An in vitro method to evaluate hemolysis of human red blood cells (RBCs) treated by airborne particulate matter (PM10)

Mesdaghinia, Alireza; Pourpak, Zahra; Naddafi, Kazem; Nabizadeh, Ramin; Alizadeh, Zahra; Rezaei, Soheila; Mohammadi, Amir H.; Faraji, Maryam

doi:10.1016/j.mex.2019.01.001

Cited by 56 publications

(31 citation statements)

References 4 publications

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“…After centrifugation, the supernatant incubated with hybrid hydrogels or fibrin gels after 24 h showed a light red color similar to that of the saline treatment (control), indicating the good hemocompatibility of the hybrid hydrogels (Figure 4D). Furthermore, the percentage hemolysis of the hybrid hydrogels was within the safe limit for hemostatic biomaterials reported in the literature [24] (Figure 4E).…”

Section: Excellent In Vitro Blood Clotting and Biocompatibility Of Th...supporting

confidence: 72%

Adhesive Hemostatic Hydrogel with Ultrafast Gelation Arrests Acute Upper Gastrointestinal Hemorrhage in Pigs

Xia

Wang

et al. 2021

Adv Funct Materials

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Upper gastrointestinal (GI) hemorrhage is a common clinical emergency worldwide. Endoscopic hemostasis is the current first line treatment. Nevertheless, for patients with severe active bleeding and challenging anatomy, endoscopic management can be still difficult and usually requires a higher level of surgical expertise. A simple and effective method of endoscopic hemostasis is therefore in acute clinical demand. Herein a hemostatic hybrid hydrogel comprised of hyaluronic acid and polyethylene glycol stabilized by thiourea-catechol coupling and disulfide bonds is reported. The hybrid hydrogels exhibit enhanced mechanical properties, short gelation time, and good blood clotting ability compared with fibrin gels. The in vivo hemostatic efficacy is confirmed in a rat model of arterial bleeding, in which the rapid gelation of hybrid hydrogels after topical application effectively arrests blood loss with no rebleeding occurring during resuscitation when blood pressure approximately recovers to normal. A large animal study further demonstrates the efficacy of hybrid hydrogels to achieve endoscopic hemostasis against upper GI hemorrhage in pigs. Follow-up observations show that the hybrid hydrogels can remain adherent at the bleeding wound for 48 h, indicating the sustained hemostatic function in vivo. Collectively, this work demonstrates a promising hemostatic hydrogel for endoscopic hemostasis in upper GI hemorrhage.

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Section: Excellent In Vitro Blood Clotting and Biocompatibility Of Th...supporting

confidence: 72%

Adhesive Hemostatic Hydrogel with Ultrafast Gelation Arrests Acute Upper Gastrointestinal Hemorrhage in Pigs

Xia

Wang

et al. 2021

Adv Funct Materials

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show abstract

“…As shown in Figure , in the 4‐hour exposure system, the experimental results showed that when the UFCB concentration was lower than 40 mg/L, the hemolysis ratio of hRBCs would not be induced. UFCB could not significantly change the permeability of hRBCs membrane, which means that the membrane was not completely destroyed . The damage to hRBCs was relatively low, providing a guarantee for the following experimental determination.…”

Section: Resultsmentioning

confidence: 91%

Hematological effects of ultrafine carbon black on red blood cells and hemoglobin

Pan

Zhang

et al. 2019

J Biochem & Molecular Tox

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The harmful effects of ultrafine particles (UFPs) in the atmosphere have caused widespread concern. Ultrafine carbon black (UFCB) is an important component of UFPs. In this study, we explored the impact of UFCB on the structure, the antioxidant defense system, and the ATPase activity of human red blood cells (hRBCs). It was found that UFCB decreased the activity of SOD (73.58%), CAT (89.79%), and GSH‐Px (81.02%), leading to oxidative stress in hRBCs. UFCB had no destructive effect on the structure of hRBCs in 4 hours. ATPase activity increased (119.34%) and UFCB had weakly stimulated the cell membrane. On the molecular level, spectroscopic experiments showed that bovine hemoglobin (BHb) can bind to the UFCB by electrostatic force, leading to the shrinking of the BHb skeleton and increase in microenvironment polarity. This study demonstrates the negative hematological effect of UFCB on hemoglobin and hRBCs and reveals the potential risks in animals and humans.

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“…Similarly, in our previous study, elevated neutrophils counting indicated that PM served as an external stimulant to the body, inducing inflammatory responses (Jiang et al, 2020). Hemolysis is the premature destruction of red blood cells (RBCs) membranes and it can be induced by the mineral components adsorbed on the particles (Mesdaghinia et al, 2019). In the current study, no significant changes were observed in the relevant red blood cell index, which indicates that PM did not cause the destruction of RBCs.…”

Section: Discussionmentioning

confidence: 73%

The Role of Nrf2 in the PM-Induced Vascular Injury Under Real Ambient Particulate Matter Exposure in C57/B6 Mice

Gao

Luo

et al. 2021

Front. Pharmacol.

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Short-and long-term exposure to particulate matter (PM) has been associated with cardiovascular disease (CVD). It is well recognized that oxidative stress is a potential major mechanism in PM-induced vascular injuries, in which the nuclear factor E2-related factor 2 (Nrf2) signaling pathway plays a critical role. In the current study, a Nrf2 knockout mouse model was used in combination with an individual ventilated cage (IVC)-based real-ambient PM exposure system to assess the potential vascular injury and the potential role of Nrf2 in the angiotensin II (Ang II)-associated vascular injury. After 6-or 11-week exposure to PM, the histopathology assay revealed that PM exposure resulted in the thickening of the walls of vascular. After 6 weeks exposure to PM, the ELISA assay revealed that PM exposure resulted in the elevated plasma concentration of Ang II. The expression levels of genes of interest were then further investigated with quantitative real-time PCR. Notably, the results showed that Angiotensinogen (AGT), Angiotensin converting enzyme (ACE) and Angiotensin type I receptor (AT1R) were involved in PM-induced pathological changes. Western blotting for ACE showed similar results. Moreover, the extent of vascular thickening and the Ang II elevation was most prominent in the Nrf2 gene knockout PM exposure group (KOE). Furthermore, the expression of Nrf2 downstream relevant genes (HO1, Nqo1, Gclc, Gsta4) were significantly enhanced in the wildtype PM exposure group (WTE), while those were remarkably suppressed in the Nrf2 gene knockout groups. The ELISA result of monocyte chemoattractant protein-1 (MCP-1) serum levels in the KOE group was significantly higher in relation to that in the Nrf2 knockout control group (KOC). In summary, PM exposure is associated with thickening of vascular wall, while Nrf2 knockout may further enhance this effect. A potential mechanistic contributor of such effects is the activation of ACE/ANGII/AT1R axis, in which Nrf2 played a regulatory role.

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An in vitro method to evaluate hemolysis of human red blood cells (RBCs) treated by airborne particulate matter (PM10)

Cited by 56 publications

References 4 publications

Adhesive Hemostatic Hydrogel with Ultrafast Gelation Arrests Acute Upper Gastrointestinal Hemorrhage in Pigs

Adhesive Hemostatic Hydrogel with Ultrafast Gelation Arrests Acute Upper Gastrointestinal Hemorrhage in Pigs

Hematological effects of ultrafine carbon black on red blood cells and hemoglobin

The Role of Nrf2 in the PM-Induced Vascular Injury Under Real Ambient Particulate Matter Exposure in C57/B6 Mice

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