An Immunohistochemistry-Based Study on Aquaporin (AQP)-1, 3, 4, 5 and 8 in the Parotid Glands, Submandibular Glands and Sublingual Glands of Sj^|^ouml;gren^|^rsquo;s Syndrome Mouse Models Chronically Administered Cevimeline

Nakamura, Moriyoshi; Saga, Tsuneo; Watanabe, Koichi; Takahashi, Nagahiro; Tabira, Yoko; Kusukawa, Jingo; Yamaki, Koh-Ichi

doi:10.2739/kurumemedj.ms60227

Cited by 16 publications

(21 citation statements)

References 17 publications

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“…In our study, AQP5 was expressed on the membranes of MEC mucous cells. In normal salivary glands, AQP5 was identified in acinar structures, as previously described [24,25,27,48]. In vitro evaluation also demonstrated the presence of AQP5 in MEC cells.…”

Section: Discussionsupporting

confidence: 65%

Aquaporin 1, 3, and 5 Patterns in Salivary Gland Mucoepidermoid Carcinoma: Expression in Surgical Specimens and an In Vitro Pilot Study

Stamboni

Gomes

Souza

et al. 2020

IJMS

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Salivary gland aquaporins (AQPs) are essential for the control of saliva production and maintenance of glandular structure. However, little is known of their role in salivary gland neoplasia. Salivary gland tumors comprise a heterogeneous group of lesions, featuring variable histological characteristics and diverse clinical behaviors. Mucoepidermoid carcinoma (MEC) is the most common salivary gland malignancy. The aim of this study was to evaluate the expression of AQP1, AQP3, and AQP5 in 24 MEC samples by immunohistochemistry. AQP1 expression was observed in vascular endothelium throughout the tumor stroma. AQP3 was expressed in epidermoid and mucosal cells and AQP5 was expressed in mucosal cells of MEC. These proteins were expressed in the human MEC cell line UH-HMC-3A. Cellular ultrastructural aspects were analyzed by electron microscopy to certificate the tumor cell phenotype. In summary, our results show that, despite the fact that these molecules are important for salivary gland physiology, they may not play a distinct role in tumorigenesis in MEC. Additionally, the in vitro model may offer new possibilities to further investigate mechanisms of these molecules in tumor biology and their real significance in prognosis and possible target therapies.

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Section: Discussionsupporting

confidence: 65%

Aquaporin 1, 3, and 5 Patterns in Salivary Gland Mucoepidermoid Carcinoma: Expression in Surgical Specimens and an In Vitro Pilot Study

Stamboni

Gomes

Souza

et al. 2020

IJMS

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“…AQP3, AQP4 and AQP8 are located at the basal region of acinar cells and in ductal cells [ 13 , 21 , 23 ]. AQP8 is also expressed by myoepithelial cells [ 24 ]. Both the apical and basolateral membranes of acinar cells display positive AQP5 labeling [ 17 , 22 , 25 , 26 ].…”

Section: Cellular Distribution Of Aqps In Adult Sg Subsectionmentioning

confidence: 99%

Insight into Salivary Gland Aquaporins

D’Agostino

Elkashty

Chivasso

et al. 2020

Cells

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The main role of salivary glands (SG) is the production and secretion of saliva, in which aquaporins (AQPs) play a key role by ensuring water flow. The AQPs are transmembrane channel proteins permeable to water to allow water transport across cell membranes according to osmotic gradient. This review gives an insight into SG AQPs. Indeed, it gives a summary of the expression and localization of AQPs in adult human, rat and mouse SG, as well as of their physiological role in SG function. Furthermore, the review provides a comprehensive view of the involvement of AQPs in pathological conditions affecting SG, including Sjögren’s syndrome, diabetes, agedness, head and neck cancer radiotherapy and SG cancer. These conditions are characterized by salivary hypofunction resulting in xerostomia. A specific focus is given on current and future therapeutic strategies aiming at AQPs to treat xerostomia. A deeper understanding of the AQPs involvement in molecular mechanisms of saliva secretion and diseases offered new avenues for therapeutic approaches, including drugs, gene therapy and tissue engineering. As such, AQP5 represents a potential therapeutic target in different strategies for the treatment of xerostomia.

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“…Three-time daily intake of 30 mg of cevimeline or of three- to four-time daily intake of pilocarpine represent therapeutic options, with limited side effects, for the treatment of xerostomia in SS patients and head and neck irradiated patients [ 80 ]. At a molecular level, cevimeline was shown to prevent radiation-induced xerostomia and radiation-induced decrease in AQP5 expression in mice submandibular glands [ 83 ], and normalized AQP5 localization to the apical membrane of SG acinar cells in SS mice [ 84 , 85 ]. The effects of bethanechol, a carbamate ester resistant to cholinesterase, acting on M3 muscarinic receptors has been tested in patients with radiotherapy-induced xerostomia, but additional studies are required to assess the beneficial effects of the drug in the treatment of saliva hypofunction of other origins, including pSS [ 86 ].…”

Section: Role Of Aqps In the Treatment Of Sjögren’s Syndromementioning

confidence: 99%

Involvement of Aquaporins in the Pathogenesis, Diagnosis and Treatment of Sjögren’s Syndrome

Soyfoo

Chivasso

Perret

et al. 2018

IJMS

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Sjögren’s syndrome (SS) is a chronic autoimmune disease characterized by lymphocytic infiltration of salivary and lacrimal glands resulting in diminished production of saliva and tears. The pathophysiology of SS has not yet been fully deciphered. Classically it has been postulated that sicca symptoms in SS patients are a double step process whereby lymphocytic infiltration of lacrimal and salivary glands (SG) is followed by epithelial cell destruction resulting in keratoconjunctivitis sicca and xerostomia. Recent advances in the field of the pathophysiology of SS have brought in new players, such as aquaporins (AQPs) and anti AQPs autoantibodies that could explain underlying mechanistic processes and unveil new pathophysiological pathways offering a deeper understanding of the disease. In this review, we delineate the link between the AQP and SS, focusing on salivary glands, and discuss the role of AQPs in the treatment of SS-induced xerostomia.

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An Immunohistochemistry-Based Study on Aquaporin (AQP)-1, 3, 4, 5 and 8 in the Parotid Glands, Submandibular Glands and Sublingual Glands of Sj^|^ouml;gren^|^rsquo;s Syndrome Mouse Models Chronically Administered Cevimeline

Cited by 16 publications

References 17 publications

Aquaporin 1, 3, and 5 Patterns in Salivary Gland Mucoepidermoid Carcinoma: Expression in Surgical Specimens and an In Vitro Pilot Study

Aquaporin 1, 3, and 5 Patterns in Salivary Gland Mucoepidermoid Carcinoma: Expression in Surgical Specimens and an In Vitro Pilot Study

Insight into Salivary Gland Aquaporins

Involvement of Aquaporins in the Pathogenesis, Diagnosis and Treatment of Sjögren’s Syndrome

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