An enzyme‐containing ink for screen‐printed glucose sensors

Abstract

A glucose sensor employing ferrocene-bound glucose oxidase was fabricated by the screen printing technique. In order to obtain high GOD activity, glucose oxidase (GOD) was modified with ferrocenecarboxylic acid (fc) as an electron mediator. Simultaneous spectroscopic and electrochemical analyses showed that ferrocene-bound GOD (fc-GOD) displayed an increase in catalytic current. The ferrocene acts as an electron transfer relay between the active center of the enzyme and the gold electrode. The screen printing process was used to print successfully the unique fc-GOD containing ink onto the gold electrode patterned on the glass plate. The fc-GOD containing ink was specially developed to be suitable for the printing apparatus. The printed glucose sensor was shown to offer a stable calibration profile and stable electrochemical properties.

Construction of a glucose sensor based on a screen-printed electrode and a novel mediator pyocyanin from Pseudomonas aeruginosa

“…The important advantages of the method were its precision, speed, and cheapness. This technique has been used to construct disposable enzyme electrodes for determination of glucose (Matthews et al, 1987;Higgins, 1990;Newman and Turner, 1992;Nagata et al, 1995;Rohm et al, 1995;Wang et al, 1996), and glutathione (Wring et al, 1991. Generally, the reaction of electron transfer between enzyme molecules and electrode materials is indirectly carried out through another redox compounds such as phenazine methosulfate (PMS), potassium ferricyanide, K 4 [Fe(CN) 6 ], quinines, and Os-complexes because the rapid reaction between the both is very difficult.…”

Recycling Systems Based on Screen-Printed Electrodes

“…This technique is not only suited for the fabrication of base electrodes, but also of whole sensor chips including the biocomponent [20,21]. The fabrication method is cheap and rather simple leading to robust thick film sensors.…”

Enzyme‐Based Bioinorganic Materials

“….) [185,186] were also introduced into the sol-gel matrices or adsorbed to improve the electron transfer from the biomolecules to the conductive support [187,188]. For instance, glucose oxidase has been trapped in organically modified sol-gel chitosan composite with adsorbed ferrocene to construct a low-cost biosensor exhibiting high sensitivity and good stability [189].…”