An in vitro chemosensitivity test for gastric cancer using collagen gel droplet embedded culture.

Hanatani, Yuji; Kobayashi, Hisayuki; Kodaira, Susumu; Takami, Hideki; Asagoe, Tatsuo; Kaneshiro, Edna S.

doi:10.3892/or.7.5.1027

Cited by 11 publications

(10 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The efficacy of the CD-DST has been clinically shown in several types of cancer. For example, Hanatani et al [17] reported promising data with the CD-DST for gastric cancer. In addition, Takamura et al [18] showed the CD-DST had good predictive value for the clinical response in breast cancer patients, especially for chemotherapy using TXT or some combined anticancer drugs.…”

Section: Discussionmentioning

confidence: 97%

Prediction of chemotherapeutic effect on postoperative recurrence by in vitro anticancer drug sensitivity testing in non-small cell lung cancer patients

et al. 2010

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 97%

Prediction of chemotherapeutic effect on postoperative recurrence by in vitro anticancer drug sensitivity testing in non-small cell lung cancer patients

et al. 2010

View full text Add to dashboard Cite

“…This is followed by culture for 1 week in culture medium not containing anti-cancer agents, and the number of cells indicates the degree of suppression of cell proliferation by each agent [12][13][14][15]. In the modified CD-DST method we used, the incubator temperature was set at 42 C for the first hour of the 24-h drug exposure period.…”

Section: Discussionmentioning

confidence: 99%

“…Denaturation of the collagen gel does not occur at 42 C, the same temperature used for thermotherapy, so we used the CD-DST method [12][13][14][15][16][17] for our in vitro chemosensitivity analysis of the anti-cancer agents, and investigated whether this method can be used for the selection of the thermochemotherapy regimen, instead of in vivo testing using nude mice.…”

Section: Introductionmentioning

confidence: 99%

Prediction of anti-tumour effect of thermochemotherapy within vitrothermochemosensitivity testing for non-small cell lung cancer

Abiko

Kawamura

Izumi

et al. 2007

International Journal of Hyperthermia

View full text Add to dashboard Cite

show abstract

“…CD-DST has little effect on non-cancerous cells, allowing accurate measurements of cancerous cells only (14)(15)(16). This method has been used chiefly on tumors of the digestive system (17,18). Compared to such cancers of the primary organs, oral squamous cell carcinoma (OSCC) and other oral cancers generally have a smaller tumor volume.…”

Section: Introductionmentioning

confidence: 99%

Collagen gel droplet-embedded culture drug sensitivity testing in squamous cell carcinoma cell lines derived from human oral cancers: Optimal contact concentrations of cisplatin and fluorouracil

2016

View full text Add to dashboard Cite

Abstract. The collagen gel droplet-embedded culture drug sensitivity test (CD-DST) is an anticancer drug sensitivity test that uses a method of three-dimensional culture of extremely small samples, and it is suited to primary cultures of human cancer cells. It is a useful method for oral squamous cell carcinoma (OSCC), in which the cancer tissues available for testing are limited. However, since the optimal contact concentrations of anticancer drugs have yet to be established in OSCC, CD-DST for detecting drug sensitivities of OSCC is currently performed by applying the optimal contact concentrations for stomach cancer. In the present study, squamous carcinoma cell lines from human oral cancer were used to investigate the optimal contact concentrations of cisplatin (CDDP) and fluorouracil (5-FU) during CD-DST for OSCC. CD-DST was performed in 7 squamous cell carcinoma cell lines derived from human oral cancers (Ca9-22, HSC-3, HSC-4, HO-1-N-1, KON, OSC-19 and SAS) using CDDP (0.15, 0.3, 1.25, 2.5, 5.0 and 10.0 µg/ml) and 0.9, 1.8, 3.8, 7.5,15.0 and 30.0 µg/ml), and the optimal contact concentrations were calculated from the clinical response rate of OSCC to single-drug treatment and the in vitro efficacy rate curve. The optimal concentrations were 0.5 µg/ml for CDDP and 0.7 µg/ml for 5-FU. The antitumor efficacy of CDDP at this optimal contact concentration in CD-DST was compared to the antitumor efficacy in the nude mouse method. The T/C values, which were calculated as the ratio of the colony volume of the treatment group and the colony volume of the control group, at the optimal contact concentration of CDDP and of the nude mouse method were almost in agreement (P<0.05) and predicted clinical efficacy, indicating that the calculated optimal contact concentration is valid. Therefore, chemotherapy for OSCC based on anticancer drug sensitivity tests offers patients a greater freedom of choice and is likely to assume a greater importance in the selection of treatment from the perspectives of function preservation and quality of life, as well as representing a treatment option for unresectable, intractable or recurrent cases.

show abstract

An in vitro chemosensitivity test for gastric cancer using collagen gel droplet embedded culture.

Cited by 11 publications

References 0 publications

Prediction of chemotherapeutic effect on postoperative recurrence by in vitro anticancer drug sensitivity testing in non-small cell lung cancer patients

Prediction of chemotherapeutic effect on postoperative recurrence by in vitro anticancer drug sensitivity testing in non-small cell lung cancer patients

Prediction of anti-tumour effect of thermochemotherapy within vitrothermochemosensitivity testing for non-small cell lung cancer

Collagen gel droplet-embedded culture drug sensitivity testing in squamous cell carcinoma cell lines derived from human oral cancers: Optimal contact concentrations of cisplatin and fluorouracil

Contact Info

Product

Resources

About