An evaluation of the effect of a gastric ischemia–reperfusion model with laser Doppler blood perfusion imaging

Abstract

The objectives of this study are to establish a gastric ischemia-reperfusion model and test it using the laser Doppler blood perfusion imaging (LDPI) method and to evaluate the role of the LDPI technique in the measure of gastric blood circulation. The right gastric artery of the rat was clamped for 30 min and then reperfused. The LDPI was used to display the blood circulation of the whole gastric surface during this process. The blood flow perfusion image of the gastric surface was displayed clearly. After the right gastric artery was clamped, the blood flow perfusion on the gastric surface decreased very significantly until the clamp was loosened. Following reperfusion, the blood flow suddenly increased. Within the first 10 min, the blood-flow perfusion exceeded the level before the clamping and then gradually became smooth and steady. The right gastric artery is a main pathway for gastric blood supply. LDPI can display successfully the blood circulation state of the stomach and the course of ischemia-reperfusion of a large area with an image.

The Time Course Effects of Electroacupuncture on Promoting Skeletal Muscle Regeneration and Inhibiting Excessive Fibrosis after Contusion in Rabbits

“…LDPI can detect microcirculation status after a direct stress reaction and has been widely used in clinical diagnosis, evaluation, and research associated with microcirculation such as acupuncture [35] and skeletal muscle microcirculation [36]. We measured the number of perfusion units in this study: PU = CMBC (the concentration of measuring the volume inside the blood cells) × V (the average velocity of blood cells).…”

Evaluation of liver function and electroacupuncture efficacy of animals with alcoholic liver injury by the novel imaging methods

“…Imaging methods to evaluate HMP by directly monitoring the overall HM remain lacking. Our laboratory applied laser Doppler blood flow imaging and LSPI technology for the first time to display perfusion over the entire surface of an animal stomach 28 . On this basis, this study established a liver LSPI method for the intuitive and dynamic observation of the HMP in livers with alcoholic injury.…”

Investigation of Hepatic Blood Perfusion by Laser Speckle Imaging and Changes of Hepatic Vasoactive Substances in Mice after Electroacupuncture

“…It is reported that acupuncture treatment can improve hemorheology and vasoactive substances, such as thromboxane A 2 (TXA 2 ), prostaglandin I 2 (PGI 2 ), endothelin (ET), and atrial natriuretic factor (ANF) [ 5 ]. Zhang et al [ 6 ] reported that blood supply in the stomach of ischemia-reperfusion is improved and promoted by EA stimulation. Therefore, the increase of visceral blood perfusion (BP) is an important effect of acupuncture and a basis in the acupuncture treatment for visceral diseases [ 7 , 8 ].…”