An In Vitro Human Epithelial Cell Culture System for Studying the Pathogenesis of Chlamydia trachomatis

Pb, Wyrick; Ch, Davis; St, Knight; Choong, John; Je, Raulston; Schramm, Nara

doi:10.1097/00007435-199309000-00002

Cited by 19 publications

(23 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Subsequently, it was shown that epithelial cells were more sensitive to C. trachomatis serovar E in the estrogen-dominant (proliferative) stage of the human menstrual cycle than at other stages (17,30). When estrogendominant-phase cells were grown and maintained in the normal human physiological concentration of estrogen (10 Ϫ10 M), chlamydial attachment and infectivity was enhanced Ն80%.…”

Section: Discussionmentioning

confidence: 99%

“…HeLa cells (epithelial cells from a cervical adenocarcinoma) have been used for most experimental analyses and are highly sensitive to chlamydial infection, but they are less representative of primary, differentiated target epithelial cells (4). As an alternative to the established transformed cell lines, a few studies have been performed with primary, hormone-responsive polarized human endometrial epithelial cells derived from women undergoing a hysterectomy for benign disease (17,19,29,30). Important information about chlamydial attachment and infection which differed, in several aspects, from data obtained from non-hormone-responsive, nonpolarized, chlamydia-infected HeLa cells was obtained: (i) chlamydial inclusion development in primary human cervical and endometrial epithelial cells was patchy and uneven in distribution; (ii) levels of chlamydial attachment to and infectivity of primary human endometrial epithelial cells were significantly greater in estrogen-dominant than in progesteronedominant cells; (iii) entry of infectious elementary bodies (EBs) into polarized, hormone-responsive, primary human endometrial epithelial cells was mediated predominately by clathrin-coated pit receptor-mediated endocytosis; and (iv) exit of chlamydiae from primary polarized human epithelial cells was pathogenesis directed, i.e., human noninvasive C. trachomatis serovar E progeny exited via the apical surface, whereas invasive C. trachomatis lymphogranuloma venereum-causing progeny were released at the basal domain.…”

mentioning

confidence: 99%

“…This study evaluates the potential for using the C. suis S-45 swine primary genital tract epithelial cell culture model to follow up on previous data obtained from primary human endometrial epithelial cells (17,19,30), especially to study the influence of hormones on chlamydial attachment, adhesionreceptor interactions, and entry and development in genital tract cells, as well as microbicidal efficacy.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Primary Cultures of Female Swine Genital Epithelial Cells In Vitro: a New Approach for the Study of Hormonal Modulation of Chlamydia Infection

et al. 2003

Self Cite

View full text Add to dashboard Cite

Previous studies have demonstrated that female reproductive hormones influence chlamydial infection both in vivo and in vitro. Due to the reduced availability of human genital tissues for research purposes, an alternative hormone-responsive model system was sought to study chlamydial pathogenesis. Mature female swine eliminated from breeding programs were selected as the animals of choice because of the similarity of a sexually transmitted disease syndrome and sequelae in swine to a disease syndrome and sequelae found in humans, because of the near identity of a natural infectious chlamydial isolate from swine to Chlamydia trachomatis serovar D from humans, and because a pig's epithelial cell physiology and the mean length of its estrous cycle are similar to those in humans. Epithelial cells from the cervix, uterus, and horns of the uterus were isolated, cultivated in vitro in Dulbecco's minimum essential medium-Hanks' F-12 (DMEM-F-12) medium with and without exogenous hormone supplementation, and analyzed for Chlamydia suis S-45 infectivity. The distribution of chlamydial inclusions in swine epithelial cells was uneven and was influenced by the genital tract site and hormone status. This study confirmed that, like primary human endometrial epithelial cells, estrogen-dominant swine epithelial cells are more susceptible to chlamydial infection than are progesterone-dominant cells. Further, the more differentiated luminal epithelial cells were more susceptible to infection than were glandular epithelial cells. Interestingly, chlamydial growth in mature luminal epithelia was morphologically more active than in glandular epithelia, where persistent chlamydial forms predominated. Attempts to reprogram epithelial cell physiology and thereby susceptibility to chlamydial infection by reversestage, exogenous hormonal supplementation were unsuccessful. Freshly isolated primary pig epithelial cells frozen at ؊80°C in DMEM-F-12 medium with 10% dimethyl sulfoxide for several weeks can, after thawing, reform characteristic polarized monolayers in 3 to 5 days. Thus, primary swine genital epithelia cultured ex vivo appear to be an excellent cell model for dissecting the hormonal modulation of several aspects of chlamydial pathogenesis and infection.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Primary Cultures of Female Swine Genital Epithelial Cells In Vitro: a New Approach for the Study of Hormonal Modulation of Chlamydia Infection

et al. 2003

Self Cite

View full text Add to dashboard Cite

show abstract

“…Epithelial cell cultures are most commonly used for in vitro growth and characterization of C. trachomatis infection (69). C. trachomatis infection of HeLa cells, SiHa human cervical carcinoma epithelial cells, and HEp2 human epithelial cells was used to demonstrate that IL-1␣ regulates IL-8 production in these cell lines during infection (70).…”

Section: Immune Pathway Elucidated By In Vitro Culture Modelsmentioning

confidence: 99%

Human and Pathogen Factors Associated with Chlamydia trachomatis-Related Infertility in Women

et al. 2015

View full text Add to dashboard Cite

SUMMARY Chlamydia trachomatis is the most common bacterial sexually transmitted pathogen worldwide. Infection can result in serious reproductive pathologies, including pelvic inflammatory disease, ectopic pregnancy, and infertility, in women. However, the processes that result in these reproductive pathologies have not been well defined. Here we review the evidence for the human disease burden of these chlamydial reproductive pathologies. We then review human-based evidence that links Chlamydia with reproductive pathologies in women. We present data supporting the idea that host, immunological, epidemiological, and pathogen factors may all contribute to the development of infertility. Specifically, we review the existing evidence that host and pathogen genotypes, host hormone status, age of sexual debut, sexual behavior, coinfections, and repeat infections are all likely to be contributory factors in development of infertility. Pathogen factors such as infectious burden, treatment failure, and tissue tropisms or ascension capacity are also potential contributory factors. We present four possible processes of pathology development and how these processes are supported by the published data. We highlight the limitations of the evidence and propose future studies that could improve our understanding of how chlamydial infertility in women occurs and possible future interventions to reduce this disease burden.

show abstract

“…The HEC-1B cells, grown in a polarized manner [35] on commercial inserts (BioCoat Matrigel invasive chambers, 0.3 cm 2 , Becton Dickinson Labware), were cultivated in Dulbecco's modified Eagle MEM (Life Technologies GIBCO-BRL, Grand Island, NY) supplemented with 10% fetal calf serum (Hyclone, Logan, UT) and 10 mM HEPES, pH 7.3 (DMEM) and maintained at 35 C in an atmosphere of 5% carbon dioxide and 95% humidified air.…”

Section: Growth Of Chlamydia and Eukaryotic Host Cellmentioning

confidence: 99%

Ultrastructural analysis of chlamydial antigen-containing vesicles everting from the Chlamydia trachomatis inclusion

Giles

Whittimore

LaRue

et al. 2006

Microbes and Infection

Self Cite

View full text Add to dashboard Cite

An In Vitro Human Epithelial Cell Culture System for Studying the Pathogenesis of Chlamydia trachomatis

Cited by 19 publications

References 33 publications

Primary Cultures of Female Swine Genital Epithelial Cells In Vitro: a New Approach for the Study of Hormonal Modulation of Chlamydia Infection

Primary Cultures of Female Swine Genital Epithelial Cells In Vitro: a New Approach for the Study of Hormonal Modulation of Chlamydia Infection

Human and Pathogen Factors Associated with Chlamydia trachomatis-Related Infertility in Women

Ultrastructural analysis of chlamydial antigen-containing vesicles everting from the Chlamydia trachomatis inclusion

Contact Info

Product

Resources

About