An improved DNA method to unambiguously detect small hive beetle <scp><i>Aethina tumida</i></scp>, an invasive pest of honeybee colonies

Silacci, Paolo; Biolley, C.; Jud, Corinne; Charrière, Jean-Daniel; Dainat, Benjamin

doi:10.1002/ps.5141

Cited by 10 publications

(12 citation statements)

References 8 publications

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“…Aethina tumida Murray, commonly known as the small hive beetle (SHB), belongs to the Nitidulidae family and is a scavenger native to sub-Saharan Africa (Silacci et al 2018). This beetle is considered a pest of honey bees and other social bees like bumble bees and stingless bees (Amos et al 2018) causing significant damage to brood, pollen, and honey stores (Ellis & Hepburn 2006).…”

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Pathogens Detection in the Small Hive Beetle (Aethina tumida (Coleoptera: Nitidulidae))

et al. 2020

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Aethina tumida Murray is currently a worldwide emergent pest of Apis mellifera L. hives. Although the damaging effect on the colony stores and brood is well known, the possible role of these beetles as a disease carrier is not clear. This is the first report of DNA presence of the trypanosome honeybee parasite Lotmaria passim and Crithidia bombi, and the Apis mellifera filamentous virus (AmFV) in A. tumida. Further studies will be needed to determine if A. tumida is indeed a mechanical or biological vector of these pathogens.

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“…The life cycle of the SHB is closely linked to colonies of honey bees (Silacci et al 2018). Females of SHB lay several eggs inside the brood cells of the nests and in the cracks of the hive (Neumann et al 2013).…”

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Pathogens Detection in the Small Hive Beetle (Aethina tumida (Coleoptera: Nitidulidae))

et al. 2020

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“…These include the discoveries of significantly higher infestation levels in areas of forest cover and high precipitation compared to areas of savannah and low precipitation [183], and its association with the yeast Kodamaea ohmeri throughout its entire lifecycle [184]. Genomic sequencing has revealed increased levels of gene families involved in insecticide detoxification, where the quantity of detoxification homologs are substantially different from honey bees [185], and an improved multiplex polymerase chain reaction (PCR) bioassay targeting the cytochrome oxidase subunit I gene, which improves upon previous internal mismatches among nucleotides [186].…”

Section: Parasites Pathogens and Pestsmentioning

confidence: 99%

Impact of Biotic and Abiotic Stressors on Managed and Feral Bees

Belsky

Joshi

2019

Insects

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Large-scale declines in bee abundance and species richness over the last decade have sounded an alarm, given the crucial pollination services that bees provide. Population dips have specifically been noted for both managed and feral bee species. The simultaneous increased cultivation of bee-dependent agricultural crops has given rise to additional concern. As a result, there has been a surge in scientific research investigating the potential stressors impacting bees. A group of environmental and anthropogenic stressors negatively impacting bees has been isolated. Habitat destruction has diminished the availability of bee floral resources and nest habitats, while massive monoculture plantings have limited bee access to a variety of pollens and nectars. The rapid spread and increased resistance buildup of various bee parasites, pathogens, and pests to current control methods are implicated in deteriorating bee health. Similarly, many pesticides that are widely applied on agricultural crops and within beehives are toxic to bees. The global distribution of honey bee colonies (including queens with attendant bees) and bumble bee colonies from crop to crop for pollination events has been linked with increased pathogen stress and increased competition with native bee species for limited resources. Climatic alterations have disrupted synchronous bee emergence with flower blooming and reduced the availability of diverse floral resources, leading to bee physiological adaptations. Interactions amongst multiple stressors have created colossal maladies hitting bees at one time, and in some cases delivering additive impacts. Initiatives including the development of wild flower plantings and assessment of pesticide toxicity to bees have been undertaken in efforts to ameliorate current bee declines. In this review, recent findings regarding the impact of these stressors on bees and strategies for mitigating them are discussed.

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“…The real-time PCR primers and probes tested were from Silacci et al 7 and Ward et al 8 ( Table 1). The SHB assay from Ward et al 8 was used in conjunction with an invertebrate control assay from Silacci et al 7 Real-time PCR reactions were comprised of 1 μL DNA in 10 μL reactions containing 5 μL iTaq™ Universal probes reaction mix (BioRad, Hercules, CA, USA), 375 nmol L -1 of each primer and 125 nmol L -1 probe for the Aethina tumida assay and 200 nmol L -1 of each primer and 50 nmol L -1 probe for the control assay. Reactions were carried out on a ViiA™ 7 real-time PCR system (ThermoFisher Scientific) using the following cycling conditions: 95°C for 1 min followed by 40 cycles of 95°C for 15 s and 60°C for 60 s. Each run included no template (negative) and Aethina tumida (positive) controls.…”

Section: Real-time Pcr Assaymentioning

confidence: 99%

Rapid identification of the invasive Small hive beetle (Aethina tumida) using LAMP

Ponting

Tomkies

Stainton

2020

Pest Management Science

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BACKGROUND Small hive beetle (SHB), Aethina tumida is an invasive pest of managed honey bees and has invaded Europe from Africa. The main risk point identified for incursions of this pest into the UK is imports of package bees and queens. Surveillance of this pest, carried out by the National Bee Unit, involves monitoring imports of live bees and routine screening of sentinel apiaries around the UK through collection of hive debris for visual inspection of pests. Currently, no molecular methods are used for screening hive debris for this pest in the UK. A new invasion of this pest would likely occur at low levels and might present with partial specimens broken up in the hive or eggs from the beetle, difficult to identify by visual inspection. Therefore, we sought to develop a fast and sensitive method for detecting SHB in hive debris by developing a loop‐mediated isothermal amplification (LAMP) assay. RESULT The LAMP assay was able to detect the SHB in 19 to 25.37 min and did not cross‐react with any non‐target species tested. The assay was sensitive and could detect 12pg of DNA and it was able to detect less than 1 mg of tissue in a 30 g complex matrix of honey bee hive debris. The assay could successfully amplify from crude extracts of partial tissue specimens. CONCLUSION This tool will allow rapid field screening of suspect beetle specimens and laboratory screening of hive debris for a notifiable exotic pest of honey bees. © 2020 Crown copyright. Pest Management Science © 2020 Society of Chemical Industry.

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An improved DNA method to unambiguously detect small hive beetle Aethina tumida, an invasive pest of honeybee colonies

Cited by 10 publications

References 8 publications

Pathogens Detection in the Small Hive Beetle (Aethina tumida (Coleoptera: Nitidulidae))

Pathogens Detection in the Small Hive Beetle (Aethina tumida (Coleoptera: Nitidulidae))

Impact of Biotic and Abiotic Stressors on Managed and Feral Bees

Rapid identification of the invasive Small hive beetle (Aethina tumida) using LAMP

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