An Experimental Study and a Randomized, Double-Blind, Placebo-Controlled Clinical Trial to Evaluate the Antisecretory Activity of<i>Lactobacillus acidophilus</i>Strain LB Against Nonrotavirus Diarrhea

Moal, Vanessa Liévin-Le; Sarrazin-Davila, Luis E; Servin, Alain L.

doi:10.1542/peds.2006-2930

Cited by 71 publications

(48 citation statements)

References 46 publications

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“…Consistent with the in vitro and in vivo antibacterial activities reported above for live and heat-treated L. acidophilus strain LB against enteroadherent and enteroinvasive bacteria (122,137,147,181,182), two RCTs have demonstrated the therapeutic efficacy of Lacteol in combination with ORS in decreasing the frequency and duration (reduced by at least half) of enterovirulent bacterium-associated acute watery diarrhea in children between the ages of 1 and 4 years (207,327). Moreover, in adults over 16 years of age with chronic bacterium-or parasite-associated diarrhea, a prospective, randomized, multicenter clinical trial has demonstrated that administering Lacteol led to the recovery of consistent stools in 81% of the treated patients (328).…”

Section: Therapeutic Effects Against Various Forms Of Acute Diarrheasupporting

confidence: 73%

“…The secreted autotransporter toxin, Sat, belonging to the subfamily of SPATE toxins (170), expressed by Afa/Dr DAEC strain C1845 (206) promotes an increase of fluid dome formation in Caco-2/TC7 cell monolayers by modifying the transcellular passage of fluids. L. acidophilus LB CFCS treatment of Caco-2 cell monolayers results in the disappearance of the Sat-induced fluid dome formation, indicating a regulatory effect on the intestinal transcellular pathway of fluids (207).…”

Section: Activities Against the Deleterious Effects Induced By Infectmentioning

confidence: 95%

“…In T84 cells, L. casei DN-114 001 abrogates the EPEC strain E2348/69-induced increase in paracellular permeability and rearrangements of ZO-1 in a dose-dependent manner (186). The antagonistic activity of L. acidophilus LB CFCS against the Sat-induced increase in paracellular permeability and formation of fluid-formed domes in enterocyte-like Caco-2/TC7 cell monolayers (207) probably includes an effect at the TJs, since the toxin affects the structural TJ organization (206). Consistent with this hypothesis, it has been observed that L. acidophilus LB culture protects TJs of cultured human intestinal HT-29 cells by counteracting the aspirin-induced delocalization of structural TJ-associated ZO-1 protein (211).…”

Section: Activities Against the Deleterious Effects Induced By Infectmentioning

confidence: 98%

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Anti-Infective Activities of Lactobacillus Strains in the Human Intestinal Microbiota: from Probiotics to Gastrointestinal Anti-Infectious Biotherapeutic Agents

2014

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SUMMARY A vast and diverse array of microbial species displaying great phylogenic, genomic, and metabolic diversity have colonized the gastrointestinal tract. Resident microbes play a beneficial role by regulating the intestinal immune system, stimulating the maturation of host tissues, and playing a variety of roles in nutrition and in host resistance to gastric and enteric bacterial pathogens. The mechanisms by which the resident microbial species combat gastrointestinal pathogens are complex and include competitive metabolic interactions and the production of antimicrobial molecules. The human intestinal microbiota is a source from which Lactobacillus probiotic strains have often been isolated. Only six probiotic Lactobacillus strains isolated from human intestinal microbiota, i.e., L. rhamnosus GG, L. casei Shirota YIT9029, L. casei DN-114 001, L. johnsonii NCC 533, L. acidophilus LB, and L. reuteri DSM 17938, have been well characterized with regard to their potential antimicrobial effects against the major gastric and enteric bacterial pathogens and rotavirus. In this review, we describe the current knowledge concerning the experimental antibacterial activities, including antibiotic-like and cell-regulating activities, and therapeutic effects demonstrated in well-conducted, placebo-controlled, randomized clinical trials of these probiotic Lactobacillus strains. What is known about the antimicrobial activities supported by the molecules secreted by such probiotic Lactobacillus strains suggests that they constitute a promising new source for the development of innovative anti-infectious agents that act luminally and intracellularly in the gastrointestinal tract.

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Section: Therapeutic Effects Against Various Forms Of Acute Diarrheasupporting

confidence: 73%

Section: Activities Against the Deleterious Effects Induced By Infectmentioning

confidence: 95%

Section: Activities Against the Deleterious Effects Induced By Infectmentioning

confidence: 98%

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Anti-Infective Activities of Lactobacillus Strains in the Human Intestinal Microbiota: from Probiotics to Gastrointestinal Anti-Infectious Biotherapeutic Agents

2014

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“…Others have shown that heat-killed L. acidophilus LB plus its culture medium antagonizes the C1845-induced increase in paracellular permeability in intestinal Caco-2/TC7 cells and produces a clinically significant benefit in the management of children with nonrotavirus diarrhea (33). Our study shows that LPS-induced inflammation can be modulated by the use of both live and heat killed LGG.…”

Section: Discussionsupporting

confidence: 50%

Live and Heat-Killed Lactobacillus rhamnosus GG: Effects on Proinflammatory and Anti-Inflammatory Cytokines/Chemokines in Gastrostomy-Fed Infant Rats

et al. 2009

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Lactobacillus rhamnosus GG (LGG), a probiotics, ameliorates intestinal and other organ inflammation in infant rats. The hypothesis is that live and heat-killed LGG have similar effects on decreasing the inflammatory response induced by E. coli lipopolysaccharide (LPS) in the infant rat. Using a gastrostomy-fed rat model, 7-d-old rat pups were gastrostomy fed with or without live LGG (10 8 or 10In a separate experiment, LPS was administered to rat pups with or without live or heat-killed LGG (10. Cytokine/chemokine proteins were determined by ELISA or multiplex assay. Both live and heat-killed LGG decreased LPS-induced cytokine-induced neutrophil chemoattractant-1 (CINC-1) production in liver and plasma (p Ͻ 0.05) and also showed a trend (p ϭ 0.09) in lungs. Live and heat-killed LGG ameliorated LPS-suppressed IL-10 level in lungs (p Ͻ 0.05). Both forms of LGG decreased IL-1b production in liver. There was no difference between low and high doses of live LGG in the production of CINC-1, TNF-␣, and myeloperoxidase (MPO). There was a trend of increase of claudin-1 in both live and heat-killed groups (p ϭ 0.08). In conclusion, both live and heat-killed LGG provided by the enteral route decrease LPS-induced proinflammatory mediators and increase anti-inflammatory mediators.

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“…Combined supplementation of Lactobacillus and Bifidobacterium species may ameliorate symptoms of collagenous colitis 16 . Heat-killed Lactobacillus acidophilus also shortens the recovery time for non-rotavirus diarrhoea in children 17 , and prevents antibiotic-associated diarrhoea in hospitalized patients 18 . Allergies may also be prevented by oral supplementation of Lactobacillus and Bifidobacterium species 19 .…”

Section: Introductionmentioning

confidence: 99%

Effects of a probiotic preparation on the human faecal microflora and biochemical and haematological parameters

Lan

Hsieh²,

Tsai

et al. 2015

ScienceAsia

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ABSTRACT:The aim of this study was to investigate the effects of supplementation of a probiotic preparation PROBIO-C containing Lactobacillus acidophilus, Bifidobacterium longum, L. rhamnosus, and Streptococcus thermophilus on the intestinal microflora of healthy volunteers and the effects on biochemical and haematological measurements. In vitro analyses, which included acid and bile salts tolerance and adhesion tests, were performed first. Probiotic bacteria in PROBIO-C demonstrated adhesion to human intestinal Caco-2 cells. An intervention study was conducted in a single-blind, random, and placebo-controlled trial. Twenty-four healthy volunteers were randomly assigned to one of three treatment groups, one provided with the placebo powder excluding the probiotic, one with 3 g of PROBIO-C with a targeted dose of 1.5 × 10 11 CFU of probiotic bacteria, and one with 6 g of PROBIO-C with a targeted dose of 3 × 10 11 CFU of probiotic bacteria. The experiment comprised three phases: the control period (week 1) during which no supplementation was given; the test period (weeks 2-4) during which volunteers were given either the placebo sample or PROBIO-C each day; the post-test period (week 5) during which no supplementation was given. The faecal samples were collected once every week throughout the study. The abundances of Bifidobacterium and Lactobacillus species increased significantly during the test period in both PROBIO-C supplemented groups. In addition, the probiotic supplementation did not result in any significant change in clinical chemistry and haematological parameters. The results from this pilot study indicated that PROBIO-C is well-tolerated and safe.

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An Experimental Study and a Randomized, Double-Blind, Placebo-Controlled Clinical Trial to Evaluate the Antisecretory Activity ofLactobacillus acidophilusStrain LB Against Nonrotavirus Diarrhea

Abstract: Heat-killed L. acidophilus LB plus its culture medium antagonizes the C1845-induced increase in paracellular permeability in intestinal Caco-2/TC7 cells and produces a clinically significant benefit in the management of children with nonrotavirus, well-established diarrhea.

Cited by 71 publications

References 46 publications

Anti-Infective Activities of Lactobacillus Strains in the Human Intestinal Microbiota: from Probiotics to Gastrointestinal Anti-Infectious Biotherapeutic Agents

Anti-Infective Activities of Lactobacillus Strains in the Human Intestinal Microbiota: from Probiotics to Gastrointestinal Anti-Infectious Biotherapeutic Agents

Live and Heat-Killed Lactobacillus rhamnosus GG: Effects on Proinflammatory and Anti-Inflammatory Cytokines/Chemokines in Gastrostomy-Fed Infant Rats

Effects of a probiotic preparation on the human faecal microflora and biochemical and haematological parameters

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