An enzyme-free electrochemical biosensor based on NiCoP@PtCu nanozyme and multi-MNAzyme junctions for ultrasensitive Uracil-DNA glycosylase detection

Tian, Guozhen; Li, Wenting; Liu, Biao; Xiao, Meifang; Xia, Qianfeng

doi:10.1016/j.snb.2022.133224

Sensors and Actuators B: Chemical

2023

DOI: 10.1016/j.snb.2022.133224

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An enzyme-free electrochemical biosensor based on NiCoP@PtCu nanozyme and multi-MNAzyme junctions for ultrasensitive Uracil-DNA glycosylase detection

Guozhen Tian

Wenting Li

Biao Liu³

et al.

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Cited by 8 publications

References 34 publications

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Toehold region triggered CRISPR/Cas12a trans‐cleavage for detection of uracil‐DNA glycosylase activity

Cui,

Guo,

Chen

2024

Biotechnology Journal

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DNA glycosylases are a group of enzymes that play a crucial role in the DNA repair process by recognizing and removing damaged or incorrect bases from DNA molecules, which maintains the integrity of the genetic information. The abnormal expression of uracil‐DNA glycosylase (UDG), one of significant DNA glycosylases in the base‐excision repair pathway, is linked to numerous diseases. Here, we proposed a simple UDG activity detection method based on toehold region triggered CRISPR/Cas12a trans‐cleavage. The toehold region on hairpin DNA probe (HP) produced by UDG could induce the trans‐cleavage of ssDNA with fluorophore and quencher, generating an obvious fluorescence signal. This protospacer adjacent motif (PAM)‐free approach achieves remarkable sensitivity and specificity in detecting UDG, with a detection limit as low as 0.000368 U mL−1. Moreover, this method is able to screen inhibitors and measure UDG in complex biological samples. These advantages render it highly promising for applications in clinical diagnosis and drug discovery.

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Toehold region triggered CRISPR/Cas12a trans‐cleavage for detection of uracil‐DNA glycosylase activity

Cui,

Guo,

Chen

2024

Biotechnology Journal

View full text Add to dashboard Cite

show abstract

Advances in enzyme-free nucleic acid amplification-based fluorescent biosensors for real-time imaging of DNA repair enzymes in living cells

Zhang,

Su,

Qiu

et al. 2023

Coordination Chemistry Reviews

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A simple enzyme-free co-system based on Mg²⁺-dependent deoxyribonuclease fluorescent aptasensor for AFB1 sensitive detection

Lu,

Dong,

Han

et al. 2024

Food Bioscience

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An enzyme-free electrochemical biosensor based on NiCoP@PtCu nanozyme and multi-MNAzyme junctions for ultrasensitive Uracil-DNA glycosylase detection

Cited by 8 publications

References 34 publications

Toehold region triggered CRISPR/Cas12a trans‐cleavage for detection of uracil‐DNA glycosylase activity

Toehold region triggered CRISPR/Cas12a trans‐cleavage for detection of uracil‐DNA glycosylase activity

Advances in enzyme-free nucleic acid amplification-based fluorescent biosensors for real-time imaging of DNA repair enzymes in living cells

A simple enzyme-free co-system based on Mg²⁺-dependent deoxyribonuclease fluorescent aptasensor for AFB1 sensitive detection

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