An epidermal stem cells niche microenvironment created by engineered human amniotic membrane

Ji, Shizhao; Xiao, Shichu; Luo, Pan; Huang, Guofeng; Wang, Guangyi; Zhu, Shan; Wu, Minjuan; Xia, Zhaofan

doi:10.1016/j.biomaterials.2011.06.076

Cited by 36 publications

(38 citation statements)

References 32 publications

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“…9,23 In today's clinical procedures, multiple autologous flaps or mucosa grafts are used for substitution urethroplasty. But the limited amount of autologous tissue and trauma at donor sites restrict the application of the surgical option.…”

Section: Discussionmentioning

confidence: 99%

Epithelial-Differentiated Adipose-Derived Stem Cells Seeded Bladder Acellular Matrix Grafts for Urethral Reconstruction: An Animal Model

Xie

et al. 2014

Tissue Engineering Part A

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The limited amount of available epithelial tissue is considered a main cause of the high rate of urethral reconstruction failures. The aim of this study was to investigate whether epithelial-differentiated rabbit adiposederived stem cells (Epith-rASCs) could play a role of epithelium in vivo functionally and be a potential substitute of urothelium. Substitution urethroplasty was performed to repair an anterior urethral defect in male New Zealand rabbits using Epith-rASCs seeded bladder acellular matrix grafts (BAMGs) after 5-bromo-2¢-deoxyuridine (BrdU) labeling, based on the in vitro epithelial induction system we previously described. Urethroplasty with cell-free BAMGs and with undifferentiated rASCs (Und-rASCs) seeded BAMGs were performed as controls. After surgery, a notable amelioration of graft contracture and recovery of urethral continuity were observed in the Epith-rASCs/BAMG group by retrograde urethrograms and macroscopic inspection. Immunofluorescence revealed that the BrdU-labeled Epith-rASCs/Und-rASCs colocalized with cytokeratin 13 or myosin. Consistent with the results of western blotting, at early postimplantation stage, the continuous epithelial layer with local multilayered structure was observed in the Epith-rASCs/BAMG group, whereas no significant growth and local monolayer growth profile of epithelial cells were observed in the BAMG and Und-rASCs/BAMG group, respectively. The results showed that Epith-rASCs could serve as a potential substitute of urothelium for urethral tissue engineering and be available to prevent lumen contracture and subsequent complications including recurrent stricture.

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Section: Discussionmentioning

confidence: 99%

Epithelial-Differentiated Adipose-Derived Stem Cells Seeded Bladder Acellular Matrix Grafts for Urethral Reconstruction: An Animal Model

Xie

et al. 2014

Tissue Engineering Part A

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“…It was found in our study that 8-h Dispase II digestion was able to separate the epidermis from the dermis completely, and the epidermal sheet obtained not only preserved an intact epidermis without any dermal or BM structures, but also maintained high tissue viability. P63 is a nuclear transcription factor expressed in undifferentiated proliferating cells and is critically important for skin and appendage development and maintenance [17], [18]. The results of our immunohistochemical staining showed that the percentage of P63 positive keratinocytes in the epidermal sheets was similar with normal skin, indicating that the keratinocytes maintained good proliferating ability.…”

Section: Discussionmentioning

confidence: 58%

“…Cell proliferating ability of the epidermal sheet was detected by immunohistochemical staining of P63 [17], [18]. Briefly, after being deparaffinized, rinsed and blocked with 4% bovine serum, the sections were incubated with monoclonal antibodies against P63 (1∶200, sc-8431, Santa Cruz, USA) at 4°C overnight.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Dermal Substitute in a Novel Co-Transplantation Model with Autologous Epidermal Sheet

Huang

Luo

et al. 2012

PLoS ONE

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The development of more and more new dermal substitutes requires a reliable and effective animal model to evaluate their safety and efficacy. In this study we constructed a novel animal model using co-transplantation of autologous epidermal sheets with dermal substitutes to repair full-thickness skin defects. Autologous epidermal sheets were obtained by digesting the basement membrane (BM) and dermal components from rat split-thickness skins in Dispase II solution (1.2 u/ml) at 4°C for 8, 10 and 12 h. H&E, immunohistochemical and live/dead staining showed that the epidermal sheet preserved an intact epidermis without any BM or dermal components, and a high percentage of viable cells (92.10±4.19%) and P63 positive cells (67.43±4.21%) under an optimized condition. Porcine acellular dermal matrixes were co-transplanted with the autologous epidermal sheets to repair full-thickness skin defects in Sprague-Dawley rats. The epidermal sheets survived and completely re-covered the wounds within 3 weeks. Histological staining showed that the newly formed stratified epidermis attached directly onto the dermal matrix. Inflammatory cell infiltration and vascularization of the dermal matrix were not significantly different from those in the subcutaneous implantation model. Collagen IV and laminin distributed continuously at the epidermis and dermal matrix junction 4 weeks after transplantation. Transmission electron microscopy further confirmed the presence of continuous lamina densa and hemidesmosome structures. This novel animal model can be used not only to observe the biocompatibility of dermal substitutes, but also to evaluate their effects on new epidermis and BM formation. Therefore, it is a simple and reliable model for evaluating the safety and efficacy of dermal substitutes.

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“…Four weeks after transplantation, papilla-like structures were observed, and collagen fibers were well and regularly arranged in the newly formed dermal layer. In conclusion, the mAM as a novel natural microcarrier possesses an intact basement membrane structure and bioactivities [8].…”

Section: Amniotic Membranementioning

confidence: 90%

Biomaterials for Tendon/Ligament and Skin Regeneration

Cheng¹

2018

Biomaterials in Regenerative Medicine

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Tendon/ligament injury or skin injuries due to diseases, trauma, and surgery are common. Timely functional repair and tissue regeneration is a key to improve the quality of life of the patient while reducing health care cost. Tendon/ligament/skin is also enriched in a common extracellular matrix (ECM), collagen I, III, and elastin. Tissue engineering and regenerative medicine, the combination of (stem) cells, growth factors, and biomaterial scaffolds, is an emergent field, which has attracted substantial attention over the years. Biomaterials are considered the foundation of regenerative medicine. A key to find a new solution to tendon/ligament/skin healing is to synthesize new functional biomaterials, which have better biomechanical properties, biodegradability, and cell supporting properties. This chapter will review existing FDA-approved biomaterial-based therapy, as well as those in development.

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An epidermal stem cells niche microenvironment created by engineered human amniotic membrane

Cited by 36 publications

References 32 publications

Epithelial-Differentiated Adipose-Derived Stem Cells Seeded Bladder Acellular Matrix Grafts for Urethral Reconstruction: An Animal Model

Epithelial-Differentiated Adipose-Derived Stem Cells Seeded Bladder Acellular Matrix Grafts for Urethral Reconstruction: An Animal Model

Evaluation of Dermal Substitute in a Novel Co-Transplantation Model with Autologous Epidermal Sheet

Biomaterials for Tendon/Ligament and Skin Regeneration

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