An indirect competitive enzyme-linked immunosorbent assay for the determination of 3,4-dichlorobiphenyl in sediment using a specific polyclonal antibody

Yang, Gangyi; Zhuang, Huisheng; Chen, Hanyu; Ping, Xianyin

doi:10.1039/c3ay41993b

Cited by 7 publications

(9 citation statements)

References 39 publications

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“…Enzyme-linked immunosorbent assay (ELISA) is a simple and rapid method based on antigen and antibody recognition and is highly specific for particular residues [67,68]. There are various forms of ELISA methods.…”

Section: Enzyme-linked Immunosorbent Assay (Elisa)mentioning

confidence: 99%

Antibody production and application for immunoassay development of environmental hormones: a review

Tian

Wang

Lei

et al. 2018

Chem. Biol. Technol. Agric.

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Environmental hormones, also called environmental endocrine-disrupting chemicals (EDCs), are produced by various human activities and environment pollution, and they are composed of industrial chemicals and environmental pollutants. They play a harmful role in breaking the balance of normal physiological metabolism, causing serious diseases and disrupting reproductive development in wildlife and human beings by inhibiting or imitating the action of normal gonadal hormones. Strict controls of maximum residue levels to some environmental hormones in foodstuff have been set up by many countries. Traditional detection methods, due to their high accuracy and maturity, are used extensively, such as HPLC, HPLC-MS, and GC-MS. However, these methods are time consuming and require extraordinary skills of operators, and sometimes they cannot meet the requirements of field testing. Immunoassay has the advantages of high specificity, sensitivity, simplicity, convenience, and the ability to achieve high throughput, playing an irreplaceable role in the field of rapid detection. This review focuses on the antibody production and the development of immunoassays to detect environmental hormones in food and environment. Therefore, estrogen, progesterone, and testosterone are selected as examples to introduce a series of procedures for the detection of environmental hormones including antigen synthesis, antibody production, and antibody-based detection methods.

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Section: Enzyme-linked Immunosorbent Assay (Elisa)mentioning

confidence: 99%

Antibody production and application for immunoassay development of environmental hormones: a review

Tian

Wang

Lei

et al. 2018

Chem. Biol. Technol. Agric.

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“…Binding affinity of fluorescent LNA/DNA probes to complementary DNA/RNA targets was evaluated in a medium salt phosphate buffer (110 mM [Na + ], pH 7.0) and showed increased thermal denaturation (T m ) values for the modified duplexes compared to the reference (Table 1). This is an important result for application of the formed duplexes in diagnostics with shorter incubation steps at elevated temperatures and in various buffer systems [50,51]. Next, to assess the potential of the novel probes in diagnostics of clinically important autoantibodies, fluorescence homogeneous detection of human anti-dsDNAs was performed ( Figure 3) [10].…”

Section: Fluorescent Oligonucleotides In Direct Sensing Of Autoantibomentioning

confidence: 99%

Synthetic Oligonucleotide Probes for Detection of Autoimmune Antibodies

Astakhova¹

2014

J Clin Cell Immunol

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In this review, applications of synthetic oligonucleotide probes in diagnostics and studies of autoimmune antibodies against double-stranded DNA (anti-dsDNA) are described. As outlined herein, synthetic oligonucleotides and monoclonal antibodies provide appealing opportunities to develop standard simple assays for detection of anti-dsDNAs. Recent examples of anti-dsDNA detection using synthetic nucleic acid antigens include those applying surface plasmon resonance (SPR), antibody microarrays and homogeneous, or all-in-solution, detection with fluorescently-labelled probes. Since sequences of the applied nucleic acid antigens and monoclonal antibodies are known, the major benefit of these assays is the ability for the first time to clearly define the structural factors that govern the formation and stability of anti-dsDNA complexes. This is an essential first step toward understanding immune-complex mediated tissue injury in autoimmune conditions such as systemic lupus erythematosus (SLE) and arthritis, which involve production of pathogenic anti-dsDNAs. Moreover, synthetic nucleic acids and monoclonal antibodies have been demonstrated as key tools for the development of novel nucleic acid sensors for serotyping, along with studies of specificity and avidity of the antibody-dsDNA binding process. Among other antigens, fluorescent oligonucleotides prepared by click chemistry between novel alkyne-modified locked nucleic acid (LNA) strands and a series of fluorescent azides are proved to be very promising tools for efficient homogeneous detection of anti-dsDNAs.

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“…The design of a hapten plays a crucial role in generating high-affinity antibodies [28,29], and multiple factors need to be considered, such as their immunogenicity, affinity, and ability to bind to the target molecule. Generally, an appropriate hapten should mimic the target molecule in terms of structural resemblance, size, steric conformation, electronic configuration, and hydrophobic property [30,31]. Through the rational design of a hapten, the binding of antibodies to the target molecule can be made more stable, thereby improving the affinity and specificity of the antibodies.…”

Section: Introductionmentioning

confidence: 99%

An Enzyme Immunoassay Developed for the Determination of Methylene Blue in Aquatic Products Based on a Novel Hapten

Huang,

Yang,

Tang

et al. 2024

Chemosensors

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Methylene blue (MB) is a chemical dye which is used as an alternative drug for malachite green. In this study, based on computational chemistry, a novel hapten (TM) of MB was designed by comparing the conformational and electronic properties of MB. TM was synthesized and further conjugated with bovine serum albumin (BSA) and Ovalbumin (OVA). Then, the polyclonal antibodies were obtained by immunization with the immunogen of TM-BSA. Under optimal conditions, a sensitive indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed for the determination of MB, with an IC50 value of 41.5 μg L−1 and displaying a cross reaction of 78.2~88.9% with three different MB metabolins. The limit of detection (LOD) and the limit of quantitation (LOQ) for MB were validated to be 4.8 µg/kg and 6.0 µg/kg, respectively. Spiking experiments showed recoveries between 82.3% and 84.3%, with a relative standard deviation (RSD) of ≤6%. The results showed the generated polyclonal antibodies from the new hapten TM were reliable and could be used for detecting MB in fishery productions.

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An indirect competitive enzyme-linked immunosorbent assay for the determination of 3,4-dichlorobiphenyl in sediment using a specific polyclonal antibody

Abstract: A specific polyclonal antibody targeting non-dioxin-like PCB 3,4-dichlorobiphenyl (PCB12) was obtained, and a sensitive ic-ELISA was developed for the determination of PCB12 in sediment samples.

Cited by 7 publications

References 39 publications

Antibody production and application for immunoassay development of environmental hormones: a review

Antibody production and application for immunoassay development of environmental hormones: a review

Synthetic Oligonucleotide Probes for Detection of Autoimmune Antibodies

An Enzyme Immunoassay Developed for the Determination of Methylene Blue in Aquatic Products Based on a Novel Hapten

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