An improved in vitro model of human intestinal follicle-associated epithelium to study nanoparticle transport by M cells

Rieux, Anne des; Fiévez, Virginie; Théate, Ivan; Mast, Jan; Préat, Véronique; Schneider, Yves‐Jacques

doi:10.1016/j.ejps.2006.12.006

Cited by 276 publications

(219 citation statements)

References 43 publications

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“…The percentage of Raji cells in the culture assessed with flow cytometry was about 27 % of all cells; these data were consistent with the results published by Rieux et al [9], who obtained a percentage between 17 and 30% of total number of cells. Confocal images showed presence of CFSE labeled cells below the inserts (Fig.…”

Section: Localization and Quantification Of Raji Cells In Caco-2/rajisupporting

confidence: 92%

“…In vitro models were developed following a previous experiment by des Rieux et al [9]. The human colon adenocarcinoma cell line Caco-2 cells were seeded at a density of 9 x 10 4 cells on the apical Afterwards, Raji cells and silicon tubes were removed and inserts were placed in their original orientation.…”

Section: Preparation and Characterization Of Lipid Nanopartcilesmentioning

confidence: 99%

“…Several adapted in vitro models based on colorectal adenocarcinoma Caco-2 cells have been developed in the last decade to test drug permeability across the intestinal barrier [8,9,16,19]. Among all these models, Caco-2 monoculture and co-culture of Caco-2 cells with Burkitt lymphoma B cells (Raji cells)…”

Section: Transport Experimentsmentioning

confidence: 99%

“…Most authors support the need for direct contact between both cell lines in order to obtain a more reliable M-cell model [9,11]. In the inverted culture model, Raji cells are expected to migrate through the pores of the insert membrane and, therefore, to be incorporated into the Caco-2 cell monolayer.…”

Section: Localization and Quantification Of Raji Cells In Caco-2/rajimentioning

confidence: 99%

“…Previous authors have developed numerous strategies to obtain M-cell like in vitro models [8][9][10][11][12]. This work includes the development of an in vitro model of M cells based on the strategy of co-cultivating Caco-2 cells with Raji cells (B lymphocytes derived from Burkitt cell lymhoma) [9].…”

Section: Introductionmentioning

confidence: 99%

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Publisher¹

2014

CTMC

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Nanotechnology is providing a new therapeutic paradigm by enhancing drug efficacy and preventing side-effects. Edelfosine is a synthetic ether lipid analogue of platelet activating factor with high antitumor activity. The encapsulation of this potent antitumor drug in lipid nanoparticles increases its oral bioavailability; moreover, it prevents the hemolytic and gastrointestinal side-effects of the free drug. The literature points towards lymphatic absorption of lipid nanoparticles after oral administration, and previous in vitro and in vivo studies stress the protection against toxicity that these nanosystems provide.The present study is intended to assess the permeability of lipid nanoparticles across the intestinal barrier.Caco-2 monoculture and Caco-2/Raji co-culture were used as in vitro models of enterocytes and Microfold cells respectively. Results showed that free drug is internalized and possibly metabolized in enterocytes. These results do not correlate with those observed in vivo when edelfosine-lipid nanoparticles were administered orally in mice, which suggests that the microfold model is not a good model to study the absorption of edelfosine-lipid nanoparticles across the intestinal barrier in vitro.

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Section: Localization and Quantification Of Raji Cells In Caco-2/rajisupporting

confidence: 92%

Section: Preparation and Characterization Of Lipid Nanopartcilesmentioning

confidence: 99%