An in vitro comparative antibacterial study of different concentrations of green tea extracts and 2% chlorhexidine on Enterococcus faecalis

Martina, Leena; Ebenezar, A V Rajesh; Ghani, M. F. Abdul; Narayanan, Ashwin; Sundaram, Meenakshi; Mohan, Ajit George

doi:10.4103/1658-5984.121504

Cited by 13 publications

(22 citation statements)

References 15 publications

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“…Microbial results showed that, in the 3.5% green tea extract irrigant group, there was statistically significant reduction in aerobic and anaerobic bacterial counts after irrigation, the results were in agreement with Martina et al, 2013the tube dilution method was used to determine the minimum inhibitory concentration (MIC; who showed that green tea extract had antibacterial activity against E. faecalis (10) .…”

Section: Discussionsupporting

confidence: 85%

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Evaluation of Antimicrobial Efficacy of Green Tea Extract Irrigant Versus Physiological Saline Following Root Canal Treatment of Infected Primary Molars in a Group of Egyptian Children : A Randomized Clinical Trial

Salem

Taha

Gwad

2017

Egyptian Dental Journal

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Section: Discussionsupporting

confidence: 85%

“…Mixing 35g of green tea extract with 100 ml of sterile boiling distilled water for 5 minutes were done and then filtered by filter paper. The dilutions were kept overnight at 37°C (10) .…”

Section: Preparation Of the Green Tea Extract Dilutionsmentioning

confidence: 99%

Evaluation of Antimicrobial Efficacy of Green Tea Extract Irrigant Versus Physiological Saline Following Root Canal Treatment of Infected Primary Molars in a Group of Egyptian Children : A Randomized Clinical Trial

Salem

Taha

Gwad

2017

Egyptian Dental Journal

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“…23 It shows antimicrobial activity from concentrations as low as 0.1%; and shows bactericidal activity at 2% and is biocompatible. 2425 …”

Section: Discussionmentioning

confidence: 99%

“…This technique is a well-accepted way of comparing the antibacterial effect of different dental materials, medicaments, etc. 24 Agar well diffusion was used for the combination of antimicrobial drugs with vehicles as though E-test being the latest method, it is not feasible for combination of drugs.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Activity of Endodontic Medicaments and Vehicles using Agar Well Diffusion Method on Facultative and Obligate Anaerobes

Nalawade

Bhat

Sogi

2016

International Journal of Clinical Pediatric Dentistry

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AimsThe aim of this study was to determine the relative antimicrobial effectiveness of these endodontic medicaments and various vehicles using an agar well diffusion assay.Materials and methodsDouble Antibiotic Paste(DAP), modified DAP, 2% Chlorhexidine gluconate and their combination with four vehicles namely Polyethylene glycol 400 (PEG), Propylene glycol (PG), combinations of PG with PEG and lastly Glycerine were tested using agar well diffusion assay. The minimum bactericidal concentration was noted against four standard strains of organisms ie Streptococcus mutans ATCC( American Type Culture Collection) 25175, Staphylococcus aureus ATCC 12598, Enterococcus faecalis ATCC 35550 and Eschericia coli ATCC 25922. Successful endodontic therapy depends upon thorough disinfection of root canals. In some refractory cases, routine endodontic therapy is not sufficient, so intracanal medicaments are used for proper disinfection of canals. Issues of resistance, limited spectrum of activity and lack of antifungal properties, the hunt for the ideal intracanal medicament continues. In this regard, the vehicles used to form the pastes play a supportive role by forming the appropriate consistency for placement and may dramatically influence their chemical characteristics like their solubility and diffusion. Thus, inorder to use safer and equally effective intracanal medicaments, Chlorhexidine gluconate is being unveiled in this study.ResultsThe difference between the four vehicles when combined with the same endodontic medicament studied above is nonsignificant (NS) except against Porphyromonas gingivalis. Propylene glycol is significantly effective than Glycerine when used with DAP ie C+M medicament combination. (p = 0.029)Conclusion2% chlorhexidine gluconate and modified DAP can definitely replace DAP and triple antibiotic paste as end-odontic medicaments with chlorhexidine having an added advantage of bactericidal action, substantivity, biocompatibility, low toxicity, and lesser chances of developing resistance.How to cite this articleNalawade TM, Bhat KG, Sogi S. Antimicrobial Activity of Endodontic Medicaments and Vehicles using Agar Well Diffusion Method on Facultative and Obligate Anaerobes. Int J Clin Pediatr Dent 2016;9(4):335-341.

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“…Many studies have examined the most effective way for removing E. faecalis from the root canal, and chlorhexidine has shown particularly high efficacy [2][3][4][5]. However, chlorhexidine is a synthetic chemical with known toxicity against certain cells, such as fibroblasts, polymorphonuclear leukocytes (PMNs), and other leukocytes, macrophages, and erythrocytes.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Efficacy of 6% Green Tea Extract and 2% Chlorhexidine Against Enterococcus Faecalis Biofilm in Vitro

Reflan

Meidyawati²,

Indrawati

2019

Int J App Pharm

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Objective: This study aimed to compare the antibacterial efficacy of green tea extract to chlorhexidine 2% against Enterococcus faecalis biofilm usingreal-time polymerase chain reaction (RT-PCR).Methods: E. faecalis strain ATCC 29212 colonies were collected from overnight cultures grown on brain–heart infusion agar (BHIA) and resuspendedat 108 CFU/ml according to a 0.5 McFarland standard. Aliquots of bacterial suspension (50 μl) were then inoculated onto sterile nitrocellulose filterdiscs place on BHIA and incubated aerobically at 37°C for 72 h. After incubation, the discs were removed, transferred into 10-ml phosphate-bufferedsaline (PBS) to loosen planktonic bacteria, and then incubated for 10 min at 37°C in 10 ml of 6% green tea extract, 2% chlorhexidine, or sterile PBS(control). Surviving E. faecalis cells were then quantified by RT-PCR.Results: Green tea extract reduced bacterial survival compared to control but was not as effective as chlorhexidine 2%.Conclusion: Green tea extract may help reduce oral E. faecalis biofilm. Moreover, specific antimicrobial compounds in green tea extract such asepigallocatechin-3-gallate should be tested as non-toxic alternatives to chlorhexidine.

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An in vitro comparative antibacterial study of different concentrations of green tea extracts and 2% chlorhexidine on Enterococcus faecalis

Cited by 13 publications

References 15 publications

Evaluation of Antimicrobial Efficacy of Green Tea Extract Irrigant Versus Physiological Saline Following Root Canal Treatment of Infected Primary Molars in a Group of Egyptian Children : A Randomized Clinical Trial

Evaluation of Antimicrobial Efficacy of Green Tea Extract Irrigant Versus Physiological Saline Following Root Canal Treatment of Infected Primary Molars in a Group of Egyptian Children : A Randomized Clinical Trial

Antimicrobial Activity of Endodontic Medicaments and Vehicles using Agar Well Diffusion Method on Facultative and Obligate Anaerobes

Antibacterial Efficacy of 6% Green Tea Extract and 2% Chlorhexidine Against Enterococcus Faecalis Biofilm in Vitro

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