An in vitro microfluidic gradient generator platform for antimicrobial testing

DiCicco, Matthew; Neethirajan, Suresh

doi:10.1007/s13206-014-8406-6

Cited by 20 publications

(20 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, the works of Mao et al (2003) and Englert et al (2009) employed this microfluidic tool to explore Escherichia coli chemotaxis under different chemo effector concentrations in parabolic and linear concentration gradient profiles, respectively. While DiCicco and Neethirajan (2014) used a linear gradient profile system to investigate antimicrobial efficacy against formation of Staphylococcus pseudintermedius biofilm, which is methicillin resistant (MRSP) (Figure ). These kinds of investigations could be employed also in industrial biotechnology, since enzymatic and microbial cultivation media conditions, such as substrate and produced metabolites concentrations, can be the key point to new findings to overcome low efficiency problems in bioprocess.…”

Section: Microfluidics: Approaches For Industrial Biotechnologymentioning

confidence: 99%

“…Microchamber was used for antimicrobial testing of fosfomycin against MRSP biofilms. DiCicco and Neethirajan (2014) . Reproduced with permission from Ref.…”

Section: Microfluidics: Approaches For Industrial Biotechnologymentioning

confidence: 99%

“…The dynamic mode of concentration gradients generation in microchannels enables monitoring the biochemical signals caused by different stimuli in a real condition. 7 115 used a linear gradient profile system to investigate antimicrobial efficacy against formation of Staphylococcus pseudintermedius biofilm, which is methicillin resistant (MRSP) (Figure 3). These kinds of investigations could be employed also in industrial biotechnology, since enzymatic and microbial cultivation media conditions, such as substrate and produced metabolites concentrations, can be the key point to new findings to overcome low efficiency problems in bioprocess.…”

Section: Microfluidic Concentration Gradient Generatorsmentioning

confidence: 99%

“…Microfluidics based on flow-gradient with network microchannel to gradient generation.Microchamber was used for antimicrobial testing of fosfomycin against MRSP biofilms. DiCicco and Neethirajan (2014) 115. Reproduced with permission from Ref 115…”

mentioning

confidence: 99%

See 3 more Smart Citations

Microfluidic tools toward industrial biotechnology

Oliveira

Pessoa

Bastos

et al. 2016

Biotechnology Progress

View full text Add to dashboard Cite

Microfluidics is a technology that operates with small amounts of fluids and makes possible the investigation of cells, enzymes, and biomolecules and encapsulation of biocatalysts in a greater variety of conditions than permitted using conventional methods. This review discusses technological possibilities that can be applied in the field of industrial biotechnology, presenting the principal definitions and fundamental aspects of microfluidic parameters to better understand advanced approaches. Specifically, concentration gradient generators, droplet-based microfluidics, and microbioreactors are explored as useful tools that can contribute to industrial biotechnology. These tools present potential applications, inclusive as commercial platforms to optimizing in bioprocesses development as screening cells, encapsulating biocatalysts, and determining critical kinetic parameters. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1372-1389, 2016.

show abstract

Section: Microfluidics: Approaches For Industrial Biotechnologymentioning

confidence: 99%

“…Microchamber was used for antimicrobial testing of fosfomycin against MRSP biofilms. DiCicco and Neethirajan (2014) . Reproduced with permission from Ref.…”

Section: Microfluidics: Approaches For Industrial Biotechnologymentioning

confidence: 99%

Section: Microfluidic Concentration Gradient Generatorsmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Microfluidic tools toward industrial biotechnology

Oliveira

Pessoa

Bastos

et al. 2016

Biotechnology Progress

View full text Add to dashboard Cite

show abstract

“…Microfluidics overcomes the limitations of traditional methods, and offers techniques for cultivating and investigating microbes in a more clinically realistic and physiologically relevant environment. Microfluidic devices have been become powerful tools used in cell biology applications, including cell culture [21] and handling and analysis [22,23]. These devices allow researchers to better mimic in vivo physiological conditions due to their unique properties of the microenvironment control, customizable microstructure, and perfusion controllability [24].…”

Section: Introductionmentioning

confidence: 99%

Single Cell Chemotactic Responses of Helicobacter pylori to Urea in a Microfluidic Chip

2016

Self Cite

View full text Add to dashboard Cite

Abstract:Helicobacter pylori is a spiral-shaped bacterium that grows in the human digestive tract; it infects~50% of the global population. H. pylori induce inflammation, gastroenteritis, and ulcers, which is associated with significant morbidity and may be linked to stomach cancer in certain individuals. Motility is an essential virulence factor for H. pylori, allowing it to migrate toward and invade the epithelial lining of the stomach to shelter it from the harsh environment of the stomach. H. pylori senses pH gradients and use polar flagella to move towards the epithelium where the pH approaches neutrality. However, its chemotaxis behaviors are incompletely understood. Previous in vitro tests examining the response of H. pylori to chemical gradients have been subjected to substantial limitations. To more accurately mimic/modulate the cellular microenvironment, a nanoporous microfluidic device was used to monitor the real time chemotactic activity of single cell of H. pylori in response to urea. The results showed that microfluidic method is a promising alternative for accurate studying of chemotactic behavior of H. pylori, the application of which may also be extended in the studies of other bacteria.

show abstract

Microfluidic Evolution‐On‐A‐Chip Reveals New Mutations that Cause Antibiotic Resistance

Zoheir

Späth

Niemeyer

et al. 2021

Small

View full text Add to dashboard Cite

Microfluidic devices can mimic naturally occurring microenvironments and create microbial population heterogeneities ranging from planktonic cells to biofilm states. The exposure of such populations to spatially organized stress gradients can promote their adaptation into complex phenotypes, which are otherwise difficult to achieve with conventional experimental setups. Here a microfluidic chip that employs precise chemical gradients in consecutive microcompartments to perform microbial adaptive laboratory evolution (ALE), a key tool to study evolution in fundamental and applied contexts is described. In the chip developed here, microbial cells can be exposed to a defined profile of stressors such as antibiotics. By modulating this profile, stress adaptation in the chip through resistance or persistence can be specifically controlled. Importantly, chip‐based ALE leads to the discovery of previously unknown mutations in Escherichia coli that confer resistance to nalidixic acid. The microfluidic device presented here can enhance the occurrence of mutations employing defined micro‐environmental conditions to generate data to better understand the parameters that influence the mechanisms of antibiotic resistance.

show abstract

An in vitro microfluidic gradient generator platform for antimicrobial testing

Cited by 20 publications

References 26 publications

Microfluidic tools toward industrial biotechnology

Microfluidic tools toward industrial biotechnology

Single Cell Chemotactic Responses of Helicobacter pylori to Urea in a Microfluidic Chip

Microfluidic Evolution‐On‐A‐Chip Reveals New Mutations that Cause Antibiotic Resistance

Contact Info

Product

Resources

About