An Evaluation on the Genetic Profiling and Microbial Detection in Prawns

Abstract

The shrimps of India belong to three major families namely: Pedacidac, Palacmonidae and Sergestidae of the decapods group. They are especially rich in niacin, essential for a healthy skin and for the release of energy in the body and vitamin B complex needed for metabolic processes. The study aims at isolating the gut microorganisms such as vibriocholerae and Staphylococcus aureus from P. monodon and A. indicus .Vibrio were isolated by streak plate methods and staphylococcus were isolated by spread plate methods. There microorganisms can cause several diseases to man and including food poisoning .Staphylococcus aureus cause diarrhoea, increase dehydration, etc., while vibriocholerae causes cholera, renal failure, cramps, hypertension , sunken eyes, etc. CTAB extraction method was used for the isolation of genomic DNA. The isolated DNA samples were subjected to agrose gel electrophoresis .The buffer used here was 1xTAE and the DNA bands were observed in UV transilluminator. Spectrophotometric readings of the isolated DNA samples were taken at 260 and 280 nm and these readings were used to estimate the quantity and quality of DNA samples isolated. Prawns are excellent source of protein, a good source of omega 3 fatty acids and a great way to get iron, zinc and vitamin E