An in vitro trial to study the effect of inclusion of different levels of coconut oil on sheep rumen methanogenesis and fermentation processes

Mukharji, Tisa; Srivastava, Aakash; Srivastava, Meera

doi:10.20510/ukjpb/2/i6/91174

Cited by 2 publications

(1 citation statement)

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“…Results from the present in vitro study indicated that the silkworm pupae oil supplementation decreased total gas, methane production, and rumen protozoal population. Similar to the present results, a linear decrease in total gas production was reported with graded supplementation of coconut oil [23]. Pilajun and Wanapat [24] reported that supplementation of coconut and almond oil either alone (5%) or combination (50:50) at 5% with variable roughage and concentrate ratio revealed variable response on gas production.…”

Section: Discussionsupporting

confidence: 90%

In vitro evaluation of graded level of Silkworm pupae (Bombyx mori) oil on methane production, fermentation characteristics, and protozoal populations

et al. 2020

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Aim: The present study was undertaken to evaluate the effect of variable levels of silkworm pupae oil and roughage: concentrate ratio on in vitro methane production, fermentation characteristics, and rumen protozoa population. Materials and Methods: In vitro gas production study (24 h) was performed with graded levels of silkworm pupae oil, namely, 0.5, 1, 2, 4, and 5% of the basal diet and four variable dietary regimes consisting roughage and concentrate in different proportions (70:30, 60:40, 50:50, and 40:60). At the end of incubation, gas samples were analyzed for methane, while fermented rumen liquor was used for protozoa enumeration. A separate set of incubations was carried out for the determination of in vitro dry matter digestibility. Results: Results from the in vitro studies revealed no adverse impact of the silkworm pupae oil supplementation up to 2% level on total gas production. However, supplementation beyond 2% has shown a reduction in total gas production. Incubation with variable levels (0.5-5%) of silkworm pupae oil with different dietary regimes indicated negligible (3-5%) to a substantial reduction (25-30%) on methane production. A graded decrement in methane production was recorded with increasing levels of silkworm pupae oil. Similarly, the protozoal populations were decreased from 10 to 51.5% with graded levels of silkworm pupae oil in different dietary regimes as studies did not reveal any significant (p>0.05) variation between 2 and 4% of oil supplementation. Conclusion: The silkworm pupae oil supplementation at 2% level decreases methane production by 12-15% without any adverse impact on feed fermentation. Oil supplementation may have a more pronounced effect on methane reduction if added to high roughage diet at in vitro conditions. However, in vivo, studies in ruminants are warranted to confirm the methane reduction with silkworm pupae oil supplementation.

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Section: Discussionsupporting

confidence: 90%

In vitro evaluation of graded level of Silkworm pupae (Bombyx mori) oil on methane production, fermentation characteristics, and protozoal populations

et al. 2020

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Inclusion of Different Levels of Eucalyptus Oil: An In Vitro Trial to Study the Effect on Sheep Rumen Methanogenesis and Fermentation Processes

Mukharji¹,

Srivastava²

2015

PBJ

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An in vitro trial was conducted in fermentation vessels under anaerobic conditions using composite feed-1 and composite feed-2 with inclusion of different levels of Eucalyptus oil (EO) along with buffered rumen liquor of sheep to observe the effects of oil on rumen methanogenesis and fermentation. The CF-1 consisted of 40% of concentrate and CF-2 had 25 % of concentrate.The various concentrations of EO used in present study were 0.5,1 and 2 µl/ml. During the present investigation it was observed that on increasing concentration of EO in CF-1 and CF-2 resulted in linear decrease in total gas production up to 74.26% in CF-1; and 31.01% by inclusion of EO in CF-2, respectively. The findings suggest that decrease in total gas production and methane concentration was highly pronounced in CF-1 compared to CF-2. The rumen digestibility in CF-1 and CF-2 also decreased significantly (p < 0.01) from control. A significant decrease in NH 3 -N compared to control was also documented. As EO in both the feeds was found to inhibit deamination activity, the ammonia production decreased with increase in the efficiency of protein utilization. The inclusion of the oil decreased the Total Volatile Fatty Acids (TVFA) concentration significantly (p < 0.01). The A:P ratio increased by inclusion in CF-2 but not in CF-1. The pH was effected on inclusion of EO in CF-2 whereas inclusion of EO in CF-1 exhibited non significant effect. Therefore, the difference in Volatile Fatty acids (VFA) pattern due to essential oil might be because of different diets and pH in different experiments. The inclusion of EO decreased the protozoa number significantly (p < 0.01). EO was found to decrease fermentation in a manner that even at 0.5 µl/ml concentration there was a significant decrease in GP, ME, SCFA, TDDM and MBP. It could therefore be envisaged that increasing the proportion of concentrate in diets and using EO as a feed additive increases the rumen efficiency by reducing the methane concentration and gas production.

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An in vitro trial to study the effect of inclusion of different levels of coconut oil on sheep rumen methanogenesis and fermentation processes

Cited by 2 publications

References 45 publications

In vitro evaluation of graded level of Silkworm pupae (Bombyx mori) oil on methane production, fermentation characteristics, and protozoal populations

In vitro evaluation of graded level of Silkworm pupae (Bombyx mori) oil on methane production, fermentation characteristics, and protozoal populations

Inclusion of Different Levels of Eucalyptus Oil: An In Vitro Trial to Study the Effect on Sheep Rumen Methanogenesis and Fermentation Processes

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