An In Vitro Study: Does Adding Iodine Potassium Iodide and Cetrimide to Calcium Hydroxide Paste Enhance Its Antimicrobial Effect Against Oral Biofilms?

Abstract

This study aimed to evaluate the antibiofilm effect of calcium hydroxide (CH), 0.5% iodine potassium iodide (IKI), and 0.5% cetrimide (CTR), alone and in combinations on one-week and three-week-old biofilms. Materials and methodsGingival plaque was collected, and biofilms were grown in vitro anaerobically. Biofilms were exposed to each of the three medicaments and their combinations for one day, one week, and two weeks. Proportions of dead and live bacteria in the biofilms were evaluated. ResultsThe killing of bacteria by different medicaments in the three-week-old biofilm was lower than in the oneweek-old biofilm (p<0.05). The efficacy of IKI and CTR in killing bacteria was weaker than that of CH, but the highest efficacy in killing was achieved when all three were combined (p<0.05). There was no significant difference in the antibiofilm effect between a day's exposure to the mixture of the three medicaments and one or two weeks of treatment with CH alone (p>0.05). ConclusionsThree-week-old biofilms are more resistant to medicaments than one-week-old biofilms. Combining IKI and CTR with CH resulted in a stronger antibiofilm effect than using CH alone. Mixing the three medicaments may enable obtaining the desired clinical effect in a shorter exposure time.

Locating and Treating Three Calcified Canals in a Mandibular First Molar With Radix Entomolaris and Five Canals: A Case Report

“…The primary treatment for AP is root canal therapy, which focuses on eradicating bacterial infections, preventing reinfection, and fostering the healing of affected periapical tissues. The effectiveness of root canal treatment hinges on meticulously cleaning and shaping the root canals to remove diseased tissue, bacteria, and their byproducts [5][6][7][8][9][10][11], followed by filling the canals with an inert material to preclude future microbial ingress [12][13][14][15][16].…”