An in vitro nerve agent brain poisoning transwell model for convenient and accurate antidote evaluation

A mix & act liposomes of phospholipase A2-phosphatidylserine for acute brain detoxification by blood‒brain barrier selective-opening

“…As shown in Fig. 2 A, these liposomes with POPS could only transport FLU across the barrier with the assistance of PLA2 or NECA; this was proven by monitoring the FLU levels in the lower chamber of a Transwell system in vitro 10 , 23 , 24 . For the solid nanoparticles with MSNs, none of the opening materials used could open the BBB to allow these nanoparticles into the lower chamber ( Supporting Information Fig.…”

“…Rat brain microvascular endothelial cells (BMECs) were separated and cultivated to establish BBB model in vitro as previous reports 10 , 23 , 24 . BMECs were plated on fibronectin-coated 0.4 μm pore size Transwell Plates (24 mm Transwell®, Beijing Labgic Technology Co., Ltd., Beijing, China), at a density of 1 × 10 5 cells per well, and continuous growth for 2 weeks to allow the BMECs to form a monolayer.…”