An immunocytochemical method for studying patterns of cell proliferation in the wool follicle

Holle, S. A.; Birtles, M. J.

doi:10.1080/00480169.1990.35625

Cited by 19 publications

(15 citation statements)

References 21 publications

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“…Incorporation rate of BrdU into the internal organ tissue (an index of cell mitotic activity) was deter mined by an immunocytochemical technique [28,29], Cells labelled with BrdU were detected by an immunostain with a monocolonal antibody against BrdU (Amersham, England). Pancreatic endocrine cells were identified with a standard immunocytochemical tech nique [30].…”

Section: Histological and Morphological Analysesmentioning

confidence: 99%

Effects of Oral IGF-I or IGF-II on Digestive Organ Growth in Newborn Piglets

Mellor

Birtles

et al. 1994

Neonatology

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To study whether colostrum-borne growth factors are responsible for the rapid GI tissue growth in naturally suckled newborn animals, newborn unsuckled piglets were bottle-fed for 24 h with infant milk formula with or without addition of 2 μg/ml of recombinant human insulin-like growth factor-I (IGF-I) or insulin-like growth factor-II (IGF-II), a level which approximated that of porcine colostrum. The animals were then sacrificed for measurements of their digestive organ weights and contents of protein, RNA and DNA in the organs. The treatment with IGF-I or IGF-II failed to show any significant effect on the weight of the esophagus, stomach, small intestine, large intestine, mandibular glands, kidneys and the spleen, and had no effects on the contents of protein, RNA and DNA in the small intestinal mucosa, the liver and the spleen. However, piglets fed with infant formula containing IGF-I (n = 7) or IGF-II (n = 7) had a heavier pancreas (p < 0.05) compared to formula-fed controls (n = 7). The DNA content in the stomach and the pancreas were greater in animals treated with IGF-I or IGF-II than in controls. Using a cell labelling technique it was shown that both IGF-I and IGF-II stimulated cell proliferation in the small intestinal crypts. The results indicate that the substantial GI tissue growth reported in newborn animals is unlikely due to colostrum-borne IGF-I or IGF-II alone. On the other hand the study does suggest that oral IGF-I and IGF-II are capable of stimulating cell proliferation in the GI tract.

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Section: Histological and Morphological Analysesmentioning

confidence: 99%

Effects of Oral IGF-I or IGF-II on Digestive Organ Growth in Newborn Piglets

Mellor

Birtles

et al. 1994

Neonatology

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“…Although BrdU pulse labelling was previously used in sheep to assess cell proliferation in wool follicles [15], to our knowledge, there has been no prior report using this technique to identify proliferating cells in the airway wall. The time-dependent changes in BrdU-expression in this model proved to be qualitatively consistent with our previous findings where Ki-67 positivity was used as our proliferative marker [13].…”

Section: Discussionmentioning

confidence: 99%

BrdU Pulse Labelling In Vivo to Characterise Cell Proliferation during Regeneration and Repair following Injury to the Airway Wall in Sheep

Yahaya

McLachlan

Collie

2013

The Scientific World Journal

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The response of S-phase cells labelled with bromodeoxyuridine (BrdU) in sheep airways undergoing repair in response to endobronchial brush biopsy was investigated in this study. Separate sites within the airway tree of anaesthetised sheep were biopsied at intervals prior to pulse labelling with BrdU, which was administered one hour prior to euthanasia. Both brushed and spatially disparate unbrushed (control) sites were carefully mapped, dissected, and processed to facilitate histological analysis of BrdU labelling. Our study indicated that the number and location of BrdU-labelled cells varied according to the age of the repairing injury. There was little evidence of cell proliferation in either control airway tissues or airway tissues examined six hours after injury. However, by days 1 and 3, BrdU-labelled cells were increased in number in the airway wall, both at the damaged site and in the regions flanking either side of the injury. Thereafter, cell proliferative activity largely declined by day 7 after injury, when consistent evidence of remodelling in the airway wall could be appreciated. This study successfully demonstrated the effectiveness of in vivo pulse labelling in tracking cell proliferation during repair which has a potential value in exploring the therapeutic utility of stem cell approaches in relevant lung disease models.

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“…Van Leeuwen et al (1995) also noted significant variability in the gut morphology of swine. Preliminary work from this laboratory using 5-bromo-2'-deoxyuridine (BrdU) to measure cell proliferation (Holle and Birtles 1990), found that cell turnover in the small intestine was reduced (P < 0.0001) when L. pedunculatus (66.6 ± 8.9 mitotic cells mm -2 h -1 ) was fed, compared to sheep consuming pasture (174.8 ± 8.0 mitotic cells mm -2 h -1 ) (Walton, Waghorn, Plaizier, Birtles and McBride, unpublished data). The impact of reduced cell turnover on nutrient digestibility in the small intestine of sheep remains unknown and requires further investigation.…”

Section: Mots Clésmentioning

confidence: 99%

“…Gut contents were removed, and the gut was flushed with ice cold saline and samples were collected from the proximal and distal duodenum, proximal, mid-and distal jejunum and ileum, and ventral rumen. All tissues were excised within 12 min of death and fixed immediately in Bouins fixative for about 5 h, then transferred to graded ethanol solutions, starting with 70% vol/vol up to 100% (Holle and Birtles 1990). Histological sections were stained with haematoxylin and eosin (HE staining), embedded in paraffin wax and sectioned as described by Holle and Birtles (1990).…”

Section: Mots Clésmentioning

confidence: 99%

“…All tissues were excised within 12 min of death and fixed immediately in Bouins fixative for about 5 h, then transferred to graded ethanol solutions, starting with 70% vol/vol up to 100% (Holle and Birtles 1990). Histological sections were stained with haematoxylin and eosin (HE staining), embedded in paraffin wax and sectioned as described by Holle and Birtles (1990). Morphometric measurements of the proximal and distal duodenum, proximal, mid-, and distal jejunum and ileum, and ventral rumen were determined using computeraided light microscope image analysis (BioQuant System IV, R & M Biometrics, Nashville, TN) according to Bird et al (1994).…”

Section: Mots Clésmentioning

confidence: 99%

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Influence of condensed tannins on gut morphology in sheep fed Lotus pedunculatus

Walton¹,

Waghorn²,

Plaizier³

et al. 2001

Can. J. Anim. Sci.

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can reduce intestinal absorption of amino acids (AA) and peptides. This experiment tested the hypothesis that CT in Lotus pedunculatus altered small intestinal mucosal morphology reducing absorptive capacity of the gut. Thirteen young Romney wethers were fed ad libitum either Lotus pedunculatus (cv. Grasslands Maku, N = 7) containing 5.5% CT in the dry matter (DM) or white cloverperennial ryegrass pasture (N = 6), which did not contain CT, for 4 wk. The wethers were euthanized at the end of the feeding trial and samples were collected for histological examination from the proximal and distal duodenum, proximal, mid-, and distal jejunum and ileum, and ventral rumen. There was no effect of feeding L. pedunculatus containing 5.5% CT (P > 0.05) on rumen or gut morphological parameters in sheep. The cause of reduced AA absorption in the presence of CT is not known, but this study suggests it is not due to changes in intestinal morphology. L'expérience devait établir si les tannins concentrés qu'on trouve dans Lotus pedunculatus entraînent une modification morphologique de la muqueuse de l'intestin grêle et diminuent la capacité d'absorption de cet organe. Pendant quatre semaines, 13 jeunes béliers castrés Romney ont reçu à satiété du fourrage de L. pedunculatus (cv. Grasslands Maku, N = 7) contenant 5,5 % de tannins dans la matière sèche, ou un mélange de trèfle blanc et d'élyme vivace (N = 6) sans tannin. Les animaux ont ensuite été sacrifiés et on a prélevé des échantillons des parties proximales et distale du duodénum, des parties proximale, intermédiaire et distale du jéjunum et de l'iléon, et du rumen ventral pour les soumettre à un examen histologique. L'absorption de Lotus pedunculatus renfermant 5,5% de tannins n'a aucune incidence (P > 0,05) sur les paramètres morphologiques du rumen ou de l'intestin chez les ovins. On ignore pourquoi les tannins, en grande quantité, réduisent l'absorption des acides aminés, mais les résultats de la présente étude laissent croire qu'une modification de la morphologie de l'intestin n'est pas en cause. Mots clés: Lotus pedunculatus, tannins concentrés, morphologie de l'intestin, ovinsCondensed tannins are polyphenolic compounds commonly found in the seed coats of many plants (e.g., sorghum, alfalfa), and in the foliage of several legumes and grass cultivars (Waghorn 1996). Condensed tannins have been shown to protect plant proteins from degradation in the rumen of sheep. However, the apparent absorption of AA from the small intestine of sheep appears to be impaired by the CT in L. pedunculatus (Waghorn et al. 1994). Condensed tannins fed at low to medium concentrations in DM (up to 4% DM) usually result in increased N retention or AA flow to the intestine, but decreased in N digestibility over the entire digestive tract. The increased N retention is due to a greater supply of protein, and perhaps an improved ratio of nonessential:essential AA available for absorption (Waghorn et al. 1987).Condensed tannins bind to proteins in a pH range of about 5.0-8.0 and dissociate at a pH ...

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An immunocytochemical method for studying patterns of cell proliferation in the wool follicle

Cited by 19 publications

References 21 publications

Effects of Oral IGF-I or IGF-II on Digestive Organ Growth in Newborn Piglets

Effects of Oral IGF-I or IGF-II on Digestive Organ Growth in Newborn Piglets

BrdU Pulse Labelling In Vivo to Characterise Cell Proliferation during Regeneration and Repair following Injury to the Airway Wall in Sheep

Influence of condensed tannins on gut morphology in sheep fed Lotus pedunculatus

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