An improved surface disinfection method for shoot expiants fromIrisrhizomes infected with bacterial soft rot (Erwinia carotovorasubsp,carotovora)

Berger, F.; Waites, W. M.; Leifert, Carlo

doi:10.1080/14620316.1994.11516480

Cited by 5 publications

(4 citation statements)

References 4 publications

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“…Although tissue culture propagation of Irises has been accomplished with various explants, continuous availability of the source of explant and its aseptic nature, and ability to respond to induction and propagation methods are, however, crucial for the successful establishment of a regeneration protocol 6 , 10 , 12 . Disinfection of the source material prior to inoculation or isolation of explant is essential as microorganisms that reside on the explant surface may find the in vitro environment optimal for their growth and affect the overall success of tissue culture 6 , 10 , 23 . Direct contact of the explants with disinfectants, in order to fully eliminate the contaminants, can severely affect their regeneration potency 10 .…”

Section: Discussionmentioning

confidence: 99%

“…Sterilization with 75% EtOH treatment increases permeability, and prolonged soaking can result in decolorization, injury and toxicity, as in Iris ensata 8 , 24 . Depending on the likely microbial contaminants, several surface disinfectants such as ethanol, NaOCl, H 2 O 2, AgNO 3, HgCl 2 , and bromine water are commonly used for surface sterilization, prior to in vitro culture 23 , 25 , 26 .…”

Section: Discussionmentioning

confidence: 99%

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Establishment of an efficient in vitro propagation system for Iris sanguinea

Wang

Rahman

et al. 2018

Sci Rep

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Iris sanguinea is a perennial flowering plant that is typically cultivated through seeds or bulbs. However, due to limitations in conventional propagation, an alternate regeneration system using seeds was developed. The protocol included optimization of sterilization, stratification and scarification methods as iris seeds exhibit physiological dormancy. In addition to chlorine-based disinfection, alkaline or heat treatment was used to break seed dormancy and reduce contamination. When seeds were soaked in water at 80 °C overnight, and sterilized with 75% EtOH for 30 s and 4% NaOCl solution for 20 minutes, contamination was reduced to 10% and a 73.3% germination was achieved. The germinated seedlings with 2-3 leaves and radicle were used as explants to induce adventitious buds. The optimal MS medium with 0.5 mg L−1 6-benzylaminopurine, 0.2 mg L−1 NAA, and 1.0 mg L−1 kinetin resulted in 93.3% shoot induction and a proliferation coefficient of 5.30. Medium with 0.5 mg L−1 NAA achieved 96.4% rooting of the adventitious shoots. The survival rate was more than 90% after 30 days growth in the cultivated matrix. In conclusion, a successful regeneration system for propagation of I. sanguinea was developed using seeds, which could be utilized for large-scale propagation of irises of ecological and horticultural importance.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Establishment of an efficient in vitro propagation system for Iris sanguinea

Wang

Rahman

et al. 2018

Sci Rep

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“…Contaminants may be introduced with the expant, during manipulations in the laboratory and/or by micro-arthropod vectors (Cassells, 1991, in press a; Leifert et al, 1991aLeifert et al, , 1994aBerger et al, 1994). Gram-negative bacteria such as Pseudomonas (in particular In Vitro Cell.…”

Section: Identity Sources and Epidemiology Of Contaminationmentioning

confidence: 99%

Microbial hazards in plant tissue and cell cultures

Leifert

Cassells

2001

In Vitro Cell.Dev.Biol.-Plant

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171

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“…Sixteen strains of Lactobacillus plantarum isolated from shoot cultures of the Hemerocallis varieties 'Stafford', 'Catherine Woodbury' and 'Stella d'ora' which had been micropropagated in vitro for longer than 36 months and 14 strains from surface disinfected (protocol 1; Berger et al 1994) stem sections of glasshouse grown plants from the same varieties were compared in this study. All strains were isolated from stock plants or tissue cultures in the same micropropagation laboratory.…”

Section: Bacterial Stralnsmentioning

confidence: 99%

Plasmid profiles of Lactobacillus plantarum spp. found as contaminants in Hemerocallis plant tissue cultures

Leifert

Berger

Stewart

et al. 1994

Lett Appl Microbiol

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Plasmid profiles of 30 strains of Lactobacillus plantarum isolated from 3‐year‐old tissue cultures and surface sterilized stem sections of glasshouse grown plants of three different varieties of Hemerocallis were compared to pinpoint the source of Lact. plantarum contamination in plant tissue cultures. Since the profiles of strains isolated from in vitro and in vivo plant material were generally identical, it is thought that the source of Lact. plantarum contamination is the plant material used to initiate plant tissue cultures.

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An improved surface disinfection method for shoot expiants fromIrisrhizomes infected with bacterial soft rot (Erwinia carotovorasubsp,carotovora)

Cited by 5 publications

References 4 publications

Establishment of an efficient in vitro propagation system for Iris sanguinea

Establishment of an efficient in vitro propagation system for Iris sanguinea

Microbial hazards in plant tissue and cell cultures

Plasmid profiles of Lactobacillus plantarum spp. found as contaminants in Hemerocallis plant tissue cultures

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