An immunohistochemical marker panel including claudin-18, maspin, and p53 improves diagnostic accuracy of bile duct neoplasms in surgical and presurgical biopsy specimens

Diagnostic value of maspin in distinguishing adenocarcinoma from benign biliary epithelium on endoscopic bile duct biopsy

“…A total of 58 endobiliary forceps biopsy specimens were also examined for maspin expression in that study, including 18 adenocarcinomas, 19 nonneoplastic samples and 21 atypical lesions. The results were similar to those of the surgical specimens [22]. In the present study, we extended our…”

“…Of positive cases, 13 (43%) showed a diffuse and strong staining pattern. These findings are similar to those reported for gallbladder carcinomas as described above, and are further supported by a more recent study that also showed combined nuclear/cytoplasmic maspin immunoreactivity in 59 resected intrahepatic and extrahepatic bile duct carcinomas [22]. A total of 58 endobiliary forceps biopsy specimens were also examined for maspin expression in that study, including 18 adenocarcinomas, 19 nonneoplastic samples and 21 atypical lesions.…”

“…Recently, the biological functions of maspin have been linked to its subcellular localization [3,4]. There are only a few publications that have examined maspin expression in biliary cancers [10,[19][20][21][22][23].…”

Immunocytochemistry for Claudin‐18 and Maspin in biliary brushing cytology increases the accuracy of diagnosing pancreatobiliary malignancies

“…In our study, Claudin‐18 was distributed along the entire cell membrane of most adenocarcinoma cells but was almost completely absent from non‐neoplastic epithelial cells. These findings are in agreement with previous histological studies of biopsy or surgical specimens of the bile duct or pancreas …”

Role of CD15 expression in dysplastic and neoplastic tissue of the bile duct – a potential novel tool for differential diagnosis of indeterminate biliary stricture

“…On the other hand, CD15 might be a suitable instrument for the differential diagnosis of IBS from intraductal biopsies and brush cytologies. Sensitivity in our study is comparable to immunohistochemical studies demonstrating that combined immunohistochemical staining of three different antibodies can differentiate between malignant and non‐malignant bile duct biopsies in 67–86% . This is higher than sensitivity of fluorescence in‐situ hybridization (FISH), which was reported to be 42.9% in a study from 2009 with 498 biliary brush samples .…”