An improved fluorimetric HPLC method for quantifying tocopherols in Brassica rapa L. subsp. sylvestris after harvest

Annunziata, Maria Grazia; Attico, Assunta; Woodrow, Pasqualina; Oliva, Maria Alessandra; Fuggi, Amodio; Carillo, Petronia

doi:10.1016/j.jfca.2012.05.006

Cited by 22 publications

(15 citation statements)

References 20 publications

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“…Ascorbic acid (ASCAc), dehydroascorbic acid (DHA), reduced and oxidized glutathione (GSH and GSSG) were extracted as described by Annunziata et al (2012) and Woodrow et al (2012) and determined according to Queval and Noctor (2007). Malondialdehyde was assayed according to Carillo et al (2011).…”

Section: Methodsmentioning

confidence: 99%

Durum Wheat Roots Adapt to Salinity Remodeling the Cellular Content of Nitrogen Metabolites and Sucrose

et al. 2017

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Plants are currently experiencing increasing salinity problems due to irrigation with brackish water. Moreover, in fields, roots can grow in soils which show spatial variation in water content and salt concentration, also because of the type of irrigation. Salinity impairs crop growth and productivity by inhibiting many physiological and metabolic processes, in particular nitrate uptake, translocation, and assimilation. Salinity determines an increase of sap osmolality from about 305 mOsmol kg−1 in control roots to about 530 mOsmol kg−1 in roots under salinity. Root cells adapt to salinity by sequestering sodium in the vacuole, as a cheap osmoticum, and showing a rearrangement of few nitrogen-containing metabolites and sucrose in the cytosol, both for osmotic adjustment and oxidative stress protection, thus providing plant viability even at low nitrate levels. Mainly glycine betaine and sucrose at low nitrate concentration, and glycine betaine, asparagine and proline at high nitrate levels can be assumed responsible for the osmotic adjustment of the cytosol, the assimilation of the excess of ammonium and the scavenging of ROS under salinity. High nitrate plants with half of the root system under salinity accumulate proline and glutamine in both control and salt stressed split roots, revealing that osmotic adjustment is not a regional effect in plants. The expression level and enzymatic activities of asparagine synthetase and Δ1-pyrroline-5-carboxylate synthetase, as well as other enzymatic activities of nitrogen and carbon metabolism, are analyzed.

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Section: Methodsmentioning

confidence: 99%

Durum Wheat Roots Adapt to Salinity Remodeling the Cellular Content of Nitrogen Metabolites and Sucrose

et al. 2017

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“…The amounts of hydrogen peroxide (H 2 O 2 ) were determined according to Baptista et al (). Ascorbic acid (ASCAc), dehydroascorbic acid (DHA), reduced and oxidized glutathione (GSH and GSSG) were extracted as described by Annunziata et al () and evaluated according to Queval and Noctor (). Total proteins, starch and sugars were evaluated according to Carillo et al ().…”

Section: Methodsmentioning

confidence: 99%

“…Tocopherols measurement was performed according to a procedure described by Annunziata et al () with some modifications. A total amount of 100 mg of powdered shoots was suspended in 0.5 ml pure methanol with a weight‐to‐volume ratio of 1:5, vortexed and incubated at 30°C for 30 min.…”

Section: Methodsmentioning

confidence: 99%

Durum wheat seedling responses to simultaneous high light and salinity involve a fine reconfiguration of amino acids and carbohydrate metabolism

Woodrow

Ciarmiello

Annunziata

et al. 2016

Physiologia Plantarum

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Durum wheat plants are extremely sensitive to drought and salinity during seedling and early development stages. Their responses to stresses have been extensively studied to provide new metabolic targets and improving the tolerance to adverse environments. Most of these studies have been performed in growth chambers under low light [300-350 µmol m s photosynthetically active radiation (PAR), LL]. However, in nature plants have to face frequent fluctuations of light intensities that often exceed their photosynthetic capacity (900-2000 µmol m s ). In this study we investigated the physiological and metabolic changes potentially involved in osmotic adjustment and antioxidant defense in durum wheat seedlings under high light (HL) and salinity. The combined application of the two stresses decreased the water potential and stomatal conductance without reducing the photosynthetic efficiency of the plants. Glycine betaine (GB) synthesis was inhibited, proline and glutamate content decreased, while γ-aminobutyric acid (GABA), amides and minor amino acids increased. The expression level and enzymatic activities of Δ1-pyrroline-5-carboxylate synthetase, asparagine synthetase and glutamate decarboxylase, as well as other enzymatic activities of nitrogen and carbon metabolism, were analyzed. Antioxidant enzymes and metabolites were also considered. The results showed that the complex interplay seen in durum wheat plants under salinity at LL was simplified: GB and antioxidants did not play a main role. On the contrary, the fine tuning of few specific primary metabolites (GABA, amides, minor amino acids and hexoses) remodeled metabolism and defense processes, playing a key role in the response to simultaneous stresses.

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“…Total carotenoids (µg g −1 dw) were extracted by homogenization of lyophilized spinach leaf tissues (1.0 g) in methanol according to Annunziata et al [49]. The resulting extracts were centrifuged at 4.800× g for 15 min, and carotenoids content was estimated by measuring the absorbance of the supernatant at 470,652 and 665 nm in a polypropylene microplate by a microplate reader (Synergy HT, BioTEK Instruments, Bad Friedrichshall, Germany) according to Wellburn [50].…”

Section: Carotenoids Analysismentioning

confidence: 99%

Morphological and Physiological Responses Induced by Protein Hydrolysate-Based Biostimulant and Nitrogen Rates in Greenhouse Spinach

et al. 2019

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Plant-derived protein hydrolysates (PHs) are gaining prominence as biostimulants due to their potential to improve yield and nutritional quality even under suboptimal nutrient regimens. In this study, we investigated the effects of foliar application of a legume-derived PH (0 or 4 mL L−1) on greenhouse baby spinach (Spinacia oleracea L.) under four nitrogen (N) fertilization levels (0, 15, 30, or 45 kg ha−1) by evaluating morphological and colorimetric parameters, mineral composition, carbohydrates, proteins, and amino acids. The fresh yield in untreated and biostimulant-treated spinach plants increased in response to an increase in N fertilization from 1 up to 30 kg ha−1, reaching a plateau thereafter indicating the luxury consumption of N at 45 kg ha−1. Increasing N fertilization rate, independently of PH, lead to a significant increase of all amino acids with the exception of alanine, GABA, leucine, lysine, methionine, and ornithine but decreased the polyphenols content. Interestingly, the fresh yield at 0 and 15 kg ha−1 was clearly greater in PH-treated plants compared to untreated plants by 33.3% and 24.9%, respectively. This was associated with the presence in of amino acids and small peptides PH ‘Trainer®’, which act as signaling molecules eliciting auxin- and/or gibberellin-like activities on both leaves and roots and thus inducing a “nutrient acquisition response” that enhances nutrients acquisition and assimilation (high P, Ca, and Mg accumulation) as well as an increase in the photochemical efficiency and activity of photosystem II (higher SPAD index). Foliar applications of the commercial PH decreased the polyphenols content, but on the other hand strongly increased total amino acid content (+45%, +82%, and +59% at 0, 15, and 30 kg ha−1, respectively) but not at a 45-kg ha−1-rate. Overall, the use of PH could represent a sustainable tool for boosting yield and nitrogen use efficiency and coping with soil fertility problems under low input regimens.

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An improved fluorimetric HPLC method for quantifying tocopherols in Brassica rapa L. subsp. sylvestris after harvest

Cited by 22 publications

References 20 publications

Durum Wheat Roots Adapt to Salinity Remodeling the Cellular Content of Nitrogen Metabolites and Sucrose

Durum Wheat Roots Adapt to Salinity Remodeling the Cellular Content of Nitrogen Metabolites and Sucrose

Durum wheat seedling responses to simultaneous high light and salinity involve a fine reconfiguration of amino acids and carbohydrate metabolism

Morphological and Physiological Responses Induced by Protein Hydrolysate-Based Biostimulant and Nitrogen Rates in Greenhouse Spinach

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