An experimental sclerosing encapsulating peritonitis model in mice

Ishíi, Yasuo; Sawada, Tokihiko; Shimizu, Akira; Tojimbara, Tamotsu; Nakajima, Ichiro; Fuchinoue, Shohei; Teraoka, Satoshi

doi:10.1093/ndt/16.6.1262

Cited by 81 publications

(77 citation statements)

References 11 publications

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“…[16,17] Junor and colleagues first described the role of gluconate and alcohol in the pathogenesis of peritoneal fibrosis in 1985. [10] They reported the development of Effects of Low-Dose ESA on Peritoneal Fibrosis 571 clinical ESP when peritoneal catheter gets sterilized by chlorhexidine gluconate in alcohol. Upon this finding, Ishii and colleagues showed the development of peritoneal fibrosis in test animals by using 0.1% chlorhexidine gluconate and 15% alcohol dissolved in saline.…”

Section: Discussionmentioning

confidence: 99%

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The Effects of Low-Dose Erythropoiesis-Stimulating Agents on Peritoneal Fibrosis Induced by Chemical Peritonitis and on Peritoneal Tissue MMP–2 and TIMP–2 Levels in Rats

et al. 2009

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Aim. The objective of the present study was to investigate the effect of low-dose erytropoesis-stimulating agents (ESA) on the development of peritoneal fibrosis in chlorhexidine gluconateinduced peritoneal sclerosing rats and to assess the peritoneal tissue levels of MMP-2 and TIMP-2, which may be regarded as factors in the development of peritoneal fibrosis. Subjects and methods. Twenty-four Wistar albino rats were divided into three groups. The control group received 0.9% saline (3 ml/d) intraperitoneally, the CH group received 3 ml daily injections of 0.1% chlorhexidine gluconate (CH) intraperitoneally, and the CH+ESA group received 3 ml daily injections of 0.1% CH intraperitoneally and epoetin beta (3 × 20 IU/kg/week) subcutaneously. On the twenth-first day, rats were sacrificed, and parietal peritoneum samples were obtained from the left anterior abdominal wall. Pathological samples were examined using Hematoxyline & Eosin (HE) stains. The thickness, vascular proliferation, and inflammation were determined by light microscopy. MMP-2 and TIMP-2 were studied immunohistochemically by monoclonal antibody staining. Results. Inflammation, vascular proliferation, and fibrotic area percentages were not statistically significant between groups. Histopathologically control, CH, CH+ESA groups peritoneal thickness were 8.02 ± 2.89, 146.74 ± 26.1, and 48.12 ± 16.8 micrometers, respectively. The decrease in thickness of parietal peritoneum in CH+ESA group was statistically significant when compared to CH. Immunohistochemically, interferon was shown to decrease MMP-2 expression on parietal peritoneum than group CH, but has no effect on TIMP-2. Discussion. Lowdose ESA histopatologically reduces peritoneal fibrosis induced by chlorhexidine gluconate. However, from dosage and duration points of view, we need extended clinical and experimental studies.

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Section: Discussionmentioning

confidence: 99%

“…The duration of the study was 12 days. To induce peritoneal fibrosis, we used a model described by Ishii and et al [10] that composed of 0.1% CH and 15% ethyl alcohol dissolved in saline prepared in aseptic era. Epoetin beta (Neorecormon, F. Hoffmann-La Roche Ltd., Basel, Switzerland) was used as 1000 IU/0.3 ml.…”

Section: Methodsmentioning

confidence: 99%

The Effects of Low-Dose Erythropoiesis-Stimulating Agents on Peritoneal Fibrosis Induced by Chemical Peritonitis and on Peritoneal Tissue MMP–2 and TIMP–2 Levels in Rats

et al. 2009

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“…6,7 The latter two agents caused peritoneal adhesions that were very similar to those seen in patients with encapsulating peritoneal sclerosis (EPS), a serious complication of long-term PD therapy. Although the mechanism underlying the development of peritoneal fibrosis by CG is unclear, previous reports have shown that persistent chemical irritation by CG could induce tissue damage with inflammation characterized by infiltration of macrophages followed by excessive proliferation of fibroblasts.…”

Section: Introductionmentioning

confidence: 93%

Involvement of Lymphocyte Infiltration in the Progression of Mouse Peritoneal Fibrosis Model

et al. 2012

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Peritoneal fibrosis is a serious complication in patients with severe chronic kidney disease who are undergoing peritoneal dialysis (PD). One of the pathological characteristics of peritoneal fibrosis is the infiltration of macrophages in the thickened submesothelial compact zone. In addition, infiltration of lymphocytes, including T and B lymphocytes, is observed in the fibrotic peritoneum. However, the relationship between lymphocyte infiltration and progression of peritoneal fibrosis remains unclear. In this study, we investigated the role of lymphocytes in the development of peritoneal fibrosis induced by chlorhexidine gluconate (CG) by comparing the histological changes observed in severe combined immunodeficient (SCID) mice (largely lacking functional T and B lymphocytes) with those observed in wild-type (WT) mice. As expected, CG-injected WT mice showed a thickening of the submesothelial compact zone together with massive collagen deposition accompanied by increased numbers of infiltrating macrophages and T and B lymphocytes. In the peritoneum of SCID mice, the submesothelial compact zone was thicker and the number of macrophages and B lymphocytes was significantly higher than that observed in control immunodeficient and WT mice. In contrast, the number of T lymphocytes in the peritoneum of SCID mice was significantly lower than that in the peritoneum of WT mice. These results suggest that T and B lymphocytes modulate the process of peritoneal fibrosis via macrophage infiltration.

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“…The animals were followed for 56 days. All animals in the group that used chlorhexidine and alcohol 18 developed encapsulating peritonitis 5 .…”

Section: Introductionmentioning

confidence: 99%

“…Ishii et al 5 developed a model in mice using intraperitoneal injection of chlorhexidine gluconate 0.1% and 15% ethanol dissolved in saline at a dose of 0.3 ml daily, using buffered saline in the control group. The animals were followed for 56 days.…”

Section: Introductionmentioning

confidence: 99%

The influence of simvastatin in induced peritoneal fibrosis in rats by peritoneal dialysis solution with glucosis 4.25%

et al. 2012

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PURPOSE: To investigate the influence of using simvastatin on the peritoneal fibrosis induced in rats using peritoneal dialysis solution with glucoses 4.25%. METHODS: Prospective controlled study in 20 non-uremic Wistar rats. The animals received a peritoneal infusion of 10 ml/100 g of peritoneal dialysis solution glucose 4.25% on a daily basis. The animals were divided in two groups: experimental and control. The experimental group received simvastatin 4 mg/kg/d, by a gastric tube. The control group did not receive any drug. The follow-up was 21 and 49 days. At the end, one surgical procedure was performed to get histological samples of visceral and parietal peritoneum. The samples were analyzed using Hematoxylin Eosin and Sirius Red, to evaluate the severity of the fibrosis. RESULTS: The analysis showed that the intensity of the fibrosis, the peritoneal thickness and the cell number in experimental and control groups were not statistically significant different in experimental and control groups. CONCLUSION: The simvastatin do not decrease the intensity of fibrosis on the peritoneal membrane that happens on rats on peritoneal dialysis.

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An experimental sclerosing encapsulating peritonitis model in mice

Abstract: We have developed a convenient experimental model of SEP in mice, which may be useful in elucidating the pathogenesis of SEP and in establishing possible treatments.

Cited by 81 publications

References 11 publications

The Effects of Low-Dose Erythropoiesis-Stimulating Agents on Peritoneal Fibrosis Induced by Chemical Peritonitis and on Peritoneal Tissue MMP–2 and TIMP–2 Levels in Rats

The Effects of Low-Dose Erythropoiesis-Stimulating Agents on Peritoneal Fibrosis Induced by Chemical Peritonitis and on Peritoneal Tissue MMP–2 and TIMP–2 Levels in Rats

Involvement of Lymphocyte Infiltration in the Progression of Mouse Peritoneal Fibrosis Model

The influence of simvastatin in induced peritoneal fibrosis in rats by peritoneal dialysis solution with glucosis 4.25%

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