An In Vitro Chondrocyte Electrical Stimulation Framework: A Methodology to Calculate Electric Fields and Modulate Proliferation, Cell Death and Glycosaminoglycan Synthesis

Vaca‐González, Juan Jairo; Guevara, Johana; Vega, J. F.; Garzón‐Alvarado, Diego Alexander

doi:10.1007/s12195-015-0419-2

Cited by 30 publications

(56 citation statements)

References 31 publications

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“…Although the effect over the cell membrane receptors and the cell dynamics such as proliferation and molecular synthesis were not simulated in this work, we have previously assessed the in vitro effects of EFs and MFs over monolayer chondrocytes. In a previous study, the findings indicated that EFs of 4 and 8 mV/cm applied at 60 kHz sine‐wave form enhanced the proliferation rate and kept stable the synthesis of glycosaminoglycans . In our most recent work, it was evidenced that the best proliferation rate was obtained with MFs of 2 mT applied for 3 hours every 6 hours during 8 days.…”

Section: Discussionmentioning

confidence: 69%

“…Nevertheless, it has been evidenced that nano‐pulsed EFs of 60 kV/cm at 60 ns applied to different type of cells induced damage to the cytoskeleton, nuclear membrane, and telomere sequences . These results contrast with the findings reported by other studies that have produced promising outcomes in terms of proliferation, migration and molecular synthesis . Considering that cell‐recovery following external electrical or magnetic pulsing has been observed and is well documented, Pliquett et al, establish that destruction either in the nuclear membrane or other inner membranes could probably produce severe biological disruptions, leading to irreversibility and cell death …”

Section: Discussionmentioning

confidence: 97%

“…Some studies that use EFs and MFs as external biophysical stimulus have observed an increased in proliferation, migration, cellular differentiation and synthesis of proteins . Although the effect over the cell membrane receptors and the cell dynamics such as proliferation and molecular synthesis were not simulated in this work, we have previously assessed the in vitro effects of EFs and MFs over monolayer chondrocytes.…”

Section: Discussionmentioning

confidence: 99%

“…The EFs and MFs are being extensively investigated to find efficient therapeutic methods, for instance, to treat diseases without performing surgical interventions, since these stimuli increase cell metabolism fostering a faster healing of injured tissues. The EFs and MFs have demonstrated to have a positive effect on cell dynamics such as increasing the proliferative rate and stimulating the protein synthesis such as collagen, aggrecan, and glycosaminoglycans . This positive effect on cellular dynamics is due to an application of external stimuli over the cell membrane, which generates a longer activation of membrane proteins and a flow of ions such as Ca 2+ , Na + , and K +.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, they suggest that 50.000 V/m are required to produce an effect in the plasma membrane in round cells. There is experimental evidence proving that EFs applied at frequencies between 20 Hz and 448 kHz promote cell proliferation and protein synthesis . However, the most used frequencies are from 10 to 100 Hz because these are considered natural frequencies, and they have been reported as the most effective parameters for bone formation when tissues are stimulated .…”

Section: Introductionmentioning

confidence: 99%

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Effect of magnetic and electric fields on plasma membrane of single cells: A computational approach

Escobar

Vaca‐González

Guevara

et al. 2020

Engineering Reports

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Cell membrane is a lipid bilayer that allows the flow of ions through their ionic pumping proteins. The ionic flow can be stimulated with external stimuli to activate specific signaling pathways intracellularly. Although studies have applied electric and magnetic stimuli to modify the cell function, the parameters to stimulate the cell membrane are unknown. Accordingly, a computational model to simulate the effect of electric and magnetic fields on the cell membrane was developed. Cells were stimulated with electric fields from 45 × 103 V/m to 12.6 × 105 V/m and magnetic fields of 2 mT, at frequencies of 60 kHz, 10 MHz, and 1 GHz. Results showed that the electric fields applied to the cell membrane tend to increase according to the frequency used, while magnetic fields do not have any effect on it. It was observed that electric fields generate a high voltage concentrator in the cell membrane of ellipsoidal cells when a frequency window from 1 kHz to 1 GHz was applied. These findings demonstrate that depending on the intensity of the field and frequency, it was possible to stimulate different cell membrane zones. This model is a promising tool to establish the adequate parameters to stimulate cells, and accurately predict if the stimulation modifies the cell membrane potential.

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Section: Discussionmentioning

confidence: 69%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Effect of magnetic and electric fields on plasma membrane of single cells: A computational approach

Escobar

Vaca‐González

Guevara

et al. 2020

Engineering Reports

View full text Add to dashboard Cite

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In Vitro Evaluation of the Effect of Stimulation with Magnetic Fields on Chondrocytes

Escobar

Vaca‐González

Guevara

et al. 2019

Bioelectromagnetics

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Magnetic fields (MFs) have been used as an external stimulus to increase cell proliferation in chondrocytes and extracellular matrix (ECM) synthesis of articular cartilage. However, previously published studies have not shown that MFs are homogeneous through cell culture systems. In addition, variables such as stimulation times and MF intensities have not been standardized to obtain the best cellular proliferative rate or an increase in molecular synthesis of ECM. In this work, a stimulation device, which produces homogeneous MFs to stimulate cell culture surfaces was designed and manufactured using a computational model. Furthermore, an in vitro culture of primary rat chondrocytes was established and stimulated with two MF schemes to measure both proliferation and ECM synthesis. The best proliferation rate was obtained with an MF of 2 mT applied for 3 h, every 6 h for 8 days. In addition, the increase in the synthesis of glycosaminoglycans was statistically significant when cells were stimulated with an MF of 2 mT applied for 5 h, every 6 h for 8 days. These findings suggest that a stimulation with MFs is a promising tool that could be used to improve in vitro treatments such as autologous chondrocyte implantation, either to increase cell proliferation or stimulate molecular synthesis. Bioelectromagnetics. 2020;41:41–51 © 2019 Bioelectromagnetics Society

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Biological Response of Osteoblastic and Chondrogenic Cells to Graphene-Containing PCL/Bioactive Glass Bilayered Scaffolds for Osteochondral Tissue Engineering Applications

Deliormanlı

Atmaca

2018

Appl Biochem Biotechnol

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Graphene-containing 13-93 bioactive glass and poly(ε-caprolactone)-based bilayer, electrically conductive scaffolds were prepared for osteochondral tissue repair. Biological response of osteoblastic MC3T3-E1 and chondrogenic ATDC5 cells to the composite scaffolds was assessed under mono-culture and co-culture conditions. Cytotoxicity was investigated using MTT assay, cartilage matrix production was evaluated by Alcian blue staining, and mineralization of both types of cells in the different culture systems was observed by Alizarin red S staining. Results showed that osteoblastic and chondrogenic cells utilized in the study did not show toxic response to the prepared scaffolds under mono-culture conditions and higher cell viability rates were obtained in co-culture conditions. Larger mineralized areas were determined under co-culture conditions and calcium deposition amount significantly increased compared with that in control group samples after 21 days. Additionally, the amount of glycosaminoglycans synthesized in co-culture was higher compared to mono-culture conditions. Electric stimulation applied under mono-culture conditions suppressed the viability of MC3T3-E1 cells whereas it enhanced the viability rates of ATDC5 cells. The study suggests that the designed bilayered osteochondral constructs have the potential for osteochondral defect repair.

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An In Vitro Chondrocyte Electrical Stimulation Framework: A Methodology to Calculate Electric Fields and Modulate Proliferation, Cell Death and Glycosaminoglycan Synthesis

Cited by 30 publications

References 31 publications

Effect of magnetic and electric fields on plasma membrane of single cells: A computational approach

Effect of magnetic and electric fields on plasma membrane of single cells: A computational approach

In Vitro Evaluation of the Effect of Stimulation with Magnetic Fields on Chondrocytes

Biological Response of Osteoblastic and Chondrogenic Cells to Graphene-Containing PCL/Bioactive Glass Bilayered Scaffolds for Osteochondral Tissue Engineering Applications

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