An improved system to generate recombinant canine distemper virus

Abstract

Background Canine distemper virus (CDV) is a pathogen with the capability of cross-species transmission. It has crossed the species barrier to infect many other species, and its host range is expanding. The reverse genetic platform, a useful tool for scientific research, allows the generation of recombinant viruses from genomic cDNA clones in vitro. Methods To improve the reverse genetic system of CDV, a plasmid containing three independent expression cassettes was constructed for co-expression of the N, P, and L genes and then transfected with a full-length cDNA clone of CDV into Vero cells. Results The results indicated that the established rescue system has the advantages of being more convenient, easy to control the transfection ratio, and high rescue efficiency compared with the conventional reverse genetics system. Conclusion This method not only reduces the number of transfection plasmids, but also improves the rescue efficiency of CDV, which could provide a reference for the recovery of other morbilliviruses.

Correction: An improved system to generate recombinant canine distemper virus

“…Following the publication of the original article [ 1 ], it was noted that due to a typesetting error, the co-corresponding author name “Jingqiang Ren”, as well as the author’s email address “rjq207@163.com”, was not listed under the correspondence information field. The original article has been corrected.…”