitized Immunochromatographic Assay

Chemiluminescence imaging immunoassay for multiple aminoglycoside antibiotics in cow milk

Contact Info

Product

Resources

About