An Improved Micropropagation Protocol byEx VitroRooting ofPassiflora edulisSims. f.flavicarpaDeg. through Nodal Segment Culture

Abstract

A procedure for rapid clonal propagation of Passiflora edulis Sims. f. flavicarpa Deg. (Passifloraceae) has been developed in this study. Nodal explants were sterilized with 0.1% HgCl2 and inoculated on Murashige and Skoog (MS) basal medium. The addition of 2.0 mgL−1 6-benzylaminopurine (BAP) to MS medium caused an extensive proliferation of multiple shoots (8.21 ± 1.13) primordial from the nodal meristems. Subculturing of these multiple shoots on the MS medium augmented with 1.0 mgL−1 of each BAP and Kinetin (Kin) was successful for the multiplication of the shoots in vitro with maximum numbers of shoots (25.73 ± 0.06) within four weeks of incubation. Shoots were rooted best (7.13 ± 0.56 roots/shoots) on half strength MS medium supplemented with 2.0 mgL−1 indole-3 butyric acid (IBA). All in vitro regenerated shoots were rooted by ex vitro method, and this has achieved 6-7 roots per shoot by pulsing of cut ends of the shoots using 200 as well as 300 mgL−1 IBA. The plantlets were hardened in the greenhouse for 4-5 weeks. The hardened plantlets were shifted to manure containing nursery polybags after five weeks and then transferred to a sand bed for another four weeks for acclimatization before field planting with 88% survival rate.

Assessment of minimum growth conditions for in vitro conservation of Passiflora edulis Sims

“…Although acclimatization is a very sensitive stage in in vitro culture of plants, in some passion fruit genotypes, this stage has been successfully carried out (Isutsa, 2004;Shekhawat et al, 2015). According to Tanno and Biasi (2013), grapevines were efficiently acclimatized in MS medium with increasing doses of sucrose.…”

Preculturing effect of thidiazuron onin vitroshoot multiplication and micropropagation round inCapparis decidua(Forsk.) an important multipurpose plant

“…Of the different concentrations of IBA tested, the maximum frequency of root formation (84%), number of roots (5.7 ± 0.41) and root length (4.4 ± 0.39 cm) was achieved in 200 µM IBA (Table 3). Ex vitro rooting was also found to be effective in Tylophora indica [9], Cyamopsis tetragonoloba [1], Cassia angustifolia [24], Passiflora edulis [23]. The advantage of this method is that rooting and acclimatization was done simultaneously.…”

In vitro propagation of Passiflora edulis through internodal segments as affected by medium composition

“…For shoot formation from thin cell layer explants of P. edulis, 1.0 mg L -1 BA was suggested in the study by Nhut et al (2007). Jafari et al (2017) reported that the combination of 1.5 mg L -1 BAP and 0.15 mg L -1 IBA promoted the highest regeneration frequency with the highest number of shoots from cotyledonary node and shoot tip explants of P. caerulea, while semi-solid MS medium supplemented with 2.0 mg L -1 BAP was found to be most appropriate for shoot formation from nodal shoot meristems of P. foetida (Shekhawat et al, 2015 a).…”

“…Because of these problems, it is important to find the most efficient way to propagate P. edulis through in vitro culture for the micropropagation of true-to-type plant regenerants from elite plants. In vitro micropropagation of the Passifloraceae family has been reported for some species such as Passiflora alata (Pinto et al, 2010), P. caerulea (Jafari et al, 2017) and P. foetida (Shekhawat et al, 2015 a). In vitro regeneration of P. edulis has been obtained from internodal segments (Biasi et al, 2000), leaf segments (Trevisan, Mendes, 2005), hypocotyl explants (Fernando et al, 2007), apical meristem (Prammanee et al, 2011) androot (da Silva et al, 2011) and nodal (Shekhawat et al, 2015 b) segments.…”