An in vivo rat model simulating imaging of human kidney by diagnostic ultrasound with gas-body contrast agent

Miller, Douglas L.; Dou, Chunyan; Wiggins, Roger C.; Wharram, Bryan L.; Goyal, Meera; Williams, A.R.

doi:10.1016/j.ultrasmedbio.2006.07.033

Cited by 31 publications

(80 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, the data from exposure-response tests using identical DUS exposure methods in three previous studies were incorporated into this study for comparison to the newer data. Data for 1.5 MHz DUS were included from 25 rats scanned with a tissue mimicking path (Miller et al 2007a) and 6 rats scanned with a waterpath (Miller et al 2007c). Data for 2.5 MHz B mode flash echo imaging were included from 22 rats (Miller et al 2007b).…”

Section: Methodsmentioning

confidence: 99%

“…The diagnostic machine was a GE Vingmed System V (General Electric Co., Cincinnati OH) with cardiac phased array probes. Some previously published results from the FPA 2.5 probe were included in this study for 1.5 MHz intermittent imaging (Miller et al 2007a, c). In addition, previously published results obtained using a Powervision 8000 (model SSA-390A, Toshiba Corporation, Tochigi-Ken, JPN) with PVN-375AT probe were included in this study for data on 2.5 MHz intermittent imaging (B mode Flash Echo Imaging mode) (Miller et al 2007b).…”

Section: Ultrasound Exposure Systemsmentioning

confidence: 99%

“…Characteristics for each of the eleven exposure arrangements are listed in Table 1. As described previously, the probes were clamped in a water bath with the right kidney of a rat located at the focal zone (Miller et al 2007a). The selected frequency in this study for the FPA 2.5 probe was 3.6 MHz (3.2 MHz actual frequency measured from the pulse waveform) with a 26 Hz frame rate.…”

Section: Ultrasound Exposure Systemsmentioning

confidence: 99%

“…There was some uncertainty in the actual in situ exposure in this study, because the probe was in contact with the rats (i. e., with the kidney center only about 1 cm away). In independent research, the GCH bioeffect was confirmed using Definity® with a 1.5 MHz probe scanning rats mounted in a water bath (to assure placement of the kidney at the focal maximum of the field) and the threshold was comparable at MI=0.6 (Miller et al 2007a). In another study using a different DUS machine operating at 2.5 MHz, the apparent threshold for GCH was found to be MI=0.95 using similar in vivo conditions (Miller et al 2007b).…”

Section: Introduction and Literaturementioning

confidence: 99%

“…Renal imaging is an important application of contrast aided diagnostic ultrasound (Wei et al 2001;Robbin et al 2003;Correas et al 2006), and therefore the GCH bioeffect is directly relevant to the cavitation bioeffects problem in DUS safety assurance. Diagnostic ultrasound scanners were utilized to the extent possible, including the previously published work at 1.5 MHz (Miller et al 2007a) and 2.5 MHz (Miller et al 2007b). The laboratory exposure system was used to simulate diagnostic ultrasound exposure for higher PRPAs than those available with our diagnostic equipment.…”

Section: Introduction and Literaturementioning

confidence: 99%

See 4 more Smart Citations

Frequency Dependence of Kidney Injury Induced by Contrast-Aided Diagnostic Ultrasound in Rats

Miller¹,

Dou²,

Wiggins³

2008

Ultrasound in Medicine & Biology

Self Cite

View full text Add to dashboard Cite

This study was performed to examine the frequency dependence of glomerular capillary hemorrhage (GCH) induced by contrast aided diagnostic ultrasound in rats. Diagnostic ultrasound (DUS) scanners were utilized for exposure at 3.2, 5.0 and 7.4 MHz, and previously published data at 1.5 and 2.5 MHz also was included. A laboratory exposure system was used to simulate DUS exposure at 1.0, 1.5, 2.25, 3.5, 5.0 and 7.5 MHz with higher peak rarefactional pressure amplitudes (PRPAs) than were available from our DUS systems. The right kidneys of rats mounted in a water bath were exposed to intermittent image pulse sequences at 1 s intervals during infusion of diluted ultrasound contrast agent. The percentage of GCH was zero for low PRPAs, and then rapidly increased with increasing PRPAs above an apparent threshold, p t . The values of p t were approximately proportional to the ultrasound frequency, f, such that p t /f was approximately 0.5 MPa/MHz for DUS and 0.6 MPa/MHz for laboratory-system exposures. The increasing thresholds with increasing frequency limited the GCH effect for contrast aided DUS, and no GCH was seen for DUS at 5.0 or 7.4 MHz for the highest available PRPAs.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Ultrasound Exposure Systemsmentioning

confidence: 99%

Section: Ultrasound Exposure Systemsmentioning

confidence: 99%

Section: Introduction and Literaturementioning

confidence: 99%

Section: Introduction and Literaturementioning

confidence: 99%

See 3 more Smart Citations

Frequency Dependence of Kidney Injury Induced by Contrast-Aided Diagnostic Ultrasound in Rats

Miller¹,

Dou²,

Wiggins³

2008

Ultrasound in Medicine & Biology

Self Cite

View full text Add to dashboard Cite

show abstract

Contrast Agent‐Free 3D Renal Ultrafast Doppler Imaging Reveals Vascular Dysfunction in Acute and Diabetic Kidney Diseases

Oh,

Lee,

Heo

et al. 2023

Advanced Science

View full text Add to dashboard Cite

To combat the irreversible decline in renal function associated with kidney disease, it is essential to establish non‐invasive biomarkers for assessing renal microcirculation. However, the limited resolution and/or vascular sensitivity of existing diagnostic imaging techniques hinders the visualization of complex cortical vessels. Here, a 3D renal ultrafast Doppler (UFD) imaging system that uses a high ultrasound frequency (18 MHz) and ultrahigh frame rate (1 KHz per slice) to scan the entire volume of a rat's kidney in vivo is demonstrated. The system, which can visualize the full 3D renal vascular branching pyramid at a resolution of 167 µm without any contrast agent, is used to chronically and noninvasively monitor kidneys with acute kidney injury (AKI, 3 days) and diabetic kidney disease (DKD, 8 weeks). Multiparametric UFD analyses (e.g., vessel volume occupancy (VVO), fractional moving blood volume (FMBV), vessel number density (VND), and vessel tortuosity (VT)) describe rapid vascular rarefaction from AKI and long‐term vascular degeneration from DKD, while the renal pathogeneses are validated by in vitro blood serum testing and stained histopathology. This work demonstrates the potential of 3D renal UFD to offer valuable insights into assessing kidney perfusion levels for future research in diabetes and kidney transplantation.

show abstract

Hepatocyte Injury Induced by Contrast‐Enhanced Diagnostic Ultrasound

Miller

Fabiilli

et al. 2018

J of Ultrasound Medicine

Self Cite

View full text Add to dashboard Cite

Objectives-Contrast-enhanced diagnostic ultrasound (US) has a potential to induce localized biological effects. The potential for contrast-enhanced diagnostic US bioeffects in liver were researched, with guidance from a report by Yang et al (Ultrasonics 2012; 52:1065-1071.Methods-Contact and standoff scanning was performed for 10 minutes with a diagnostic US phased array at 1.6 MHz during bolus injection or infusion of a contrast agent at a high dose. The impact of the imaging on rat liver was investigated by measuring enzyme release, microvascular leakage, and staining of injured hepatocytes.Results-The results showed liver enzyme release at 30 minutes, indicating liver injury, and elevated extraction of Evans blue dye, indicating microvascular leakage. In addition, Evans blue and trypan blue vital-staining methods revealed scattered stained cells within the US scan plane. For the Evans blue method, fluorescent cell counts in frozen sections were greatest for standoff exposure with contrast infusion. The count decreased strongly with depth for bolus injection, which was probably reflective of the high attenuation noted for this agent delivery method. Conclusions-The results qualitatively confirmed the report by Yang et al and additionally showed hepatocyte vital staining. Research is needed to determine the threshold for the effects and the contrast agent dose response.

show abstract

An in vivo rat model simulating imaging of human kidney by diagnostic ultrasound with gas-body contrast agent

Cited by 31 publications

References 18 publications

Frequency Dependence of Kidney Injury Induced by Contrast-Aided Diagnostic Ultrasound in Rats

Frequency Dependence of Kidney Injury Induced by Contrast-Aided Diagnostic Ultrasound in Rats

Contrast Agent‐Free 3D Renal Ultrafast Doppler Imaging Reveals Vascular Dysfunction in Acute and Diabetic Kidney Diseases

Hepatocyte Injury Induced by Contrast‐Enhanced Diagnostic Ultrasound

Contact Info

Product

Resources

About