An improved method for murine laser-induced choroidal neovascularization lesion quantification from optical coherence tomography images

Jensen, Nathan R.; Lambert-Cheatham, Nathan; Hartman, Gabriella D.; Anbukkarasi, Muniyandi; Park, Bomina; Sishtla, Kamakshi; Corson, Timothy W.

doi:10.1016/j.mex.2022.101809

Cited by 1 publication

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“…The quantification of lesion volume from OCT images, after exclusion, was performed as previously reported. [52,53] Exclusion criteria include hemorrhage (diameter of bleeding area exceeding that of lesion), excessive laser burn, and fused lesions. Also, FA was performed at day 14 right after fundus and OCT imaging.…”

Section: Methodsmentioning

confidence: 99%

Long‐Acting Microparticle Formulation of Griseofulvin for Ocular Neovascularization Therapy

Chobisa,

Muniyandi,

Sishtla

et al. 2023

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Neovascular age‐related macular degeneration (nAMD) is a leading cause of vision loss in older adults. nAMD is treated with biologics targeting vascular endothelial growth factor; however, many patients do not respond to the current therapy. Here, a small molecule drug, griseofulvin (GRF), is used due to its inhibitory effect on ferrochelatase, an enzyme important for choroidal neovascularization (CNV). For local and sustained delivery to the eyes, GRF is encapsulated in microparticles based on poly(lactide‐co‐glycolide) (PLGA), a biodegradable polymer with a track record in long‐acting formulations. The GRF‐loaded PLGA microparticles (GRF MPs) are designed for intravitreal application, considering constraints in size, drug loading content, and drug release kinetics. Magnesium hydroxide is co‐encapsulated to enable sustained GRF release over >30 days in phosphate‐buffered saline with Tween 80. Incubated in cell culture medium over 30 days, the GRF MPs and the released drug show antiangiogenic effects in retinal endothelial cells. A single intravitreal injection of MPs containing 0.18 µg GRF releases the drug over 6 weeks in vivo to inhibit the progression of laser‐induced CNV in mice with no abnormality in the fundus and retina. Intravitreally administered GRF MPs prove effective in preventing CNV, providing proof‐of‐concept toward a novel, cost‐effective nAMD therapy.

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Section: Methodsmentioning

confidence: 99%