An improved micropropagation and ex vitro rooting of a commercially important crop Henna (Lawsonia inermis L.)

Abstract

An improved micropropagation protocol has been developed for a cosmetically important, dye yielding crop, henna (Lawsonia inermis). Quality of henna product is governed by naphthoquinone based pigment lawsone, thus in vitro multiplication of superior healthy plant to achieve enhanced productivity in terms of dye content and biomass deserve due attention. In the present study, nodal explants collected from an elite plant screened on the basis of superiority in lawsone content was cultured on MS medium supplemented with 0.5 lM benzyl adenine (BA) gave significantly (p \ 0.05) high number of shoots (24.33). The explants placed on MS medium augmented with 0.5 lM BA and 2-isopentenyladenine (2-iP) resulted in the formation of maximum number of shoots (43.67) and was elongated (12.57 cm) within 4 weeks of culture period. Enhanced axillary bud proliferation and production of mass number of micro shoots was achieved by the continuous subculture in MS medium containing 0.5 lM BA and 2-iP. In vitro raised micro shoots were dipped in 0.44 mM NAA for 5 min followed by planting in polyethylene pots containing a soil: vermiculite (1:1 v/v) mixture produced rooted plantlets (100%). Different auxin types and its concentrations had significant role rooting of L. inermis. Rooting response of various size shoots of L. inermis treated with 0.44 mM NAA showed 100% rooting in 4.1-5 cm size class shoots. After two months of potting, survived (95%) plants were successfully transferred to medicinal plant garden of the Department. The lawsone content of one-year-old micropropagated plants (23.04 mg/ g dw) growing in normal environmental conditions and elite mother plant (22.84 mg/g dw) was almost similar. Through the present study, efficient cloning of superior germplasm of L. inermis was established. Keywords Axillary bud Á Explant Á Ex vitro rooting Á Lawsonia inermis L. Á Micro-shoots Á MS medium

Stabilisation and detoxification of henna (Lawsonia inermis L.) extract for hair dye cosmetics by spray‐drying encapsulation

“…Henna (Lawsonia inermis L.) leaves are one of the main sources of natural hair dyes used in Asia and North Africa since antiquity, and are still extensively used today in a number of cosmetic applications including hair dyeing and skin painting. 1 The principal colouring agent in henna leaves is lawsone (2-hydroxy-1,4-naphthoquinone), also known as CI Natural Orange 6, which acts as a direct or mordant dye for hair fibre to give a red-orange colour. Besides hair dyeing, the interest in using henna for textile coloration has grown rapidly because of its green and ecofriendly nature.…”

High Efficiency Regeneration System from Blueberry Leaves and Stems

“…The study showed that 2.0 mg/L CPPU and 0.2 mg/L 2-ip can promote callus induction, and 3.0 mg/LCPPU can promote the growth of embryogenic callus in blueberry leaves ( Figure 1 and Figure 2 ; Table 9 and Table 10 ). In addition, CPPU [ 36 , 37 , 38 ] and 2iP [ 39 , 40 , 41 ] are active and promote bud growth in blueberries. In the present experiment, 1.0 mg/LCPPU and 0.4 mg/L 2-ip promote bud production and growth in blueberry, and the growth state of buds is better ( Figure 3 ; Table 11 ).…”

Germinación y multiplicación de plantas in vitro DE Heimia salicifolia (LYTHRACEAE)

“…Los antecedentes de éxito de protocolos similares en otras especies herbáceas y en otras especies de la familia Lythraceae como lo es Lawsonia inermis, validan el empleo de citocininas para la producción de brotes y nuevas plántulas para la micropropagación de Heimia salicifolia, justificando así su adecuación a la especie del presente trabajo. Se han reportado, efectos positivos de la actividad de BA como la citocinina más activa para la multiplicación de explantes en plantas herbáceas, como Cassia angustifolia (Husain & Anis, 2009), Melia azadirachta (Ram & Shekhawat, 2011) y en Lawsonia inermis (Shiji & Siril, 2018); motivo por el cual se decidió su implementación en este protocolo, junto con el resto de los reguladores de crecimiento.…”

“…10). El tratamiento dos es eficaz para la producción de hojas y numerosos brotes vigorosos, lo cual se traduce en mayor cantidad de peso fresco producido, a emplearse en pruebas futuras referentes a su producción de alcaloides; estas concentraciones y su uso también ha sido eficaz en la micropropagación de otras especies con potencial farmacéutico, como Sophora tonkinensis (Jana et al, 2013) y en miembros de la misma familia como Lawsonia inermis (Shiji & Siril, 2018). La producción de hojas y brotes idénticas en los explantes basales contra los apicales del tratamiento dos, se relaciona con la disrupción de la dominancia apical del meristemo, proveyendo por lo tanto de un sistema eficaz y consistente para micropropagación de Heimia salicifolia sin muestra de signos de variación genética, generando una clonación a partir de dos vías de explantes, con lo cual se logra la duplicación de plántulas provenientes de una sola semilla germinada.…”