An immunochemical and immunohistochemical study of S100 protein in renal cell carcinoma

Abstract

The authors localized S100 protein in renal tubules and renal cell carcinoma by immunohistochemical study and quantitative analysis by enzyme immunoassay. The alpha subunit of S100 protein (S100-alpha) was localized in epithelial cells of proximal tubules, thin limbs of loops of Henle, collecting tubules, and a few of Bowman's capsules. The beta subunit (S100-beta) was immunostained in the distal tubules, thick and thin loops of Henle, collecting tubules, and a few proximal tubules. In renal cell carcinoma, S100-alpha was immunohistochemically demonstrated in 82% (41/50) of patients including sarcomatoid variants, whereas S100-beta was detected in 46% (23/50). Both the number of positively stained tumor cells and the staining intensity were greater in S100-alpha than in S100-beta. Concentrations of S100-alpha in the cortex were 80.3 +/- 22.5 ng/mg protein (n = 7), whereas those of renal cell carcinoma were 387 +/- 533 ng/mg protein (n = 19), i.e., about five times higher. Concentrations of S100-beta in both normal kidney (1.96 +/- 0.74 ng/mg protein) and renal cell carcinoma (2.05 +/- 2.16 ng/mg protein) were much lower than those of S100-alpha. The authors also localized S100-alpha and S100-beta in tissues of other renal tumors and tumors arising from other organs. S100a0 appears to be a useful immunohistochemical marker for renal cell carcinoma.

Oncocytic carcinoma of parotid gland: a case report with clinical, immunohistochemical and ultrastructural features

“…This variant of renal carcinoma is characteristically composed of cells organized in sheets, cords or as papillary fronds [ 9 ]. On immunohistochemical examination, oncocytic carcinoma in a salivary gland is negative for carcinoembryonal antigen (CEA) and S-100 protein [ 10 ] in contrast with renal carcinoma [ 11 , 12 ] which is positive to either markers. In our case, the diagnosis of primary oncocytic carcinoma of parotid gland was made by immunohistochemical analysis revealing negativity for S100 and CEA according to other studies reported in the literature [ 10 ] and by a negative clinical history for a renal tumour.…”

Monocyte/Macrophage-directed antibodies Ki-M3 and Ki-M7 detect renal-cell carcinomas

“…Positivity for renal-cell carcinomas was also demonstrated with CALLA and OKB2 (CD24) MAbs (Bohle et al, 1986). Recently, renal-cell Carcinomas were shown to be positive with an antibody against S-100 protein (Takahashi et al, 1988). However, all of these antibodies, listed in Table 111, are more or less specific for renal-cell carcinomas.…”

Friend or Foe: S100 Proteins in Cancer

“…In the 1980s, soon after the identification of the first S100 family members, the presence of S100 proteins was also observed in different cancer types, drawing rising attention to this group of calcium-binding proteins within the oncological field. In this context, S100 expression was, for example, detected in metastatic melanoma [70,71], renal cell carcinoma [72], and breast cancer [73]. Moreover, an S100 protein expressed in mouse Ehrlich ascites-tumour cells was identified as S100A6 [74], and S100P was found in the cell nuclei of brain tumours [75].…”