An enzyme-linked immunosorbent assay for the detection of antibody to avian reovirus by using protein σ B as the coating antigen

Shien, Jui Huang; Yin, Hao; Lee, L.H

doi:10.1053/rvsc.2000.0414

Cited by 25 publications

(6 citation statements)

References 23 publications

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“…It is also an interesting approach in the case of non-cultivable or difficult cultivable viruses as shown in this study. Following the serological diagnosis of viruses of the family reoviridae belonging to genera Coltivirus (Mohd Jaafar et al, 2003), Orbivirus (Idrissi Bourgine et al, 1999;Nagesha et al, 1996;Yamakawa and Furuuchi, 2001), Orthoreovirus (Liu et al, 2002;Shien et al, 2000), Rotavirus (Castello et al, 2002;Ray et al, 2003;Steele and James, 1999) and Seadornavirus using recombinant proteins, this study shows that a specific recombinant protein of the EYAV can be used as an antigen for detecting specific antibodies to the virus.…”

Section: Discussionmentioning

confidence: 85%

Recombinant VP6-based enzyme-linked immunosorbent assay for detection of immunoglobulin G antibodies to Eyach virus (genus Coltivirus)

Jaafar¹,

Attoui²,

Micco³

et al. 2004

Journal of Clinical Virology

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Section: Discussionmentioning

confidence: 85%

Recombinant VP6-based enzyme-linked immunosorbent assay for detection of immunoglobulin G antibodies to Eyach virus (genus Coltivirus)

Jaafar¹,

Attoui²,

Micco³

et al. 2004

Journal of Clinical Virology

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“…ARV σB, the major outer capsid protein, which interacts with μB to form the outer capsid, appears to be responsible for early virus–host interactions in ARV infection and produces group-specific antibody against ARV [ 4 , 27 ]. Although there are a few reports on the development of ARV vaccines and diagnostic reagents based on σB [ 38 ], there is no report on the properties and activities of this protein or its role in viral pathogenesis. In this study, ARV capsid protein σB was found to associate with Cav-1 in DRMs fractions, suggesting that the ARV σB is probably associated with caveolar lipid rafts at the ARV entry stage.…”

Section: Discussionmentioning

confidence: 99%

Avian Reovirus σB Interacts with Caveolin-1 in Lipid Rafts during Dynamin-Dependent Caveolae-Mediated Endocytosis

Wang

Zhang

Zuo

et al. 2022

Viruses

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Caveolin-1 (Cav-1) is the basic component of caveolae, a specialized form of lipid raft that plays an essential role in endocytic viral entry. However, the evidence of direct involvement of caveolae and Cav-1 in avian reovirus (ARV) entry remains insufficient. In this study, the membrane lipid rafts were isolated as detergent-resistant microdomains (DRMs) by sucrose gradient centrifugation, and the capsid protein σB of ARV was found to associate with Cav-1 in DRMs fractions. Additionally, the interaction between ARV σB protein and Cav-1 was demonstrated by immunofluorescence co-localization and co-immunoprecipitation assays. Furthermore, we found that the internalization of ARV is sensitive to caveolae and dynamin inhibitors, while it is insensitive to clathrin inhibitors. In conclusion, these results indicate that the ARV σB protein interacts with Cav-1 during dynamin-dependent caveolae-mediated endocytosis for the entry of ARV.

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“…A recombinant VP6 rotavirus group C protein-based ELISA has been developed and used in diagnostic assays (8,25). Among the orthoreoviruses, recombinant óB and óC proteins have been used in the diagnosis of avian reoviruses (19,24). Serological tests based on recombinant VP7 and NS3 have also been developed for viruses of the genus Orbivirus (African horse sickness virus, epizootic hemorrhagic disease virus, and Palyam virus) (16,21,27).…”

Section: Discussionmentioning

confidence: 99%

Recombinant VP7-Based Enzyme-Linked Immunosorbent Assay for Detection of Immunoglobulin G Antibodies to Colorado Tick Fever Virus

Jaafar¹,

Attoui²,

Gallian³

et al. 2003

J Clin Microbiol

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VP6, VP7, VP9, VP10, VP11, and VP12 of Colorado tick fever virus (CTF virus), a virus member of the genus Coltivirus, family Reoviridae, were expressed in bacteria with the pGEX-4T-2 vector. A partial sequence of VP7 (designated pVP7) was chosen to elaborate an enzyme-linked immunosorbent assay (ELISA) for detecting anti-CTF virus immunoglobulin G (IgG) antibodies in humans. This was based on two observations: (i) among all expressed proteins, pVP7 showed the highest immunoreactivity to an anti-CTF virus hyperimmune ascitic fluid; (ii) to provide the highest selectivity of antibody detection, the expressed sequence was chosen within a region which is highly divergent (49% amino acid identity) from the homologous sequence of another coltivirus, the Eyach virus. The pVP7 ELISA was evaluated with 368 serum samples from French blood donors and found to provide 98.1% specificity. Assays with the Calisher set of human serum samples, positive for anti-CTF virus antibodies (C. H. Calisher, J. D. Poland, S. B. Calisher, and L. A Warmoth, J. Clin. Microbiol. 22:84-88, 1985), showed that the pVP7 ELISA provided 100% sensitivity for the tested population. After elaboration of recombinant-protein-based ELISAs for diagnosis of infections with members of the viral genera Orbivirus, Orthoreovirus, and Rotavirus, it was shown that a recombinant protein could be used to detect antibodies to the human pathogen Colorado tick fever virus.

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An enzyme-linked immunosorbent assay for the detection of antibody to avian reovirus by using protein σ B as the coating antigen

Cited by 25 publications

References 23 publications

Recombinant VP6-based enzyme-linked immunosorbent assay for detection of immunoglobulin G antibodies to Eyach virus (genus Coltivirus)

Recombinant VP6-based enzyme-linked immunosorbent assay for detection of immunoglobulin G antibodies to Eyach virus (genus Coltivirus)

Avian Reovirus σB Interacts with Caveolin-1 in Lipid Rafts during Dynamin-Dependent Caveolae-Mediated Endocytosis

Recombinant VP7-Based Enzyme-Linked Immunosorbent Assay for Detection of Immunoglobulin G Antibodies to Colorado Tick Fever Virus

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