An enzyme-encapsulated microreactor for efficient theanine synthesis

Abstract

A flow-type microreactor containing glutaminase-mesoporous silica composites with 10.6 nm pore diameter (TMPS10.6) was developed for the continuous synthesis of theanine, a unique amino acid. High enzymatic activity was exhibited by the local control of the reaction temperature.

Advanced characterization of immobilized enzymes as heterogeneous biocatalysts

“…Soaking the support with liquid of comparable refractive index is a possible solution to enable direct measurements also in such cases [12,62,63]. Evidence from CLSM studies on the interaction of proteins with chromatographic supports can be translated almost directly to enzyme immobilization [50,62,[71][72][73][74][75][76][77][78][79][80][81][82][83]. Relationships between the dynamics of protein adsorption and the external and internal geometrical features of the support were established [84][85][86].…”

Dehydration of Lactic Acid: The State of The Art

“…These variations in composition have a very strong impact on the acid and basic properties of solids. In the case of HAP-Ds, calcium deficiency causes a drop in basicity [145] and an increase of acidity [146]. In the case of HAP-Es, an increase of basicity [147] and a drop in acidity [144] could be generally observed.…”

“…All these variations mainly concern site density, but a similar variations in site strength to a lesser extent can be also observed. Matsuura et al have studied the impact of the variation of the Ca/P ratio associated with the partial substitution of calcium by sodium [146,148], as well as the effect of the substitution of calcium by Pb or Sr and P by V [150] for HAPs prepared by hydrothermal synthesis. The authors obtains excellent yields (in the order of 70 %) of acrylic acid with the catalyst Ca-Na-HAP(1.55) (Ca/P = 1.55; (Ca+Na)/ P = 1.65), with a remarkable stability over time (Fig.…”

A Falling‐Film Microreactor for Enzymatic Oxidation of Glucose

“…Some recent articles, such as that of Matsuura et al, have reported enzyme-encapsulated microreactors for efficient synthesis. [21] Zhang et al used an open tubular microreactor with an enzyme-functionalized microfluidic channel for the amperometric detection of glucose. [22] The linear range for the detecMany oxidation processes require the presence of molecular oxygen in the reaction media.…”

“…Hence, microchannels were packed with encapsulated enzymes and called "packed-bed micro-bioreactors." [21,23,24] In this paper, we demonstrate that a continuously operated microreactor system can be used for oxidative enzyme catalysis even without immobilization. For the appropriate test reaction, the biocatalyst should have high activity and a moderate price.…”