An impedimetric biosensor for real-time monitoring of bacterial growth in a microbial fermentor

Kim, Young Hun; Park, Jae Sung; Jung, Hyo Il

doi:10.1016/j.snb.2009.01.034

Cited by 52 publications

(21 citation statements)

References 29 publications

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“…SAMs and lipid bilayers are often used as platforms for the immobilization of biosensors [532][533][534], but other supports, such as SiÀSiO 2 [535][536][537], TiO 2 [538], and polymers [539,540], can also be used. Electrochemical biosensors [541,542] might use potentiometric, field-effect transistor, amperometric, or impedimetric transducers.…”

Section: Biosensorsmentioning

confidence: 99%

Electrochemical Impedance Spectroscopy and its Applications

Lasia¹

2014

686

504

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Section: Biosensorsmentioning

confidence: 99%

Electrochemical Impedance Spectroscopy and its Applications

Lasia¹

2014

686

504

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“…Beside direct measurements in the broth, a modified electrode system in an interdigitated design can be used [28,29,30]. First approaches towards process monitoring were shown by Kim et al [31], who worked with an inline sensor used in the lower frequency range between 40 Hz and 10 kHz for real-time monitoring of biomass. Kim et al showed the feasibility for measuring changes in the double layer capacitance, but no analysis of the double layer capacitance (C dl ) itself was performed; only discrete extracted values for distinct frequency values were used.…”

Section: Introductionmentioning

confidence: 99%

A Novel Application for Low Frequency Electrochemical Impedance Spectroscopy as an Online Process Monitoring Tool for Viable Cell Concentrations

Slouka

Wurm

Brunauer

et al. 2016

Sensors

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New approaches in process monitoring during industrial fermentations are not only limited to classical pH, dO2 and offgas analysis, but use different in situ and online sensors based on different physical principles to determine biomass, product quality, lysis and far more. One of the very important approaches is the in situ accessibility of viable cell concentration (VCC). This knowledge provides increased efficiency in monitoring and controlling strategies during cultivations. Electrochemical impedance spectroscopy—EIS—is used to monitor biomass in a fermentation of E. coli BL21(DE3), producing a recombinant protein using a fed batch-based approach. Increases in the double layer capacitance (Cdl), determined at frequencies below 1 kHz, are proportional to the increase of biomass in the batch and fed batch phase, monitored in offline and online modes for different cultivations. A good correlation of Cdl with cell density is found and in order to get an appropriate verification of this method, different state-of-the-art biomass measurements are performed and compared. Since measurements in this frequency range are largely determined by the double layer region between the electrode and media, rather minor interferences with process parameters (aeration, stirring) are to be expected. It is shown that impedance spectroscopy at low frequencies is a powerful tool for cultivation monitoring.

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“…Impedance spectroscopy in the α-dispersion range is until now applied for detection of bacteria in soil, food and feces-polluted water using interdigitated electrodes [27][28][29][30][31][32][33][34][35]. First approaches towards process monitoring were shown in [36]. The authors showed the feasibility for measuring changes in the double-layer capacitance (C DL ).…”

Section: Introductionmentioning

confidence: 99%

Yeast Propagation Control: Low Frequency Electrochemical Impedance Spectroscopy as an Alternative for Cell Counting Chambers in Brewery Applications

Brunauer

Spadiut

Alfred³

et al. 2020

Chemosensors

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Electrochemical impedance spectroscopy is a powerful tool in life science for cell and pathogen detection, as well as for cell counting. The measurement principles and techniques using impedance spectroscopy are highly diverse. Differences can be found in used frequency range (β or α regime), analyzed quantities, like charge transfer resistance, dielectric permittivity of double layer capacitance and in off- or online usage. In recent contributions, applications of low-frequency impedance spectroscopy in the α regime were tested for determination of cell counts and metabolic burden in Escherichia coli and Saccharomyces cerevisiae. The established easy to use methods showed reasonable potential in the lab scale, especially for S. cerevisiae. However, until now, measurements for cell counts in food science are generally based on Thoma cell counting chambers. These microscopic cell counting methods decelerate an easy and quick prediction of yeast viability, as they are labor intensive and result in a time delayed response signal. In this contribution we tested our developed method using low frequency impedance spectroscopy locally at an industrial brewery propagation site and compared results to classic cell counting procedures.

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An impedimetric biosensor for real-time monitoring of bacterial growth in a microbial fermentor

Cited by 52 publications

References 29 publications

Electrochemical Impedance Spectroscopy and its Applications

Electrochemical Impedance Spectroscopy and its Applications

A Novel Application for Low Frequency Electrochemical Impedance Spectroscopy as an Online Process Monitoring Tool for Viable Cell Concentrations

Yeast Propagation Control: Low Frequency Electrochemical Impedance Spectroscopy as an Alternative for Cell Counting Chambers in Brewery Applications

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