An enzyme immobilization platform for biosensor designs of direct electrochemistry using flower-like ZnO crystals and nano-sized gold particles

Zhang, Yuwei; Zhang, Yun; Wang, Hua; Yan, Bani; Shen, Guo‐Li; Yu, Ru‐Qin

doi:10.1016/j.jelechem.2008.12.010

Cited by 63 publications

(26 citation statements)

References 37 publications

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“…The peak at 531 cm -1 , 571 cm -1 (ZnO 300°C) and 579 cm -1 (ZnO 500°C) can be assigned to A 1(LO) and E 1(LO) vibration mode. The sample absorption maybe the reason for the decrease of the Raman peaks [4, 46,47]. The broadened weak peak at 579 cm -1 (ZnO 500°C) appeared in the spectra is usually related with the defects of the O-vacancy and Zn interstitial [4,11].…”

Section: Raman Spectroscopymentioning

confidence: 99%

The Study of Structural, Physical and Electrochemical Activity of Zno Nanoparticles Synthesized by Green Natural Extracts of Sageretia Thea

Mayedwa¹,

Khalil²,

Mongwaketsi³

et al. 2017

Nano Res Appl

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In this contribution an exceptionally simple, cost effective and reliable method for biosynthesis of ZnO nanoparticles through using Sageretia thea natural extracts as an effective chelating agent. The morphological analysis shows ZnO has nanoscale particles size 28.09 nm ± 5 nm, crystalline and possess distinct nanostructure hexagonal wurtzite structure. Effects of heat on ZnO synthesised at room temperature caused bonds with in the molecule to be broken exhibited continuous weight loss with 3 quasi sharp changes and 3 endothermic peaks occurring at 79°C, 228°C and 300°C. A proposed mechanism of reaction for Zn(NO 3 ) 2 6H 2 O precursor with bioactive compounds in Sageretia thea extract such as phenolic acid, flavonoids, natural sterolin and vitamin based compounds to form ZnO nanoparticles. Electrochemically ZnO nanoparticles are electroactive by exhibiting charge transfer resistance (R ct ) 82006 Ω and bare GCE 3.7707 × 10 5 Ω. Showed good catalytic and conductivity which can be applied for pseudo capacitors.

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Section: Raman Spectroscopymentioning

confidence: 99%

The Study of Structural, Physical and Electrochemical Activity of Zno Nanoparticles Synthesized by Green Natural Extracts of Sageretia Thea

Mayedwa¹,

Khalil²,

Mongwaketsi³

et al. 2017

Nano Res Appl

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“…Moreover, the gold nanosphere-mesoporous silica composite represented excellent catalytic behavior to the oxidation of glucose, faster response time, lower detection limit, higher sensitivity and wider linear range with respect to solely use gold nanosphere. Zhang et al reported that flower-like ZnO crystal was synthesized to be employed for anchoring hoseradish peroxidase (HRP)-labeled gold nanospheres (15). The introduction of flower-like ZnO crystals into chitosan matrix to be casted on the electrode could offer greatly increased loading surface area available for binding gold nanosphere and HRP in a larger scale.…”

Section: Gold Nanoparticlesmentioning

confidence: 99%

“…Inorganic nanomaterials also possess some outstanding properties of http://bmbreports.org large surface-to-volume ratio and high electrical conductivity that provides electron communication of electrical signals (15,69,70). Thus recent years witness the vigorous applications of inorganic nanomaterials in the development of biosensors (71).…”

Section: Biosensorsmentioning

confidence: 99%

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Inorganic nanomaterial-based biocatalysts

Lee

Lee²,

Chang³

et al. 2011

BMB Reports

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Over the years, nanostructures have been developed to enable to support enzyme usability to obtain highly selective and efficient biocatalysts for catalyzing processes under various conditions. This review summarizes recent developments in the nanostructures for enzyme supporters, typically those formed with various inorganic materials. To improve enzyme attachment, the surface of nanomaterials is properly modified to express specific functional groups. Various materials and nanostructures can be applied to improve both enzyme activity and stability. The merits of the incorporation of enzymes in inorganic nanomaterials and unprecedented opportunities for enhanced enzyme properties are discussed.

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“…The threefold sensitivity enhancement (compared with the biosensor not containing ZnO) was attributed to the ZnO film facilitating the formation and distribution of the Ag NPs. HRP was effectively immobilized, based on the studies of the flower-like ZnO-Au NP-Nafion composite [93,94]. The biosensor demonstrated direct electrochemistry with enhanced electrocatalytic activity towards the reduction of H 2 O 2 .…”

Section: Detection Of Other Analytesmentioning

confidence: 99%

ZnO nanostructures in enzyme biosensors

et al. 2015

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Biosensing has developed tremendously since it was demonstrated by Leland C. Clark Jr. in 1962. ZnO nanomaterials are attractive candidates for fabricating biosensors, because of their diverse range of nanostructures, high electron mobility, chemical stability, electrochemical activity, high isoelectric points which promote enzyme adsorption, biocompatibility, and piezoelectric properties. This review covers ZnO nanostructures applied in enzyme biosensors, in the light of electrochemical transduction and field effect transduction. Different assembly processes and immobilization methods have been used to load enzymes into various ZnO nanostructures, providing enzymes with favorable micro-environments and enhancing their sensing performance. We briefly describe recent trends in ZnO syntheses, and the analytical performance of the fabricated biosensors, summarize the advantages of using ZnO nanostructures in biosensors, and conclude with future challenges and prospects.

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An enzyme immobilization platform for biosensor designs of direct electrochemistry using flower-like ZnO crystals and nano-sized gold particles

Cited by 63 publications

References 37 publications

The Study of Structural, Physical and Electrochemical Activity of Zno Nanoparticles Synthesized by Green Natural Extracts of Sageretia Thea

The Study of Structural, Physical and Electrochemical Activity of Zno Nanoparticles Synthesized by Green Natural Extracts of Sageretia Thea

Inorganic nanomaterial-based biocatalysts

ZnO nanostructures in enzyme biosensors

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