An improved acid phosphatase procedure

Babson, Arthur L.; Phillips, George E.

doi:10.1016/0009-8981(66)90303-2

Cited by 63 publications

(23 citation statements)

References 4 publications

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“…The purified enzyme preparation (50-150 fold of purification) thus obtained was homogenous as shown by polyacrylamide electrophoresis. The acid phosphatase activity in serum was measured by a spectro photometric method of Babson and Phillips, using alphanaphthyl phosphate as the substrate [5]. The blood was drawn from patients (more than 50 patients) with prostate cancer or with Gaucher's disease (12 patients, provided by Dr. W f.inrab, Mount Sinai School of Medicine, New York), and the serum was mixed with disodium citrate (1 mg/ml of serum) to preserve acid phosphatase.…”

Section: Methodsmentioning

confidence: 99%

Isoenzymes of Human Prostate Acid Phosphatase

Chu¹,

Wang²,

Merrin³

et al. 1978

Oncology

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The isoenzymes of human prostatic acid phosphatase have been studied by an isoelectric focusing technique. Purified acid phosphatase from malignant prostates contained eight isoenzymes with pI 4.4–5.3. The sera from patients with prostate cancer were shown to have similar acid phosphatase isoenzyme patterns at pI 4.0–5.5; as the serum enzyme activities increased, the pI of isoenzymes shifted to more acidic pH. These isoenzyme patterns of sera from patients with prostate cancer were different from those of patients with Gaucher’s disease or from acid phosphatase of human erythrocytes, both of which exhibited only one enzyme band around pI 5.0 and 6.0, respectively. Treatment of serum sample of prostate cancer with neuraminidase did not result in a single enzyme band but alter the pI of isoenzymes, which shifted to a higher pH region. The significance of acid phosphatase activities and its isoenzyme patterns in prostate cancer merits further investigation.

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Section: Methodsmentioning

confidence: 99%

Isoenzymes of Human Prostate Acid Phosphatase

Chu¹,

Wang²,

Merrin³

et al. 1978

Oncology

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show abstract

“…Of the hydrolytic enzymes only acid phosphatase is commonly determined by diazo coupling, anaphthyl phosphate serving as substrate (Babson and Phillips, 1966). The technique is simple and rapid, and this substrate is more specific for prostatic acid phosphatase than is phenylphosphate.…”

Section: Diazo Coupling Methodsmentioning

confidence: 99%

Recent Advances in Clinical Enzymology

Rosalki

1970

Ann Clin Biochem

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The past year has seen a wider application of knowledge originating over the last decade rather than startling new developments. For brevity, only serum enzymes will be considered and the points at which progress is being made are indicated by the sectional headings which follow. ENZYME METHODOLOGY Considerable change is taking place in established enzyme methods and new forms of methodology are being developed. Colorimetric kinetic assays

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“…Acid phosphatase and LDH were determined in the synovial fluid and concomitant serum specimens by the methods of Babson and Phillips and DiCesare (4,5). Drs.…”

Section: Special Studiesmentioning

confidence: 99%

Chronic Nondestructive Arthritis Associated with Cutaneous Polyarteritis

Smukler

Schumacher

1977

Arthritis & Rheumatism

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Two patients with arthritis of the knee joints associated with cutaneous polyarteritis have been followed for 20 and 5 years. The arthritis is characterized by mild to moderate pain and stiffness and inflammatory joint effusions with predominantly polymorphonuclear leukocytes. Despite its chronicity, there has been no clinical or radiologic evidence of joint destruction. Necrotizing inflammation was seen in arteries of the deep skin but not in the small vessels observed in the synovial biopsy specimens.Cutaneous polyarteritis is characterized by a variety of cutaneous lesions, a benign course, and necrotizing arteritis (1-3). The skin findings, which are often localized to the lower extremities, most frequently include painful erythematous nodules, ulcerations, and livedo reticularis. Cutaneous polyarteritis, however, is not strictly a cutaneous disorder, because arthralgia, arthritis and peripheral neuropathy, and fever may also occur. The generally benign course may be brief, protracted over many years, or episodic with spontaneous or corticosteroid-induced remissions (3). In many patients the white blood cell count and/or erythrocyte sedimentation rate is increased. Diagnosis is confirmed by an excision biopsy of the skin deep enough to detect necrotizing inflammation of arteries in the deep dermis or subcutaneous tissue. These histopathologic findings are identical with those of the skin lesions of systemic polyarteritis. However the predominant cutaneous findings, usual absence of visceral lesions, and benign course of cutaneous polyarteritis are the basis of its delineation from systemic polyarteritis (1-3)We have observed a chronic nondestructive arthritis of the knees in 2 patients with cutaneous polyarteritis for 20 and 5 years. This report describes the clinical features, light microscopic findings in the skin, and light and electron microscopic findings in synovial biopsy specimens of this unusual arthritis. Possible reasons for the benign nature of this joint disease are presented. CASE REPORTS Patient AThis 68-year-old white man developed painful tender skin lesions over the legs and ankles at the outset of his illness, which has now persisted for 20 years. Hospital evaluation at onset showed that he was emaciated, febrile, and normotensive. There were multiple hemorrhagic, exquisitely tender, and indurated lesions measuring 1.5 to 2 cm in diameter over the anterior surface of the legs, the malleoli, and the dorsurn of the feet. Except for pitting edema which extended to the knees

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An improved acid phosphatase procedure

Cited by 63 publications

References 4 publications

Isoenzymes of Human Prostate Acid Phosphatase

Isoenzymes of Human Prostate Acid Phosphatase

Recent Advances in Clinical Enzymology

Chronic Nondestructive Arthritis Associated with Cutaneous Polyarteritis

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