An Improved Synthetic Medium for Oospore Production and Germination of Several Phytophthora Species

Ribeiro, O. K.; Erwin, D. C.; Zentmyer, G. A.

doi:10.2307/3758592

Cited by 29 publications

(15 citation statements)

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“…The percentage of oospore germination was used as an evaluation indicator for estimating the survival of oospores in soils. However, some studies showed that the ratio of oospore germination in the case of P. capsici (heterothallic) was very low, ranging from 0 to 51% (Satour and Butler, 1968;Ribeiro et al, 1975;Boccas, 1981;Papavizas et al, 1981;Hord and Ristaino, 1991). The ratio of low germination maybe attributed to some complicated reasons, such as oospore dormancy and abnormal development (Etxeberria et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Antagonistic interaction between Trichoderma asperellum and Phytophthora capsici in vitro

Jiang

Zhang

et al. 2016

J. Zhejiang Univ. Sci. B

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Phytophthora capsici is a phytopathogen that causes a destructive pepper blight that is extremely difficult to control. Using a fungicide application against the disease is costly and relatively ineffective and there is also a huge environmental concern about the use of such chemicals. The genus Trichoderma has been known to have a potential biocontrol issue. In this paper we investigate the mechanism for causing the infection of T. asperellum against P. capsici. Trichoderma sp. (isolate CGMCC 6422) was developed to have a strong antagonistic action against hyphae of P. capsici through screening tests. The strain was identified as T. asperellum through using a combination of morphological characteristics and molecular data. T. asperellum was able to collapse the mycelium of the colonies of the pathogen through dual culture tests by breaking down the pathogenic hyphae into fragments. The scanning electron microscope showed that the hyphae of T. asperellum surrounded and penetrated the pathogens hyphae, resulting in hyphal collapse. The results show that seven days after inoculation, the hyphae of the pathogen were completely degraded in a dual culture. T. asperellum was also able to enter the P. capsici oospores through using oogonia and then developed hyphae and produced conidia, leading to the disintegration of the oogonia and oospores. Seven days after inoculation, an average 10.8% of the oospores were infected, but at this stage, the structures of oospores were still intact. Subsequently, the number of infected oospores increased and the oospores started to collapse. Forty-two days after inoculation, almost all the oospores were infected, with 9.3% of the structures of the oospores being intact and 90.7% of the oospores having collapsed.

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Section: Discussionmentioning

confidence: 99%

Antagonistic interaction between Trichoderma asperellum and Phytophthora capsici in vitro

Jiang

Zhang

et al. 2016

J. Zhejiang Univ. Sci. B

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“…Ribeiro's defined medium (Ribeiro et a/., 1965) as modified by Fenn & Coffey (1984) was used for mycelial production, phosphate concentrations being adjusted as required. As the uptake of phosphite and the degree of growth inhibition have been shown to be sensitive to external phosphate concentrations (J. M. Griffith, unpublished results), mycelia were grown in both highphosphate and low-phosphate media.…”

Section: A) 1984)mentioning

confidence: 99%

31P NMR studies on the effect of phosphite on Phytophthora palmivora

Niere¹,

Griffith

Grant

1990

Journal of General Microbiology

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31P N M R spectra were obtained from perchloric acid (PCA) and KOH extracts of Phytophthora pdmivora mycelium. Signals indicating the presence of large amounts of short-chain plyphosphate were observed in the spectra of PCA extracts of mycelia grown under both low (0.1 mM) and high (10 mM) phosphate conditions. The mean chain length of polyphosphate was calculated from the relative areas of signals arising from terminal and internal P nuclei in the polyphosphate chain. The small amount of plyphosphate evident in the KOH extract had an average chain length similar to PCA-soluble polyphosphate. 32P tracer studies indicated that phosphorus in the PCA fraction accounted for between 50 and 60% of total phosphorus, the bulk of the remainder being divided between the lipid and KOH extracts. The presence of the fungicide phosphorous acid markedly reduced the average chain length of acid-soluble polyphosphate. This reduction occurred both under low-phosphate conditions, in which treatment with phosphorous acid retards growth, and under high-phosphate conditions, in which no significant growth retardation is observed. Treatment with phosphorous acid perturbed phosphorus distribution and lipid composition under low-phosphate conditions.

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“…However, it has long been known that the mating type reaction in Phytophthora is non-specific, Al types of one species often reacting with A2 types of a different species (Stamps, 1953 ;Haasis & Nelson, I 963 ;Ribeiro, Erwin & Zentmyer, 1975). In such reactions where both parents produce both oogonia and antheridia, there may be reciprocal hybrid gametangial combinations and also a certain proportion of selfed gametangial combinations.…”

Section: Introductionmentioning

confidence: 99%

Polyploidy and Induced Gametangial Formation in British Isolates of Phytophthora infestans

Sansome¹

1977

Journal of General Microbiology

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SUMMARYGametangial (sex organ) formation in cultures of a British isolate of Phytophthora infestans, mating type Al, was induced by simultaneous inoculation with both Al and A2 isolates of P. palmivora. Inoculation with the A2 mating type only was ineffective. Three isolates of P. infestans were examined: two were polyploid, of which one was probably tetraploid; the third had both diploid and polyploid nuclei.Phytophthora infestans also produced gametangia when inoculated simultaneously with both Al and A2 types of P. cambivora and P. cinnumomi. The ecological significance of these findings is discussed.

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An Improved Synthetic Medium for Oospore Production and Germination of Several Phytophthora Species

Cited by 29 publications

References 0 publications

Antagonistic interaction between Trichoderma asperellum and Phytophthora capsici in vitro

Antagonistic interaction between Trichoderma asperellum and Phytophthora capsici in vitro

31P NMR studies on the effect of phosphite on Phytophthora palmivora

Polyploidy and Induced Gametangial Formation in British Isolates of Phytophthora infestans

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