An enzyme catalysing the hydrolysis of phosphoric acid diesters in rat liver

In vitro metabolism of desmethyl tetrachlorvinphos by soluble fraction (105 000g) from chicken liver homogenates

“…This step may be identified as an enzyme-catayzed hydrolysis to yield II. Supporting evidence is found in the work of Donninger et al (1971) who have previously observed a similar enzyme system in rat and pig liver which catalyzed the hydrolysis of I to II. Incubation of I with the enzyme preparation produced benzene-soluble metabolites II, III, IV, and V. Metabolites II and III were not detected at 75 min.…”

“…The degradation proceeded in the order dog > rabbit > mouse > rat. Donninger et al (1971) had also reported that mammalian (rat and pig) liver contained an enzyme which catalyzed the hydrolysis of desmethyl tetrachlorvinphos to 2,4,5-trichlorophenacyl chloride.…”

Fate of tetrachlorvinphos and its isomer in soluble fraction (105000g) from goose and turkey liver homogenates

“…The insecticide has been shown to be dealkylated by the soluble fraction from mouse, rat, rabbit, pig (Hutson et al, 1972) and chicken, cow, sheep and pig liver preparation Foster, 1977, 1979) in the presence of glutathione (GSH). It was also observed (Donninger et al, 1971;Akhtar, 1978;Foster, 1977, 1979) that the polar metabolite (desmethyl tetrachlorvinphos) was further metabolized by other enzyme systems to yield additional metabolites. The present report, part of a continuing study on the metabolism of tetrachlorvinphos, details the data on the fate of the insecticide and its isomer in the soluble fraction of liver homogenates from goose and turkey.…”