An Esterase with Increased Acetone Tolerance from Bacillus subtilis E9 over Expressed in E. coli BL21 Using pTac Bs-est Vector

Heterologous expression, molecular studies and biochemical characterization of a novel alkaline esterase gene fromBacillus thuringiensisfor detergent industry

“…[45][46][47] Optimization of foreign gene expression is also reported in previous studies. [48][49][50] Soumya et al 51 reported a cloned esterase gene from Bacillus subtilis E9 into E. coli using pTac Bs-est vector, the overexpression of which was attained by IPTG induction by optimizing the time required for over expression of the gene and culture media. Similarly, Fan et al 52 optimized different parameters for the maximum production of a recombinant esterase enzyme from E. coli and reported maximum production with LB medium having pH 5.5, with 5 h pre-induction period and 32 h aer induction period at 23 °C.…”