An In-Vitro Assay Estimating Changes in Melanin Content of Melanoma Cells due to Ultra-Dilute, Potentized Preparations

Abstract

Introduction The authors had previously conducted an in-vitro study to observe the effect of homeopathic medicines on melanogenesis, demonstrating anti-vitiligo potential by increasing the melanin content in murine B16F10 melanoma cells. A similar experiment was performed using further homeopathic preparations sourced from kojic acid (KA), hydrogen peroxide (H2O2; HP), 6-biopterin (BP), and [Nle4, D-Phe7]-α-melanocyte-stimulating hormone (NLE), some of which are known to induce vitiligo or melano-destruction at physiological dose. Materials and Methods The homeopathic preparations of BP, KA, NLE, and HP were used in 30c potency. Alcohol and potentized alcohol were used as vehicle controls. Prior to starting the main experiment, the viability of B16F10 melanoma cells after treatment with study preparations was assayed. Melanin content (at 48 h and 96 h) and tyrosinase activity in melanocytes were determined. Results At the end of 48 hours, NLE and HP in 30c potency had a significantly greater melanin content (p = 0.015 and p = 0.039, respectively) compared with controls; BP and KA in 30c potency had no significant effects. No significant changes were seen at the end of 96 hours. KA, NLE, HP, and vehicle controls showed an inhibition of tyrosinase activity. Conclusion The study demonstrated melanogenic effects of two homeopathic preparations. Further research to evaluate the therapeutic efficacy of these medicines is warranted.

Homeopathy in Experimental Cancer Models: A Systematic Review

“…Finally, the two studies by Munshi et al 27,28 do not strictly address homeopathy in cancer, but used melanoma cells in the attempt to understand the mechanisms underlying homeopathic treatment of vitiligo. For this reason, we do not discuss them further here.…”

“…59 From this perspective, using both succussed and non-succussed vehicles may represent a good standard for controls in future studies in vitro. As shown in ►Table 3, only two studies used both types 27,28 whilst most only used vehicle and untreated cells, following the standards of conventional pharmacological research.…”

“…vitro. As shown in ►Table 3, only two studies used both types 27,28 whilst most only used vehicle and untreated cells, following the standards of conventional pharmacological research.…”

Atenção Farmacêutica Na Homeopatia

“…A Homeopatia tem favorecido a melhora da saúde pública, já que apresentou estudos envolvendo resultados positivos em uso de doses ultra baixas de ácido acetil salicílico em tromboses e hemorragias (EIZAYAGA et al, 2019), controle e manutenção de doenças epidêmicas (JACOBS, 2018), no controle de infestação de larvas de Cochliomyia hominivorax (Miíase) (DE BARROS et al, 2019), na indução de melanogênese no vitiligo (MUNSHI et al, 2019), prognóstico de pacientes infectados com Encephalitozoon cuniculi (NAGAI et al, 2019).…”