An Extracellular Substance from the White-Rot Basidiomycete <i>Phanerochaete chrysosporium </i>for Reducing Molecular Oxygen and Ferric Iron

Tanaka, Hiromi; Itakura, Shuji; Hirano, Toshitsugu; Enoki, Akio

doi:10.1515/hfsg.1996.50.6.541

Cited by 28 publications

(20 citation statements)

References 38 publications

(34 reference statements)

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“…99 Em vários trabalhos posteriores o mesmo grupo isolou os metabólitos proteicos extracelulares a partir de culturas de fungos de decomposição branca e parda cultivados em cavacos de madeira. 31,[100][101][102][103][104][105] Os compostos parcialmente purificados foram caracterizados como glicopeptídeos de baixa massa molar (1)(2)(3)(4)(5) 100,102,104 Este modelo oxidativo de baixa massa molar ainda requer mais estudos, uma vez que até o momento não há trabalhos que reportem a produção extracelular de NADH por fungos degradadores de madeira. Além disso, se este modelo for realmente utilizado por estes fungos, é provável que algum outro composto atue como doador de elétron ao invés de NADH.…”

Section: Peptídeosunclassified

Relevância de compostos de baixa massa molar produzidos por fungos e envolvidos na biodegradação da madeira

Arantes¹,

Milagres²

2009

Quím. Nova

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Recebido em 16/7/08; aceito em 27/1/09; publicado na web em 3/7/09 RELEVANCE OF LOW MOLECULAR WEIGHT COMPOUNDS PRODUCED BY FUNGI AND INVOLVED IN WOOD BIODEGRADATION. Over the last decade, evidences have been shown that the wood biodegradation by fungi is not only a result of the action of their enzymatic machinery but also of various low molecular weight non-enzymatic compounds, especially in fungi that promote brown and white decay, which in nature are the major wood decaying microorganisms. The present review focuses on the recent theories involving these low molecular weight compounds that act direct or synergistically with lignocellulolytic enzymes to attack the wood main macromolecular constituents, their relevance as potential degradative systems, in the overall wood biodegradation, and also outlines their potential biotechnological applications.Keywords: wood biodegradation; enzymes; low molecular weight compounds.A biodegradação de materiais lignocelulósicos, como a madeira, é um processo natural de reciclagem de matéria orgânica e o entendimento deste processo contribui com a compreensão do ciclo natural do carbono, que tem início na biossíntese dos seus constituintes a partir de H 2 O, CO 2 e energia solar e termina com a mineralização destes constituintes e liberação de CO 2 e H 2 O, em decorrência da decomposição causada principalmente por fungos.Por muito tempo o processo de biodegradação foi foco de muitos estudos devido à grande quantidade de madeira e produtos à base de madeira, que são danificados por ação de fungos, e devido à necessidade do desenvolvimento de métodos de prevenção à biodegradação menos ofensivos ao meio ambiente. Atualmente, os fungos degradadores de madeira e/ou seus sistemas degradativos têm sido intensivamente estudados em razão do elevado potencial de utilização em aplicações biotecnológicas, como na biodegradação de compostos xenobióticos, 1-3 biorremediação de solos, 1,4,5 biopolpação da madeira 6,7 e biobranqueamento de polpas celulósicas. [8][9][10] Uma vez que o desenvolvimento e a eficácia destes métodos/processos dependem principalmente do conhecimento dos mecanismos químicos e bioquímicos envolvidos na biodegradação dos constituintes da madeira, o entendimento destes mecanismos certamente irá contribuir para o desenvolvimento de novas aplicações, assim como para a melhoria das já existentes.Apesar do considerável conhecimento adquirido nas últimas dé-cadas a respeito da enzimologia da biodegradação dos constituintes da madeira, até os dias de hoje não se conhecem os mecanismos químicos e bioquímicos exatos empregados pelos fungos durante este processo. Contudo, sabe-se que o processo de biodegradação da madeira por fungos não pode ser explicado unicamente com base na ação das diversas enzimas lignocelulolíticas por eles secretadas. Dentro dessa abordagem, pesquisas recentes têm indicado uma participação expressiva de compostos de baixa massa molar (CBMM). 11,12Neste sentido, esta revisão tem como objetivo principal agrupar as diferentes teorias envolvendo CBMM produ...

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Section: Peptídeosunclassified

Relevância de compostos de baixa massa molar produzidos por fungos e envolvidos na biodegradação da madeira

Arantes¹,

Milagres²

2009

Quím. Nova

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“…OH produced in wooddegrading cultures of P. chrysosporium, and we have proposed that . OH plays a role in lignin degradation in combination with phenol oxidases Tanaka et al, 1996; 1999a . An extracellular substance from Irpex lacteus, which was similar to the P. chrysosporium glycoproteins, hydroxylated phenol to catechol and hydroquinone Tanaka et al, 1991 .…”

Section: Organismsmentioning

confidence: 99%

“…OH can cause aromatic demethoxylation and hydroxylation, -O-4 bond cleavage, and C -oxidation in lignin from wood degraded by the brown-rot fungus Gloeophyllum trabeum Filley et al, 2002;Hammel et al, 2002 . Arantes and Milagres 2007 reported synergistic activity between ligninolytic enzymes, such as MnP and have 2,3-enediols with reducing activity. The 2,3-enediol may coordinate Fe III and reduce it to Fe II Sakurai et al, 1990;Tanaka et al, 1996 , since these compounds are known to reduce O 2 to H 2 O 2 and Fe III to Fe II Oak et al, 2000 . Thus, Fe-containing glycosylated proteins could repeatedly reduce O 2 to . OH via H 2 O 2 in the presence of certain electron donors, as previously described Enoki et al, 2003 .…”

Section: Introductionmentioning

confidence: 99%

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Presence of Extracellular NAD<sup>+</sup> and NADH in Cultures of Wood-Degrading Fungi

et al. 2015

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Our previous studies indicated that extracellular glycoproteins produced by some white-rot and brown-rot basidiomycetous fungi reduce Fe(III) to Fe(II) and O 2 to H 2 O 2 and produce hydroxyl radicals. The continuous generation of hydroxyl radicals requires a constant supply of O 2 and an electron donor for the reduction of oxidized forms of the glycoproteins to the reduced forms. However, electron donors for this reaction, such as NADH, have not been identified. In this study, the amounts of the extracellular pyridine coenzymes, NAD and NADH, were measured in agar cultures of four white-rot fungi, one brown-rot fungus, and three soft-rot fungi. The sums of NAD and NADH detected in wood-containing cultures of all five basidiomycetes were greater than those in glucose cultures. The amounts of NAD were higher than those of NADH in all wood-containing cultures except that of Irpex lacteus, and NAD was greater than NADH in all glucose cultures except that of Fomitopsis palustris. Significant amounts of pyridine coenzymes were present in glucose and wood-containing cultures of the three soft-rot fungi. The non-wood-degrading fungus, Penicillium funiculosum, did not produce NAD or NADH in either glucose or wood-containing cultures. The extracellular pyridine coenzyme levels were relatively high compared to the rates of extracellular hydroxyl radical generation in wood-degrading fungal cultures. Thus, white-, brown-, and soft-rot fungi produce pyridine coenzymes that could serve as electron donors for the production of hydroxyl radicals during wood degradation.Key words White-rot fungi / Brown-rot fungi / Soft-rot fungi / Hydroxyl radical / NAD and NADH.

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“…Iron binding compounds have been also reported to actively participate in the decomposition of wood by several fungi (Goodell et al 1997;Machuca et al 1999;Milagres et al 2002;Tanaka et al 1993;Tanaka et al 1996).…”

Section: Introductionmentioning

confidence: 99%

Accumulation of iron-binding compounds in root of Pinus sylvestris challenged by Heterobasidion annosum sensu lato

et al. 2015

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Abstract:We examined the interaction between the roots of Pinus sylvestris and closely related species Heterobasidion annousum s.l. (H. annosum s.s., H. parviporum, H. abietinum) that differ in host plant preference. The aim of the current study was to determine in roots the accumulation pattern of low molecular mass compounds such as catecholate and hydroxamate derivates, oxalic acid as well as iron-reduction ability of that low molecular mass compounds, that play important roles in wood degradation and they are also involved in pathogenesis. The accumulation of catechol and hydroxamate derivates increased during the early (2-6 h) and late (24-48 h) stages of interaction and similar pattern of oxalic acid accumulation were observed. The level of catecholate derivates in P. sylvestris roots that were challenged with H. parviporum or H. abietinum correlated strongly with iron reducing ability. However, when host was exposed to H. annosum s. s. hydroxamates rather than catecholates regulated iron reducing ability. The extracellular Fe 3+ reducing activity was greater for H. annosum s. s. isolates than for isolates of two other species, and reduction of ferric iron may promote oxidative burst in host cell and fungal colonization. Catecholate concentration in the presence of H. annosum s.s. contributing to host cell death, confirm iron involvement in infection success.

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An Extracellular Substance from the White-Rot Basidiomycete Phanerochaete chrysosporium for Reducing Molecular Oxygen and Ferric Iron

Cited by 28 publications

References 38 publications

Relevância de compostos de baixa massa molar produzidos por fungos e envolvidos na biodegradação da madeira

Relevância de compostos de baixa massa molar produzidos por fungos e envolvidos na biodegradação da madeira

Presence of Extracellular NAD<sup>+</sup> and NADH in Cultures of Wood-Degrading Fungi

Accumulation of iron-binding compounds in root of Pinus sylvestris challenged by Heterobasidion annosum sensu lato

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