An HBT-based fluorescent probe for nitroreductase determination and its application in <i>Escherichia coli</i> cell imaging

Yang, Quanzhi; Wen, Yuqing; Zhong, Aiguo; Xu, Jian; Shao, Shijun

doi:10.1039/d0nj03286g

Cited by 48 publications

(8 citation statements)

References 41 publications

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“…[17][18][19][20] Taking our inspiration from recent fluorescence-based NTR sensors, functionalization of a hydroxyl with p-nitroanisole results in an effective NTR responsive unit. 21 Here, we show that TCF-Nitro can be used to sensitively and selectively detect NTR and monitor NTR levels in E. coli. as well as a range of other bacterial species and strains.…”

mentioning

confidence: 74%

“…Taking our inspiration from recent fluorescence-based NTR sensors, functionalization of a hydroxyl with p -nitroanisole results in an effective NTR responsive unit. 21 1,8-naphthalimide and dicyanomethylene 4 H -pyran (DCM) have been used as the cores of NTR-responsive systems by Zhang et al and Yang et al , 22,23 demonstrating the effective sensing of NTR in solution and in Hep-G2 cells. Although NTR expression among E. coli has been verified, bacterial-based sensing of NTR remains unknown.…”

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confidence: 99%

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A TCF-based fluorescent probe to determine nitroreductase (NTR) activity for a broad-spectrum of bacterial species

et al. 2023

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confidence: 74%

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confidence: 99%

A TCF-based fluorescent probe to determine nitroreductase (NTR) activity for a broad-spectrum of bacterial species

et al. 2023

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“…Nitroreductase (NTR) is a biomarker that was overexpressed in the hypoxic tumor microenvironment. ,− Generally, under hypoxic microenvironment, NTR can undergo an enzyme-induced reduction from a nitro group to the corresponding amine group in the presence of NADH as an electron donor, which is a common strategy for the design of hypoxia PA probes. , On the basis of this, Wu’s group developed an activatable and dual-mode probe (Q-NO 2 ) responsive toward NTR for visualization of breast cancer metastasis through PA and fluorescence imaging (Figure a) . Q-NO 2 is composed of a NIR chromophore and a NTR-recognition group (nitrobenzol).…”

Section: Activatable Pa Probes For Molecular Detectionmentioning

confidence: 99%

Chemical Design of Activatable Photoacoustic Probes for Precise Biomedical Applications

et al. 2022

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Photoacoustic (PA) imaging technology, a three-dimensional hybrid imaging modality that integrates the advantage of optical and acoustic imaging, has great application prospects in molecular imaging due to its high imaging depth and resolution. To endow PA imaging with the ability for real-time molecular visualization and precise biomedical diagnosis, numerous activatable molecular PA probes which can specifically alter their PA intensities upon reacting with the targets or biological events of interest have been developed. This review highlights the recent developments of activatable PA probes for precise biomedical applications including molecular detection of the biotargets and imaging of the biological events. First, the generation mechanism of PA signals will be given, followed by a brief introduction to contrast agents used for PA probe design. Then we will particularly summarize the general design principles for the alteration of PA signals and activatable strategies for developing precise PA probes. Furthermore, we will give a detailed discussion of activatable PA probes in molecular detection and biomedical imaging applications in living systems. At last, the current challenges and outlooks of future PA probes will be discussed. We hope that this review will stimulate new ideas to explore the potentials of activatable PA probes for precise biomedical applications in the future.

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“…Yang and coworkers also reported the development of probe 6 for tracing NTR in E. coli [46] . Probe 6 on titration with NTR and NADH release 6 a , which emits fluorescence at 633 nm (Figure 6).…”

Section: Small Molecule Based Ntr Probesmentioning

confidence: 99%

Small Molecule Fluorescent Probes for Sensing and Bioimaging of Nitroreductase

et al. 2022

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Hypoxia is a condition caused by a decrease in oxygen and is often seen in cases of cardiac ischemia, solid tumors, inflammation, and other diseases. Tumor hypoxia can result in cancer invasion, metastasis, decreased apoptosis, and resistance to various therapies (chemotherapy, immunotherapy, and radiotherapy), ultimately reducing overall survival rates. These effects of hypoxia underline the need to monitor hypoxia‐associated specific markers for more effective therapeutic intervention. Nitroreductase (NTR) is a common biomarker of hypoxia, via its reduction activity with nitroaromatic compounds to corresponding amino derivatives using NADH and NADPH as cofactor. The NTR expression level can be correlated to cellular hypoxia. Therefore, developing a diagnostic tool for measuring NTR activity is of immense importance for improving treatment efficacies. To leverage NTR activity as a proxy of hypoxia, small molecule based fluorescent probes have been developed as quantifiable probes. To date, several NTR‐sensitive fluorescent probes have been reported. This review is focuses on the advances in these fluorescent NTR probes over the last five years.

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An HBT-based fluorescent probe for nitroreductase determination and its application in Escherichia coli cell imaging

Abstract: A novel HBT-based fluorescent probe HBTPN was reasonably designed for detecting nitroreductase by merging 4-nitrobenzyl as a sensing moiety to HBT-based dye HBTPP. Experiments showed that probe HBTPN displayed selective...

Cited by 48 publications

References 41 publications

A TCF-based fluorescent probe to determine nitroreductase (NTR) activity for a broad-spectrum of bacterial species

A TCF-based fluorescent probe to determine nitroreductase (NTR) activity for a broad-spectrum of bacterial species

Chemical Design of Activatable Photoacoustic Probes for Precise Biomedical Applications

Small Molecule Fluorescent Probes for Sensing and Bioimaging of Nitroreductase

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