An improvised shoot amplification and ex vitro rooting method for offsite propagation of Tinospora cordifolia (Willd.) Miers: a multi-valued medicinal climber

Panwar, Deepak; Patel, Ashok Kumar; Shekhawat, N. S.

doi:10.1007/s40502-018-0350-3

Cited by 13 publications

(5 citation statements)

References 36 publications

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“…(2018) for Tinospora cordifolia, Jaime et al (2018) for Smoke tree propagation has also shown such results [18,19]. Thus in the present study, by optimizing the concentration of IBA rooting was successfully obtained in the female shoots of G. indica plants.…”

Section: Ex-vitro Rootingsupporting

confidence: 67%

Optimization of IBA for Efficient Survival of Micropropagated Garcinia indica Choisy Shoots Using in-vitro and ex-vitro Rooting Techniques

Mulik

Manvi

Ingale

et al. 2019

APRJ

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Aims: The study focuses on optimization of concentration and time of rooting hormone exposure of IBA (Indole-3-Butyric Acid) for efficient survival of tissue culture raised Garcinia indica Choisy plantlets for in-vitro and ex-vitro rooting techniques. Study Design: The subcultured microshoots of Garcinia indica were subjected to in-vitro and ex-vitro rooting trials by treating them with IBA of varying concentration and time, to standardize these particular parameters required by this auxin to induce rooting. Place and Duration of Study: Rooting trials were carried out in Plant tissue culture-Biochemical Sciences Division of CSIR- National Chemical Laboratory, Pune 411008 between June 2018 and April 2019. Methodology: Regularly subcultured five to six years old shoots from female trees of Garcinia indica were used for the study. Various concentrations of IBA in correlation with time were used for in-vitro and ex-vitro root induction. The rooted plantlets were then transferred to polyhouse for acclimatization and will further be planted in open field locations in June 2019. Results: Induction of rooting was observed within thirty days of treatment with IBA. It was observed that 500ppm of IBA gave 30% rooting for in-vitro rooting trials whereas 2000ppm of IBA induced 80% rooting for shoots given ex-vitro rooting treatments. An interesting phenomenon that was observed for 70% of the shoots which failed to induce rooting by in-vitro treatment was that they survived with 100% rooting success under ex-vitro rooting conditions. The hardened plantlets were successfully acclimatized in the polyhouse with survival rate of 90% and were further transferred to polythene bags with rooting mixtures of sand: farmyard manure: coco peat (1:1:2). These plantlets have been healthy for the last 6 to 9 months and will be transported for field trials in June 2019. Conclusion: Ex-vitro rooting technique was found to be more effective than in-vitro rooting. Thus, by optimizing the rooting hormone parameters, female plants of Garcinia indica can be successfully grown using tissue culture technology and can be propagated in large numbers to increase the female plant number in plantations.

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Section: Ex-vitro Rootingsupporting

confidence: 67%

Optimization of IBA for Efficient Survival of Micropropagated Garcinia indica Choisy Shoots Using in-vitro and ex-vitro Rooting Techniques

Mulik

Manvi

Ingale

et al. 2019

APRJ

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“…This auxin-cytokinin synergism is known to control various significant developmental activities, including the formation and maintenance of meristem that are essential for shoot branching/regeneration (Su et al 2011). Similar synergism of auxin and cytokinin(s) for efficient shoot amplification has been reported in Salvadora oleoides (Phulwaria et al 2014), Tinospora cordifolia (Panwar et al 2018), and Moringa oleifera (Gupta et al 2020). The replacement of sucrose with table sugar did not affect the multiplication rate/response in this study as evidenced by Chhajer and Kalia (2017).…”

Section: Shoot Multiplication and Maintenance (I) Recurrent Passage Osupporting

confidence: 82%

“…Explants collected during the spring season were better in terms of percentage bud-breaking response, early bud-breaking, or days to respond and showed the least contamination in this study. Similarly, season-specific culture responses were earlier observed in Tecomella undulata (Chhajer and Kalia 2017) and Tinospora cordifolia (Panwar et al 2018). Funada et al (2001) explained that during the spring season, more auxins are synthesized in young buds which trigger cellular division in the cambium and hence assist in enhancing active growth.…”

Section: Resultsmentioning

confidence: 66%

“…Initial incubation in dark for 2 to 3 d also assisted in reducing phenolic exudation to an extent. Additionally, such initial exposure of explants to dark/diffused light conditions for a certain period can promote morphogenesis by axillary meristem activation as reported by Panwar et al (2018).…”

Section: Resultsmentioning

confidence: 76%

“…These steps are believed to be the most labor/resource intensive and determine the eventual success of a clonal propagation. It is estimated that CEVRA can reduce up to 30 to 70% cost of a micropropagation protocol and maximize the transplant survival rate in the field (Patel et al 2016b;Panwar et al 2018).…”

Section: Shoot Multiplication and Maintenance (I) Recurrent Passage Omentioning

confidence: 99%

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An improved micropropagation protocol for the ex situ conservation of Mitragyna parvifolia (Roxb.) Korth. (Rubiaceae): an endangered tree of pharmaceutical importance

Patel

Lodha

Shekhawat

2020

In Vitro Cell.Dev.Biol.-Plant

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Mitragyna parvifolia (Roxb.) Korth., commonly known as "Kadam," is an endangered and pharmaceutically valued tree of the family Rubiaceae. The numerous medicinal properties are attributed to the various alkaloids of this plant. Poor seedling survival (due to very small size of seeds, approximately 10,000 per gm), overexploitation and habitat destruction are the major constraints in conserving the wild stocks of this species. This paper reports a significant, improved, and repeatable micropropagation protocol of M. parvifolia using nodal explants of a mature tree. Nodal explants harvested during spring season from the lopped tree differentiated the maximum number of axillary shoots (5.3 ± 0.82 per node) on full-strength Murashige and Skoog (MS) medium containing 3.0 mg L −1 6-benzylaminopurine (BAP) and additives (25 mg L −1 each of adenine sulfate, L-arginine, and citric acid and 50 mg L −1 ascorbic acid). Shoots were amplified in vitro through (1) recurrent transfer of mother explants and (2) subculturing on fresh nutrient medium. The greatest number of shoots (13.4 ± 1.26) with an average length of 6.2 ± 1.03 cm was produced after 4 wk on MS medium containing 0.5 mg L −1 BAP, 0.25 mg L −1 kinetin (Kin), 0.1 mg L −1 Indole-3-acetic acid (IAA), additives, 100 mg L −1 activated charcoal (AC), and 0.8% (w/v) agar. This is the first report of concurrent ex vitro rooting and acclimatization (CEVRA) in M. parvifolia. About 90% micropropagated shoots rooted ex vitro on pulse treatment of 500 mg L −1 Indole-3-butyric acid (IBA; for 5 min) and produced 8.5 ± 0.97 roots per shoot with an average length of 9.40 ± 1.06 cm, after 5 wk. Over 80% of CEVRA plantlets were successfully transplanted to the soil in field. The defined protocol can be employed for conservation ex situ and restoration/rehabilitation/reintroduction in situ of M. parvifolia. Keywords Endangered tree. Ex situ conservation. Ex vitro rooting. Micropropagation. Mitragyna parvifolia This paper is dedicated to the frontline workers of COVID-19 Pandemic.

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Investigating the Use of Biotechnologically Enhanced Medicinal Plants in Allergy Treatment

Mukherjee,

Dave,

Panigrahi

et al. 2024

Biotechnology of Medicinal Plants With Antiallergy Properties

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An improvised shoot amplification and ex vitro rooting method for offsite propagation of Tinospora cordifolia (Willd.) Miers: a multi-valued medicinal climber

Cited by 13 publications

References 36 publications

Optimization of IBA for Efficient Survival of Micropropagated Garcinia indica Choisy Shoots Using in-vitro and ex-vitro Rooting Techniques

Optimization of IBA for Efficient Survival of Micropropagated Garcinia indica Choisy Shoots Using in-vitro and ex-vitro Rooting Techniques

An improved micropropagation protocol for the ex situ conservation of Mitragyna parvifolia (Roxb.) Korth. (Rubiaceae): an endangered tree of pharmaceutical importance

Investigating the Use of Biotechnologically Enhanced Medicinal Plants in Allergy Treatment

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