An immunomagnetic separation/loop-mediated isothermal amplification method for rapid direct detection of thermotolerant Campylobacter spp. during poultry production

Abstract

The method offers an inexpensive approach to monitor poultry infection in near real time, assisting flock management and controls to prevent introduction of Campylobacter into the food chain.

A loop-mediated isothermal amplification method for rapid direct detection and differentiation of nonpathogenic and verocytotoxigenicEscherichia coliin beef and bovine faeces

“…Romero et al . () developed a rapid LAMP assay that was able to detect thermotolerant Campylobacter sp. in boot swab samples, without the use of culture enrichment or DNA extraction.…”

“…A LAMP assay detection limit of 4Á1 9 10 4 CFU per ml for detection of E. coli O157 in raw milk, without the incorporation of an enrichment step in the procedure, has previously been reported (Wang et al 2009). Romero et al (2015) developed a rapid LAMP assay that was able to detect thermotolerant Campylobacter sp. in boot swab samples, without the use of culture enrichment or DNA extraction.…”

A Rapid LAMP-Based Method for Screening Poultry Samples for Campylobacter Without Enrichment

“…The LAMP assay was described previously ( Romero et al, 2016 ). It targets a region of the thermotolerant Campylobacter spp.…”

“…We previously developed a LAMP assay for the detection of themotolerant Campylobacter spp. ( Romero et al, 2016 ) in poultry boot swabs. The procedure involved the use of immunomagnetic beads to isolate the bacteria from boot swab samples followed by detection via LAMP.…”

Potential Use of DNA Aptamer-Magnetic Bead Separation-PCR Assay for Salmonella Detection in Food

“…To increase the sensitivity, specificity, and rapidity of detection method, the pre-analytical sample preparation step is important to separate, concentrate, and purify the target bacteria from the food samples (Brehm-stecher et al, 2009;Stevens and Jaykus, 2004). Immuno Magnetic Separation (IMS) that involves the use of specific antibody for coating the surface of magnetic beads has been widely used for pre-analytical sample preparation of complex food matrix (Jenïkovâ et al, 2000;Romero et al, 2016;Wang et al, 2013Wang et al, , 2014Xiong et al, 2014). However, antibody is relatively unstable, with short shelf-life, expensive, and difficult to produce (Famulok and Mayer, 2011;Jayasena, 1999).…”