An exonuclease I-based label-free fluorometric aptasensor for adenosine triphosphate (ATP) detection with a wide concentration range

Wei, Yanli; Chen, Yanxia; Li, Huanhuan; Shuang, Shaomin; Dong, Chuan; Wang, Gufeng

doi:10.1016/j.bios.2014.07.064

Cited by 85 publications

(41 citation statements)

References 24 publications

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“…BBR is known to interact with DNA and to have an effect on telomerase activity, 59,60 and it could cause a fluorescence increase of BBR when binding to G4s. 61,62 Then the fluorescence intensities of the H22-BBR complex were collected in the presence of increasing concentrations of Zn 2+ . As shown in Fig.…”

Section: Principle Of Operationmentioning

confidence: 99%

Label-free Detection of Zn2+ Based on G-quadruplex

et al. 2015

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Herein, we developed a sensing strategy for the label-free detection of Zn 2+ based on G-quadruplex. In the absence of Zn 2+ , there was a fluorescence enhancement of thioflavin T by interaction with human telomere sequence. On the addition of Zn 2+ , Zn 2+ induced a more compact G-quadruplex structure to release thioflavin T, resulting in a fluorescence decrease. This simple "mix-then-detect" method gave the detection limit of 0.91 μM with linear dynamic ranges from 0 to 10 μM. Because it does not require the use of expensive and unstable DNAzyme systems, or need synthesis and modification of nanomaterials, this label-free biosensor is simple, fast, cost-effective and applicable for real samples taken from lake water.

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Section: Principle Of Operationmentioning

confidence: 99%

Label-free Detection of Zn2+ Based on G-quadruplex

et al. 2015

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“…Compared with antibodies, aptamers with higher affinity toward targets were used in this work. Aptamer have attracted substantial attention as recognition elements in bio-detections, owing to their easy preparation in batch by PCR, high stability and selectivity, facile modification capabilities over antibodies (Jiang et al, 2015;Wei et al, 2015). In this work, two aptamers respectively for CAP and PCB72, were simultaneously immobilized on magnetic gold nanoparticles (MGPs) to synthesis the capture probes to construct the electrochemical aptasensor without more complex separation and washing steps.…”

Section: Introductionmentioning

confidence: 99%

A “signal-on’’ aptasensor for simultaneous detection of chloramphenicol and polychlorinated biphenyls using multi-metal ions encoded nanospherical brushes as tracers

Yan

Gan

et al. 2015

Biosensors and Bioelectronics

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“…Adenosine triphosphate (ATP), as cellular energy currency in living cells, is one of the most important small-molecule signaling agents [1,2,3,4,5]. It is an indicator for cell viability and cell injury and plays an important role in many biological processes such as the regulation of cellular metabolism and biochemical pathways in cell physiology.…”

Section: Introductionmentioning

confidence: 99%

Visual and Plasmon Resonance Absorption Sensor for Adenosine Triphosphate Based on the High Affinity between Phosphate and Zr(IV)

Liu

Zhang

et al. 2016

Sensors

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Zr(IV) can form phosphate and Zr(IV) (–PO32−–Zr4+–) complex owing to the high affinity between Zr(IV) with phosphate. Zr(IV) can induce the aggregation of gold nanoparticles (AuNPs), while adenosine triphosphate(ATP) can prevent Zr(IV)-induced aggregation of AuNPs. Herein, a visual and plasmon resonance absorption (PRA)sensor for ATP have been developed using AuNPs based on the high affinity between Zr(IV)with ATP. AuNPs get aggregated in the presence of certain concentrations of Zr(IV). After the addition of ATP, ATP reacts with Zr(IV) and prevents AuNPs from aggregation, enabling the detection of ATP. Because of the fast interaction of ATP with Zr(IV), ATP can be detected with a detection limit of 0.5 μM within 2 min by the naked eye. Moreover, ATP can be detected by the PRA technique with higher sensitivity. The A520nm/A650nm values in PRA spectra increase linearly with the concentrations of ATP from 0.1 μM to 15 μM (r = 0.9945) with a detection limit of 28 nM. The proposed visual and PRA sensor exhibit good selectivity against adenosine, adenosine monophosphate, guanosine triphosphate, cytidine triphosphate and uridine triphosphate. The recoveries for the analysis of ATP in synthetic samples range from 95.3% to 102.0%. Therefore, the proposed novel sensor for ATP is promising for real-time or on-site detection of ATP.

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An exonuclease I-based label-free fluorometric aptasensor for adenosine triphosphate (ATP) detection with a wide concentration range

Cited by 85 publications

References 24 publications

Label-free Detection of Zn2+ Based on G-quadruplex

Label-free Detection of Zn2+ Based on G-quadruplex

A “signal-on’’ aptasensor for simultaneous detection of chloramphenicol and polychlorinated biphenyls using multi-metal ions encoded nanospherical brushes as tracers

Visual and Plasmon Resonance Absorption Sensor for Adenosine Triphosphate Based on the High Affinity between Phosphate and Zr(IV)

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