An Escherichia coli clone carrying the adhesin-encoding afa operon is involved in both diarrhoea and cystitis in twins

Germani, Y; Begaud, E.; Duval, Philippe; Bouguénec, Chantal Le

doi:10.1016/s0035-9203(97)90031-6

Cited by 18 publications

(11 citation statements)

References 6 publications

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“…Despite their different origins, both strains appear very similar at the molecular level. The observation that E. coli C1845, isolated from a child with diarrhea, is very close to uropathogenic isolates is in agreement with the hypothesis developed by Germani et al (19) that a single Afa/Dr strain can be responsible for diarrhea or UTI and/or with the hypothesis that isolation of Afa/Dr DAEC strains from patients with diarrhea may be related to the presence of uropathogenic E. coli in the colon without a causative link to the disease state (17).…”

Section: Discussionsupporting

confidence: 88%

Representational Difference Analysis between Afa/Dr Diffusely Adhering Escherichia coli and Nonpathogenic E. coli K-12

et al. 2002

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Diffusely adhering Escherichia coli strains harboring Afa/Dr adhesins (Afa/Dr DAEC) have been associated with diarrhea and urinary tract infections (UTIs). The present work is the first extensive molecular study of a Afa/Dr DAEC strain using the representational difference analysis technique. We have searched for DNA sequences present in strain C1845, recovered from a diarrheagenic child, but absent from a nonpathogenic K-12 strain. Strain C1845 harbors part of a pathogenicity island (PAI CFT073 ) and several iron transport systems found in other E. coli pathovars. We did not find genes encoding factors known to subvert host cell proteins, such as type III secretion system or effector proteins. Several C1845-specific sequences are homologous to putative virulence genes or show no homology with known sequences, and we have analyzed their distribution among Afa/Dr and non-Afa/Dr clinical isolates and among strains from the E. coli Reference Collection. Three C1845-specific sequences (MO30, S109, and S111) have a high prevalence (77 to 80%) among Afa/Dr strains and a low prevalence (12 to 23%) among non-Afa/Dr strains. In addition, our results indicate that strain IH11128, an Afa/Dr DAEC strain recovered from a patient with a UTI, is genetically closely related to strain C1845.Diffusely adhering Escherichia coli (DAEC) strains, which are characterized by their diffuse adherence pattern on cultured epithelial HeLa cells (56), have been recognized as the sixth class of diarrheagenic E. coli and appear as a heterogeneous group (12,41). A subclass of DAEC strains harbors adhesins of the Afa/Dr family (including Afa-I, Afa-III, Dr, Dr-II, and F1845), which recognize the decay-accelerating factor (DAF, or CD55) as a common receptor (43). Afa/Dr DAEC strains are identified in epidemiological studies by hybridization to a specific probe, daaC, which is common to operons encoding Afa/Dr adhesins (5). In addition, a PCR assay to detect Afa/Dr strains has been described recently (35). Afa/Dr DAEC strains have been associated with diarrhea in children (20,22,24,28). However, some studies have reported that Afa/Dr DAEC strains are found equally in children with and without diarrhea (1,17). Discrepancies between epidemiological studies could be explained in part by age-dependent susceptibility (37). Unlike other diarrheagenic pathovars of E. coli, Afa/Dr DAEC strains are also important in urinary tract infections (UTI) (43).The alterations induced by wild-type Afa/Dr DAEC strains on polarized host epithelial cells have been studied extensively, but these strains remain poorly characterized at the molecular level. Infection of polarized cultured human intestinal cells by Afa/Dr DAEC strain C1845, isolated from a patient with diarrhea, or IH11128, recovered from a patient with UTI, is followed by elongation of brush border microvilli resulting from rearrangement of cytoskeleton proteins (4, 47), alteration of tight-junction-associated proteins (46), and impairment of several brush border-associated enzymatic activities (45, 47). T...

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Section: Discussionsupporting

confidence: 88%

Representational Difference Analysis between Afa/Dr Diffusely Adhering Escherichia coli and Nonpathogenic E. coli K-12

et al. 2002

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“…An AfaE-I-producing clone lacking other virulence factors has been reported to be the causative agent of both diarrhea and cystitis (16). We found that the afaE1 subtype was one of the most frequent in isolates from patients with pyelonephritis, sepsis, and diarrhea.…”

Section: Discussionmentioning

confidence: 70%

“…Their distribution among afa-positive diarrheagenic strains is unknown. Interestingly, the same afaE1-positive strain has been implicated as the causative agent of consecutive diarrhea and cystitis in an individual child (16).…”

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confidence: 99%

Characterization of AfaE Adhesins Produced by Extraintestinal and Intestinal Human Escherichia coli Isolates: PCR Assays for Detection of Afa Adhesins That Do or Do Not Recognize Dr Blood Group Antigens

Bouguénec¹,

Lalioui²,

Merle³

et al. 2001

J Clin Microbiol

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adhesins) on the human decay-accelerating factor (DAF) as a receptor, and others (Afa/Dr؊ adhesins) do not. Thus, the afa operons detected in this study were characterized by subtyping the afaE gene using specific PCRs. In addition, the DAF-binding capacities of as-yet-uncharacterized AfaE adhesins were tested by various cellular approaches. The afaE8 subtype (Afa/Dr ؊ adhesin) was found to predominate in afa-positive isolates from sepsis patients (75%); it was frequent in afa-positive pyelonephritis E. coli (55.5%) and absent from diarrhea-associated strains. In contrast, Afa/Dr ؉ strains (regardless of the afaE subtype) were associated with both diarrhea (100%) and extraintestinal infections (44 and 25% in afa-positive pyelonephritis and sepsis strains, respectively). These data suggest that there is an association between the subtype of AfaE adhesin and the physiological site of the infection caused by afa-positive strains.

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“…French groups have continued to investigate these Afa-positive DAEC and have provided information regarding their molecular bacteriology (2,(14)(15)(16)(31)(32)(33)(34). Furthermore, Bétis et al (3,4) found that two DAEC strains induced interleukin-8 (IL-8) in human colonic epithelial cell line, T84.…”

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confidence: 99%

Interleukin‐8 Secretion by Epithelial Cells Infected with Diffusely Adherent Escherichia coli Possessing Afa Adhesin‐Coding Genes

Arikawa

Meraz

Nishikawa

et al. 2005

Microbiology and Immunology

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Escherichia coli is the predominant facultative anaerobe of the normal colon flora. However, particular clones can cause sepsis, urinary tract infection, and diarrheal disease. The diarrheagenic E. coli (DEC) are classified according to pathogenicity as enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC), enterohemorrhagic E. coli (EHEC), and enteroaggregative E. coli (EAggEC) (36).Colonization of the human intestine is an essential part of the infection process. Diffusely adherent E. coli (DAEC) can bind to HEp-2 cells (46) and represent a putative sixth DEC class. Their role in diarrheal disease, however, has been controversial (11,18,25). Several groups have used age-dependent susceptibility to explain epidemiological discrepancies, since a positive association with diarrhea was found when study populations were age-stratified (1,18,20,35,47).Clinical microbiologists have to hesitate to classify the DAEC isolates as causative agents. DAEC probably comprise a heterogeneous group of organisms with variable enteropathogenicity (45). Measuring diffuse adhesion alone is insufficient to evaluate the diarrheagenicity of strains and other distinguishing characteristics are needed. About 90% of uropathogenic E. coli colonize on urinary tract epithelial cells by recog-

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An Escherichia coli clone carrying the adhesin-encoding afa operon is involved in both diarrhoea and cystitis in twins

Cited by 18 publications

References 6 publications

Representational Difference Analysis between Afa/Dr Diffusely Adhering Escherichia coli and Nonpathogenic E. coli K-12

Representational Difference Analysis between Afa/Dr Diffusely Adhering Escherichia coli and Nonpathogenic E. coli K-12

Characterization of AfaE Adhesins Produced by Extraintestinal and Intestinal Human Escherichia coli Isolates: PCR Assays for Detection of Afa Adhesins That Do or Do Not Recognize Dr Blood Group Antigens

Interleukin‐8 Secretion by Epithelial Cells Infected with Diffusely Adherent Escherichia coli Possessing Afa Adhesin‐Coding Genes

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