An enzymic and centrifugal method for estimating high-density lipoprotein cholesterol.

Allen, Janet K.; Hensley, W.J.; Nicholls, Alyssa; Whitfield, John B.

doi:10.1093/clinchem/25.2.325

Cited by 64 publications

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“…HDL fraction was obtained by precipitation with 20% polyethylene glycol (14). Cholesterol and triglyceride concentrations from whole plasma and lipoprotein fractions were assayed enzymatically (Monotest and GPO-PAP, Boehringer Mannheim GmbH, Germany).…”

Section: Methodsmentioning

confidence: 99%

Severe hyperlipoproteinemia in congenital nephrotic syndrome of the Finnish type: effect of dialysis and kidney transplantation

et al. 1993

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Two children with congenital nephrosis of the Finnish type were studied successively at the three stages of the disease: (A) nephrosis, (B) renal insufficiency/peritoneal dialysis and (C) post-transplantation; two additional patients were studied at two stages. Plasma lipoprotein profiles were determined by density gradient ultracentrifugation and lipids by enzymatic methods. Stage A was characterized by hyperchylomicronemia, low high density lipoprotein (HDL) cholesterol and the presence of dense low density lipoprotein (LDL) and HDL particles. Total cholesterol and triglycerides showed great daily variation (5-14 and 5-33 mmol/l, respectively). During stage B, hyperlipidemia weakened. Yet HDL concentration remained low and the concentration of intermediate density lipoproteins (IDL) increased. At stage C, hyperlipidemia had almost subsided, but the presence of IDL persisted. In conclusion, severe hyperlipoproteinemia of congenital nephrosis at the nephrotic stage is attenuated during renal insufficiency and dialysis, and essentially normalizes after kidney transplantation. Yet the presence of IDL implies an increased risk of atherosclerosis.

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Section: Methodsmentioning

confidence: 99%

Severe hyperlipoproteinemia in congenital nephrotic syndrome of the Finnish type: effect of dialysis and kidney transplantation

et al. 1993

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“…Phospholipid levels were determined in terms of choline levels by an enzymatic method (PL B kit, Wako Chemical, Japan) (Takayama et al, 1977). HDL cholesterol was assayed after precipitation with MnC12 (Allen et al, 1979). The plasma glucose levels were measured by the glucose oxidase method (Banauch et al, 1975).…”

Section: Determination Ofplasma Levels Of Glucose and Lip Idsmentioning

confidence: 99%

Lysophosphatidylcholine molecular species in low density lipoprotein and high density lipoprotein in alloxan-induced diabetic rats: effect of probucol

Shi

Yoshinari²,

Iino³

et al. 2009

Exp Clin Endocrinol Diabetes

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To elucidate the contribution of diabetic state toward the accumulation of lysophosphatidylcholine (LPC) in low density lipoprotein (LDL), the LPC molecular species in LDL and high density lipoprotein (HDL) obtained from alloxan-induced diabetic rats were determined by high performance liquid chromatography. The palmitoyl LPC (PLPC) per protein was increased in the LDL of diabetic rats (p < 0.05), whereas the stearoyl LPC (SLPC) decreased in both the LDL and HDL of diabetic rats (p < 0.001) in comparison to nondiabetic rats. After the dietary administration of probucol for 4 weeks, the concentrations of SLPC and PLPC in the LDL of diabetic rats and of SLPC in the LDL of nondiabetic rats all significantly decreased by probucol (all; p < 0.01), with a concomitant decrease of the plasma concentrations of total and HDL cholesterol, and phospholipid, compared with those without probucol. The level of TBARS per protein in LDL increased in diabetic rats, and decreased by probucol (p < 0.01). In conclusion, the oxidative stress is thought to be an important factor to accumulate LPC in LDL, although the paradoxical decrease of SLPC in diabetic LDL suggests a non-oxidative metabolism with a preference for the LPC molecular species. The reducing effect of probucol on LPC may not be solely attributed to its antioxidative effect.

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“…Samples were allowed to clot for 2 h at 4". Part of the serum was immediately treated with polethylene glycol 6000 (100 g/l) at pH 10 for 5 min to precipitate low-density lipoproteins and very-low-density lipoproteins, and then centrifuged (Allen et al 1979). The supernatant fraction was used for estimation of HDL-cholesterol, and untreated serum was used for estimation of total cholesterol.…”

Section: Total Cholesterol and Hdl-cholesterolmentioning

confidence: 99%

A fraction derived from brewer's yeast inhibits cholesterol synthesis by rat liver preparations in vitro

1991

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Brewer's yeast was grown on a defined medium containing tracer51Cr with or without added chromium. The two batches of yeast contained 10 μg/g (high-Cr) or 80 ng/g (low-Cr). Extracts were prepared and fractionated. A third batch of yeast (third batch) was grown with added Cr, and fractionated. Rats were reared on either rat cubes (normal diet) or on a low-Cr diet (low-Cr), or on rat cubes with added cholestyramine (cholestyramine diet). Preparations of rat liver, both cell-free and intact hepatocytes, incorporated acetate-carbon into fatty acids and cholesterol. These processes were inhibited by a yeast fraction containing small, neutral, water-soluble compounds. The degree of inhibition was the same whether the liver came from normal rats or rats fed on the low-Cr diet. Similarly the inhibitory effect was found with identical amounts of extracts from low- or high-Cr yeasts. Therefore, Cr compounds do not appear to account for the inhibitory effects of brewer's yeast. Use of other substrates indicated that the site of inhibition of sterol synthesis was apparently between acetyl-CoA and mevalonate. One inhibitory substance was isolated from yeast and was found to be nicotinamide riboside. This may have been produced from NAD(P) during the preparation of yeast extracts, and it may be produced from dietary yeast supplements during digestion in vivo. Nicotinamide riboside may be partly responsible for the reported effects of yeast supplements on plasma lipids in humans.

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An enzymic and centrifugal method for estimating high-density lipoprotein cholesterol.

Cited by 64 publications

References 0 publications

Severe hyperlipoproteinemia in congenital nephrotic syndrome of the Finnish type: effect of dialysis and kidney transplantation

Severe hyperlipoproteinemia in congenital nephrotic syndrome of the Finnish type: effect of dialysis and kidney transplantation

Lysophosphatidylcholine molecular species in low density lipoprotein and high density lipoprotein in alloxan-induced diabetic rats: effect of probucol

A fraction derived from brewer's yeast inhibits cholesterol synthesis by rat liver preparations in vitro

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