An Enzyme-Linked Immunosorbent Assay for the Detection of Specific IgG Antibody toMycoplasma Gallisepticumin Sera and Tracheobronchial Washes

Abstract

A sensitive indirect ELISA is reported for the detection and quantitation of specific IgG to Mycoplasma gallisepticum (MG) in sera and tracheobronchial washes (TBW) of MG-infected chickens. The sensitivity of the assay was ensured by the use of mouse monoclonal antibody to chicken IgG bound to a prospective anti-MG containing sample that was complexed with MG antigen immobilized on a solid phase. The level of specific IgG antibody in a test sample was detected by using peroxidase-conjugated goat anti-mouse IgG. Serum samples with various levels of anti-MG IgG activity were used to construct a standard curve response at a single working dilution by using Logit-Log curve fitting. The assay was simple, reliable, and specific and was used to monitor the appearance of specific anti-MG IgG in chicken sera and TBW at various intervals after the onset of mycoplasma-induced respiratory disease. The IgG response reached a plateau at 2 and 4 weeks postinfection in TBW and sera, respectively; then the response waned but still was detectable at a significant level for up to 25 weeks postinfection.

Seroprevalence of mycoplasmosis in broiler, layer, and native chickens in Giza, Egypt

“…As a more precise measure, ELISA may differentiate between false and true positive reactions. ELISA was identified as an easy, effective, and specific assay in related studies, and it was used to track the appearance of specific anti- Mg IgG in chicken sera at different intervals after the onset of mycoplasma-induced respiratory disease [ 18 ]. Antibodies to MS were also detected using ELISA in the egg yolks of commercial layers as proof of MS infection.…”