An evaluation study of trace element content in colorectal liver metastases and surrounding normal livers by X-ray fluorescence

Gurusamy, Kurinchi Selvan; Farquharson, Michael J.; Craig, Clare; Davidson, Brian R

doi:10.1007/s10534-007-9126-3

Cited by 15 publications

(16 citation statements)

References 26 publications

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“…Zn homeostasis is coordinated through Zn transporter proteins (ZnT), that include ZIP group increases the intracellular level and cation diffusion facilitator (CDF), and metallothioneines. Gurusamy et al (2008) explain the low levels of Zn found in liver metastases in comparison with normal adjacent tissues as a variation in the levels of Zn transporters and a possible increase in the utilization of Zn by the fast growing tumour tissues. An irregular distribution of Zn in tumours might explain these results.…”

Section: Statistical Comparison Of Datamentioning

confidence: 95%

Elemental fingerprinting of tumorous and adjacent non-tumorous tissues from patients with colorectal cancer using ICP-MS, ICP-OES and chemometric analysis

et al. 2009

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Tumorous and adjacent non-tumorous paired biopsies from 38 patients with colorectal cancer were analyzed by inductively coupled plasma mass spectrometry and inductively coupled plasma optical emission spectrometry after low-volume microwave digestion. 18 elements were investigated: Ag, Al, Ca, Cd, Co, Cr, Cu, Fe, K, Mg, Mn, Mo, Ni, P, Pb, S, Se and Zn. Different chemometric tools were used for data evaluation: Wilcoxon signed rank test, Hieratical clustering analysis, principal component analysis (PCA) and linear discriminant analysis (LDA). With the exception of Al, tumours were observed to have significantly more elevated concentrations of essential elements as compared to non-tumours. On the contrary, elements considered potentially carcinogenic such as Cr, Ni, Mo or Co do not display significant differences. When PCA was applied, different components were obtained for tumorous and non-tumorous tissues. When LDA was applied for the elements studied (including essential and non-essential elements) about 90% of cases were correctly classified.

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Section: Statistical Comparison Of Datamentioning

confidence: 95%

Elemental fingerprinting of tumorous and adjacent non-tumorous tissues from patients with colorectal cancer using ICP-MS, ICP-OES and chemometric analysis

et al. 2009

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“…[7][8][9][10][11] A wide range of applications involves analyses of various types of biomedical specimens including soft tissues, [12][13][14] bones, [15][16][17] and teeth. [18][19][20] The XRF analysis was previously applied to study samples of different body organs such as brain, [8,12,21] prostate, [22] breast, [23] lung, [12] liver, [14,24] heart, [12] or kidneys. [12] It is worth pointing out that multiple physical characteristic of a sample influences the interactions of X-rays with the atoms present in the material analyzed.…”

Section: Introductionmentioning

confidence: 99%

Sample thickness considerations for quantitative X‐ray fluorescence analysis of the soft and skeletal tissues of the human body – theoretical evaluation and experimental validation

Szczerbowska-Boruchowska

2012

X-Ray Spectrometry

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Sample thickness considerations for quantitative X-ray fluorescence analysis of the soft and skeletal tissues of the human bodytheoretical evaluation and experimental validation Magdalena Szczerbowska-Boruchowska* A good estimation of the matrix composition and the areal mass of the sample is critical for quantitative X-ray fluorescence (XRF) analysis. Integrated aspects of the XRF quantitative analysis of various human body organs are presented. Special emphasis is placed on the determination of the sample thicknesses at which the specimen can be regarded as thin, thick, or intermediate thickness depending on the element under consideration. Moreover, a method for a fully quantitative analysis allowing the determination of the masses per unit area of chemical elements in thin, thick, and intermediate thickness samples is discussed. It was found that for an incident beam of 17 keV energy, a 15 mm thick sample is of intermediate thickness for all elements between P and Ca and becomes thin from Fe for most human body tissues in a natural form. Dried samples of soft tissues excluding these of low water content can be regarded as thin for all elements from phosphorus to strontium. The use of thin sample approach in quantification of intermediate thickness specimen may result in about 30-45% discrepancy in areal mass (weight fraction) of phosphorus, 20-35% of sulfur, 15-25% of chlorine, 8-15% of potassium, and 5-10% of calcium. Theoretical evaluations presented in the work are verified experimentally. The analysis of human brain samples (white and gray matter) and bovine liver (National Institute of Standards and Technology standard reference materials 1577b) confirms high accuracy of the XRF quantification on the basis of the described procedures.

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“…Iron, copper, and zinc are lower in colorectal liver metastases than in normal surrounding liver (Gurusamy et al 2008;Farquharson et al 2009). The transcription levels of the main zinc efflux transporter ZnT1 is consistently low in colorectal liver metastases compared to normal surrounding liver (submitted for publication).…”

Section: Introductionmentioning

confidence: 99%

Influence of zinc and zinc chelator on HT-29 colorectal cell line

et al. 2010

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Trace elements are involved in many key pathways involving cell cycle control. The influence of zinc and zinc chelator (TPEN) on transcription levels of the main zinc transporters (ZnT1 and ZIP1) in the HT-29 colorectal cell line has not been reported. Proliferation of HT-29 cells was measured using the methylene blue assay after exposure to zinc (two concentrations), TPEN (two concentrations), or a combination of zinc and TPEN (simultaneously and sequentially) for 4 h, 8 h, and 24 h. The transcription levels of ZnT1, ZIP1, vascular endothelial growth factor (VEGF), and caspase-3 were determined using reverse transcriptase real-time polymerase chain reaction (RT-PCR) after exposure of cells to zinc and TPEN. The zinc content in the substrate (medium used for culture) was determined using atomic absorption spectrometry. TPEN decreased cellular proliferation causing complete cell death by 8 h. Zinc had a protective effect against short periods of exposure to TPEN. There was no correlation between the transcripts of main zinc transporters and the zinc content in the substrate. The zinc content in the substrate remained constant after varying periods of cell culture. TPEN decreased the transcript levels of caspase-3 and VEGF, which are surrogate markers for apoptosis and angiogenesis. Zinc chelation of HT-29 cells causes cell death. Zinc appears to be protective for short periods of exposure to TPEN but has no protective effect on prolonged exposure. HT-29 cells are not able to counteract the effect of intracellular chelation of zinc by altering zinc transport. Further research into the mechanisms of these findings is necessary and may lead to novel therapeutic options.

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An evaluation study of trace element content in colorectal liver metastases and surrounding normal livers by X-ray fluorescence

Abstract: Iron, copper, and zinc are lower in colorectal liver metastases than normal liver. An investigation into the pathways underlying these differences may provide a new understanding of cancer development and possible novel therapeutic targets.

Cited by 15 publications

References 26 publications

Elemental fingerprinting of tumorous and adjacent non-tumorous tissues from patients with colorectal cancer using ICP-MS, ICP-OES and chemometric analysis

Elemental fingerprinting of tumorous and adjacent non-tumorous tissues from patients with colorectal cancer using ICP-MS, ICP-OES and chemometric analysis

Sample thickness considerations for quantitative X‐ray fluorescence analysis of the soft and skeletal tissues of the human body – theoretical evaluation and experimental validation

Influence of zinc and zinc chelator on HT-29 colorectal cell line

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