An in vitro biofilm model associated to dental implants: Structural and quantitative analysis of in vitro biofilm formation on different dental implant surfaces

Sanchez, Maria Carmen Arroyo; Llama‐Palacios, Arancha; Fernández, E; Figuero, Elena; Marín, Ma José; León, Rubén; Blanc, Vanessa; Herrera, David; Sanz, Mariano

doi:10.1016/j.dental.2014.07.008

Cited by 101 publications

(103 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The biofilm formation in vivo takes longer time than in vitro, which may be due to the “race to surface” between bacteria and host cells. Bacterial attachment and biofilm formation stage lasted 12–24 h, and the biofilm proliferation and maturation lasted 36–72 h for completion [43, 44]. The early infection may be defined up to 3–4 weeks during which debridement and antibiotic therapy with the retained stable implants were performed in the traditional management perspective [45, 46].…”

Section: Mechanism Of the Iris—biofilmmentioning

confidence: 99%

Coatings as the useful drug delivery system for the prevention of implant-related infections

Pan

Zhou

2018

J Orthop Surg Res

View full text Add to dashboard Cite

Implant-related infections (IRIs) which led to a large amount of medical expenditure were caused by bacteria and fungi that involve the implants in the operation or in ward. Traditional treatments of IRIs were comprised of repeated radical debridement, replacement of internal fixators, and intravenous antibiotics. It needed a long time and numbers of surgeries to cure, which meant a catastrophe to patients. So how to prevent it was more important than to cure it. As an excellent local release system, coating is a good idea by its local drug infusion and barrier effect on resisting biofilms which were the main cause of IRIs. So in this review, materials used for coatings and evidences of prevention were elaborated.

show abstract

Section: Mechanism Of the Iris—biofilmmentioning

confidence: 99%

Coatings as the useful drug delivery system for the prevention of implant-related infections

Pan

Zhou

2018

J Orthop Surg Res

View full text Add to dashboard Cite

show abstract

“…51 The sterile saliva pellicle-coated resin disks were transferred to a new 24-well plate. Each well was inoculated with 1.5 mL mixed bacterial suspension and incubated in anaerobic conditions (90% N 2 , 5% CO 2 , 5% H 2 ) at 37°C for 72 h, and the medium was changed every 24 h. 53 Plates containing only culture medium were also incubated to check for sterility. This totaled 3 days of culture, which was shown to form periodontal biofilms on resins.…”

Section: Methodsmentioning

confidence: 99%

Novel multifunctional dental bonding agent for class-V restorations to inhibit periodontal biofilms

Wang

Weir

et al. 2017

RSC Adv.

View full text Add to dashboard Cite

We recently developed a dental bonding agent to bond restorations to teeth using nanoparticles of amorphous calcium phosphate (NACP) for remineralization with rechargeable calcium and phosphate ion release. The objectives of this study were to: (1) incorporate an antibacterial monomer dimethylaminohexadecyl methacrylate (DMAHDM) and a protein-repellent agent 2-methacryloyloxyethyl phosphorylcholine (MPC); and (2) investigate protein adsorption and periodontitis-related biofilms for the first time. A primer, used to prime tooth structures for bonding, was made with pyromellitic glycerol dimethacrylate (PMGDM) and 2-hydroxyethyl methacrylate (HEMA). An adhesive was made with PMGDM, ethoxylated bisphenol A dimethacrylate and HEMA. NACP, MPC and DMAHDM were incorporated. Streptococcus gordonii, Actinomyces naeslundii, Porphyromonas gingivalis, Fusobacterium nucleatum were cultured to form single and multi-species biofilms. Colony-forming units (CFU), live/dead, metabolic activity, and polysaccharide were measured. Adding DMAHDM, MPC and NACP into the bonding agent did not compromise the dentin bond strength (p > 0.1). Bonding agents with 5% MPC reduced protein adsorption to 1/15 that of the control (p < 0.05). Bonding agents with 5% DMAHDM + 5% MPC had much greater reduction in biofilms than DMAHDM or MPC alone (p < 0.05). Biofilm CFU was reduced by 3 to 4 log via DMAHDM + MPC. Metabolic activities and polysaccharide of biofilms were also substantially reduced (p < 0.05). In conclusion, a novel bonding agent was developed for dental restorations with inhibition of biofilms, reducing CFU by 3 to 4 log. Besides remineralizartion and acid-neutralization via NACP to inhibit caries as shown previously, the multifunctional adhesive is promising for root restorations with subgingival margins to suppress periodontal pathogens and protect the periodontium.

show abstract

“…Many researchers (see, for example, [4,10,15]) report that HA has antibacterial properties, but others report that HA favors the formation of bacterial biofilm in the vicinity of an implant causing peri-implantitis and implant failures [16]. We report here some results of an in vitro comparison of the effect on Staphylococcus epidermidis of an unprocessed titanium alloy vs. titanium oxide surface layer vs. hydroxyapatite produced on the alloy by Plasma Electrolytic Oxidation (PEO) with subsequent hydrothermal treatment [11] at various pH values and processing times.…”

Section: Introductionmentioning

confidence: 99%

Morphology and antimicrobial properties of hydroxyapatite–titanium oxide layers on the surface of Ti–6Al–4V alloy

Lugovskoy

Weiss

Tsadok

et al. 2016

Surface and Coatings Technology

View full text Add to dashboard Cite

An in vitro biofilm model associated to dental implants: Structural and quantitative analysis of in vitro biofilm formation on different dental implant surfaces

Cited by 101 publications

References 31 publications

Coatings as the useful drug delivery system for the prevention of implant-related infections

Coatings as the useful drug delivery system for the prevention of implant-related infections

Novel multifunctional dental bonding agent for class-V restorations to inhibit periodontal biofilms

Morphology and antimicrobial properties of hydroxyapatite–titanium oxide layers on the surface of Ti–6Al–4V alloy

Contact Info

Product

Resources

About